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Q45FY6

- HPRT_PIG

UniProt

Q45FY6 - HPRT_PIG

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Protein

Hypoxanthine-guanine phosphoribosyltransferase

Gene

HPRT1

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway (By similarity).By similarity

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691GMPBy similarity
Active sitei138 – 1381Proton acceptorBy similarity
Binding sitei166 – 1661GMPBy similarity
Metal bindingi194 – 1941MagnesiumBy similarity
Binding sitei194 – 1941GMP; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi134 – 1429GMPBy similarity
Nucleotide bindingi186 – 1883GMPBy similarity

GO - Molecular functioni

  1. guanine phosphoribosyltransferase activity Source: UniProtKB
  2. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB
  3. magnesium ion binding Source: UniProtKB
  4. nucleotide binding Source: UniProtKB-KW
  5. protein homodimerization activity Source: UniProtKB

GO - Biological processi

  1. GMP catabolic process Source: UniProtKB
  2. guanine salvage Source: UniProtKB
  3. hypoxanthine metabolic process Source: UniProtKB
  4. hypoxanthine salvage Source: UniProtKB
  5. IMP metabolic process Source: UniProtKB
  6. IMP salvage Source: UniProtKB-UniPathway
  7. positive regulation of dopamine metabolic process Source: UniProtKB
  8. protein homotetramerization Source: UniProtKB
  9. purine nucleotide biosynthetic process Source: UniProtKB
  10. purine ribonucleoside salvage Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRT
Short name:
HGPRTase
Gene namesi
Name:HPRT1
Synonyms:HPRT
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Unplaced

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 218217Hypoxanthine-guanine phosphoribosyltransferasePRO_0000139590Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei103 – 1031N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ45FY6.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

IntActiQ45FY6. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ45FY6.
SMRiQ45FY6. Positions 5-218.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0634.
HOGENOMiHOG000236521.
HOVERGENiHBG000242.
InParanoidiQ45FY6.
KOiK00760.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q45FY6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATRSPSVVI SDDEPGYDLD LFCIPNHYAE DLEKVFIPHG LIMDRTERLA
60 70 80 90 100
RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI
110 120 130 140 150
RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV
160 170 180 190 200
KQHNPKMVKV ASLLVKRTPR SVGYRPDFVG FEIPDKFVVG YALDYNEYFR
210
DLNHVCVISE TGKAKYKA
Length:218
Mass (Da):24,555
Last modified:January 23, 2007 - v3
Checksum:i920FCBB948B2CFA4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ136030 mRNA. Translation: AAZ43258.1.
DQ845175 mRNA. Translation: ABI29189.1.
RefSeqiNP_001027548.1. NM_001032376.2.
UniGeneiSsc.4158.

Genome annotation databases

GeneIDi397351.
KEGGissc:397351.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ136030 mRNA. Translation: AAZ43258.1 .
DQ845175 mRNA. Translation: ABI29189.1 .
RefSeqi NP_001027548.1. NM_001032376.2.
UniGenei Ssc.4158.

3D structure databases

ProteinModelPortali Q45FY6.
SMRi Q45FY6. Positions 5-218.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q45FY6. 2 interactions.

Proteomic databases

PaxDbi Q45FY6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 397351.
KEGGi ssc:397351.

Organism-specific databases

CTDi 3251.

Phylogenomic databases

eggNOGi COG0634.
HOGENOMi HOG000236521.
HOVERGENi HBG000242.
InParanoidi Q45FY6.
KOi K00760.

Enzyme and pathway databases

UniPathwayi UPA00591 ; UER00648 .

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
InterProi IPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF00156. Pribosyltran. 1 hit.
[Graphical view ]
SUPFAMi SSF53271. SSF53271. 1 hit.
TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Gene targeting and chromosome engineering in porcine cells."
    Carlson D.F., Clark K.J., Shiroma D.S., Fahrenkrug S.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Selection of reference genes for gene expression studies in pig tissues using SYBR green qPCR."
    Nygaard A.-B., Joergensen C.B., Cirera S., Fredholm M.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiHPRT_PIG
AccessioniPrimary (citable) accession number: Q45FY6
Secondary accession number(s): A0SNU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 58 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3