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Q45UF1

- NSP4_ROTJ1

UniProt

Q45UF1 - NSP4_ROTJ1

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Protein

Non-structural glycoprotein 4

Gene
N/A
Organism
Rotavirus B (isolate Human/China/ADRV-N J19/1997) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-J19))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in virus morphogenesis. Functions as a receptor for the immature double-layered inner capsid particle (ICP) which transiently buds into the lumen of the rough endoplasmic reticulum during viral maturation (By similarity).By similarity
Enterotoxin that causes a phospholipase C-dependent elevation of the intracellular calcium concentration in host intestinal mucosa cells. Increased concentration of intracellular calcium disrupts the cytoskeleton and the tight junctions, raising the paracellular permeability. Potentiates chloride ion secretion through a calcium ion-dependent signaling pathway, inducing age-dependent diarrhea. To perform this enterotoxigenic role in vivo, NSP4 is probably released from infected enterocytes in a soluble form capable of diffusing within the intestinal lumen and interacting with the plasma membrane receptors on neighboring epithelial cells (By similarity).By similarity

GO - Biological processi

  1. pathogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Enterotoxin, Toxin

Keywords - Biological processi

Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural glycoprotein 4
Short name:
NSP4
OrganismiRotavirus B (isolate Human/China/ADRV-N J19/1997) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-J19))
Taxonomic identifieri335103 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusunclassified rotaviruses
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000007663: Genome

Subcellular locationi

Chain Non-structural glycoprotein 4 : Host rough endoplasmic reticulum membrane By similarity; Single-pass type III membrane protein By similarity. Host membranehost caveola; Single-pass type III membrane protein. Secreted By similarity
Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles. NSP4 also localizes in vesicular structures, which contain an autophagosomal marker and associate with viroplasms in virus-infected cells (By similarity).By similarity

GO - Cellular componenti

  1. host cell endoplasmic reticulum Source: UniProtKB-KW
  2. host cell membrane Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host endoplasmic reticulum, Host membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 213213Non-structural glycoprotein 4PRO_0000369866Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi50 – 501N-linked (GlcNAc...); by hostSequence Analysis

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5555LumenalBy similarityAdd
BLAST
Topological domaini73 – 213141CytoplasmicBy similarityAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei56 – 7217HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili149 – 17527Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi124 – 1274Poly-Leu

Domaini

The coiled coil region mediates oligomerization.By similarity

Sequence similaritiesi

Belongs to the rotavirus NSP4 family.Curated

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Sequencei

Sequence statusi: Complete.

Q45UF1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEGTSESPVL DEFEVNNNDY DNDFISRFSQ NPLNAFSLFT DGNLQEYFMN
60 70 80 90 100
NSLEKIVIHV VLIVISLCGI KAQTSKIIYV VRLLFWKIYN VINNLVNKVI
110 120 130 140 150
NREKIINHQV VDNRFREFEE RFRLLLLQHD KNIAKQDDIV QYNKLDNFAE
160 170 180 190 200
SIKSEFNLKV AEMERRFQEL KWRCDMIANK AMNTIVLANT VDSNNKDEKI
210
VFDEGSVVQY NRE
Length:213
Mass (Da):25,111
Last modified:September 13, 2005 - v1
Checksum:i1A54C2E789ADD3FE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ113906 Genomic RNA. Translation: AAZ03494.1.
RefSeqiYP_392499.1. NC_007557.1.

Genome annotation databases

GeneIDi5076659.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ113906 Genomic RNA. Translation: AAZ03494.1 .
RefSeqi YP_392499.1. NC_007557.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 5076659.

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of a novel adult diarrhoea rotavirus strain J19 isolated in China and its significance for the evolution and origin of group B rotaviruses."
    Jiang S., Ji S., Tang Q., Cui X., Yang H., Kan B., Gao S.
    J. Gen. Virol. 89:2622-2629(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiNSP4_ROTJ1
AccessioniPrimary (citable) accession number: Q45UF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 13, 2005
Last modified: October 29, 2014
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3