Reviewed,
UniProtKB/Swiss-Prot Q45FX5 (VAV_CAEEL)
Last modified
June 16, 2009.
Version 34.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Protein vav-1 | ||||
| Gene names |
| ||||
| Organism | Caenorhabditis elegans [Complete proteome] | ||||
| Taxonomic identifier | 6239 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Protein attributes
| Sequence length | 1007 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Acts as guanine nucleotide exchange factor (GEF) for Rho GTPase. Has a critical roles in the generation of rhythmic behaviors: feeding, defecation and ovulation by dynamically regulating the concentration of intracellular calcium. Ref.1 |
| Tissue specificity | Strong expression in the pharynx, proximal gonad, spermatheca, intestine and rectal epithelia. Ref.1 |
| Domain | An acidic domain (AC) contains three highly conserved tyrosines that are involved in the autoinhibition of GEF activity By similarity. |
| Post-translational modification | GEF activity is regulated by phosphorylation on tyrosine residues. |
| Sequence similarities | Contains 1 CH (calponin-homology) domain. Contains 1 DH (DBL-homology) domain. Contains 1 PH domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 SH2 domain. Contains 2 SH3 domains. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| Q22847 | 1 | EBI-316741,EBI-316760 | ||
| pal-1 | P34766 | 1 | EBI-316741,EBI-311911 | |
| php-3 | Q9XW88 | 1 | EBI-316741,EBI-316766 |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform a (identifier: Q45FX5-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform b (identifier: Q45FX5-2) The sequence of this isoform differs from the canonical sequence as follows: 1-32: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1007 | 1007 | Protein vav-1 | PRO_0000238786 | |||||
Regions | |||||||||
| Domain | 37 – 150 | 114 | CH | ||||||
| Domain | 240 – 437 | 198 | DH | ||||||
| Domain | 470 – 598 | 129 | PH | ||||||
| Domain | 770 – 813 | 44 | SH3 1 | ||||||
| Domain | 831 – 925 | 95 | SH2 | ||||||
| Domain | 926 – 991 | 66 | SH3 2 | ||||||
| Zinc finger | 610 – 664 | 55 | Phorbol-ester/DAG-type | ||||||
| Region | 151 – 239 | 89 | AC | ||||||
| Compositional bias | 803 – 806 | 4 | Poly-Ser | ||||||
| Compositional bias | 1004 – 1007 | 4 | Poly-Ser | ||||||
Amino acid modifications | |||||||||
| Modified residue | 183 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 200 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 217 | 1 | Phosphotyrosine By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 32 | 32 | Missing in isoform b. | VSP_018992 | |||||
Experimental info | |||||||||
| Mutagenesis | 183 | 1 | Y → F: Leads to a dominant hypercontracted and uncoordinated phenotype; when associated with Y-200 and Y-217. Ref.1 | ||||||
| Mutagenesis | 200 | 1 | Y → F: Leads to a dominant hypercontracted and uncoordinated phenotype; when associated with Y-183 and Y-217. Ref.1 | ||||||
| Mutagenesis | 217 | 1 | Y → F: Leads to a dominant hypercontracted and uncoordinated phenotype. Leads to a dominant hypercontracted and uncoordinated phenotype; when associated with Y-183 and Y-200. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The Rho/Rac family guanine nucleotide exchange factor VAV-1 regulates rhythmic behaviors in Caenorhabditis elegans." Norman K.R., Fazzio R.T., Mellem J.E., Espelt M.V., Strange K., Beckerle M.C., Maricq A.V. Cell 123:119-132(2005) [PubMed: 16213217] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF TYR-183; TYR-200 AND TYR-217. |
| [2] | "Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| DQ136172 mRNA. Translation: AAZ66767.1. U23520 Genomic DNA. Translation: AAC46554.4. U23520 Genomic DNA. Translation: ABB88236.1. | |
| PIR | T15778. |
| RefSeq | NP_001041223.1. |
| UniGene | Cel.7082 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q45FX5. 6 interactions. |
Genome annotation databases | |
| Ensembl | C35B8.2. Caenorhabditis elegans. [Contig view] |
| GeneID | 181153. |
Organism-specific databases | |
| WormBase | WBGene00006887. vav-1. |
| WormPep | C35B8.2. CE38040. [WorfDB] |
Phylogenomic databases | |
| OMA | Q45FX5. PSMNEND. |
Gene expression databases | |
| ArrayExpress | Q45FX5. |
Family and domain databases | |
| InterPro | IPR001715. Calponin_act_bd. IPR002219. DAG_PE_bd. IPR000219. DH-domain. IPR001331. GDS_CDC24_CS. IPR011993. PH_type. IPR001720. PI3kinase_P85. IPR001849. Pleckstrin_homology. IPR000980. SH2. IPR011511. SH3_2. IPR001452. SH3_domain. [Graphical view] |
| Gene3D | G3DSA:1.10.418.10. Calponin-homology. 1 hit. G3DSA:2.30.29.30. PH_type. 1 hit. G3DSA:1.20.900.10. RhoGEF. 1 hit. G3DSA:3.30.505.10. SH2. 1 hit. |
| Pfam | PF00130. C1_1. 1 hit. PF00307. CH. 1 hit. PF00169. PH. 1 hit. PF00621. RhoGEF. 1 hit. PF00017. SH2. 1 hit. PF00018. SH3_1. 1 hit. PF07653. SH3_2. 1 hit. [Graphical view] |
| PRINTS | PR00678. PI3KINASEP85. PR00401. SH2DOMAIN. |
| ProDom | PD000093. SH2. 1 hit. PD000066. SH3. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00109. C1. 1 hit. SM00033. CH. 1 hit. SM00233. PH. 1 hit. SM00325. RhoGEF. 1 hit. SM00252. SH2. 1 hit. SM00326. SH3. 1 hit. [Graphical view] |
| PROSITE | PS50021. CH. 1 hit. PS00741. DH_1. 1 hit. PS50010. DH_2. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS50001. SH2. 1 hit. PS50002. SH3. 1 hit. PS00479. ZF_DAG_PE_1. 1 hit. PS50081. ZF_DAG_PE_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 912636. |
Entry information
| Entry name | VAV_CAEEL | ||||||||
| Accession | Primary (citable) accession number: Q45FX5 Secondary accession number(s): Q18479 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | Caenorhabditis annotation project | ||||||||
Relevant documents
| Caenorhabditis elegans Caenorhabditis elegans: entries, gene names and cross-references to WormPep |
| Protein Spotlight Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries |
| SIMILARITY comments Index of protein domains and families |
