Reviewed, UniProtKB/Swiss-Prot Q45QI7 (CFI_CAMSI)

Names and origin

Protein names

Recommended name:
    Chalcone--flavonone isomerase
      Short name=Chalcone isomerase
    EC=5.5.1.6

Gene names

Name:

CHI

Organism

Camellia sinensis (Tea)

Taxonomic identifier

4442 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › Ericales › Theaceae › Camellia

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the intramolecular cyclization of bicyclic chalcones into tricyclic (S)-flavanones. Responsible for the isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone) into naringenin By similarity.
Catalytic activity	A chalcone = a flavanone.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Miscellaneous	Part of the biosynthetic pathway for all classes of flavonoids, a large class of secondary plant metabolites, many of which are brightly colored.
Sequence similarities	Belongs to the chalcone isomerase family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Molecular function	Isomerase
Gene Ontology (GO)
Biological process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chalcone isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 230	230	Chalcone--flavonone isomerase		PRO_0000300832
Sites
	Binding site	52	1	Substrate By similarity
	Binding site	117	1	Substrate By similarity
	Binding site	194	1	Substrate By similarity
	Site	110	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q45QI7-1 [UniParc]. Last modified October 25, 2005. Version 2. Checksum: AACCE599B8E0F2A1	FASTA	230	24,723
	10 20 30 40 50 60 MSPSQSPSVA QVQIESHVFP PTVKPPGTSK PFFLGGAGER GLEIQGKFIK FTAIGVYLED 70 80 90 100 110 120 SAIPSLAVKW KGKTAEELTD SVDFFRDIVS GPFEKFTQVT MILPLTGQQY SEKVTENCVA 130 140 150 160 170 180 YWKAVGTYTD AEAKAIEKFI EVFKDETFPP GGSILFTQSP LGSLTIAFSK DGSLPETGTV 190 200 210 220 230 VMENKQLSEA VLESIIGKHG VSPAAKKSLA ARMSELLKEK PEAQTAAAAE

References

[1]	"Camellia sinensis cultivar UPASI-10 chalcone isomerase (CHI) mRNA." Rani A., Kumar S., Ahuja P.S. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. UPASI-10.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ120521 mRNA. Translation: AAZ17563.2.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	5.5.1.6. 568.
Family and domain databases
InterPro	IPR016088. Chalcone_isomerase_3-sand. IPR003466. Chalcone_isomerase_subgr. [Graphical view]
Gene3D	G3DSA:3.50.70.10. Chalcone. 1 hit.
Pfam	PF02431. Chalcone. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CFI_CAMSI

Accession

Primary (citable) accession number: Q45QI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	October 25, 2005
Last modified:	June 16, 2009
This is version 18 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)