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Q45FX5 (VAV_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein vav-1
Gene names
Name:vav-1
ORF Names:C35B8.2
OrganismCaenorhabditis elegans
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length1007 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as guanine nucleotide exchange factor (GEF) for Rho GTPase. Has a critical roles in the generation of rhythmic behaviors: feeding, defecation and ovulation by dynamically regulating the concentration of intracellular calcium. Ref.1

Tissue specificity

Strong expression in the pharynx, proximal gonad, spermatheca, intestine and rectal epithelia. Ref.1

Domain

An acidic domain (AC) contains three highly conserved tyrosines that are involved in the autoinhibition of GEF activity By similarity.

Post-translational modification

GEF activity is regulated by phosphorylation on tyrosine residues.

Sequence similarities

Contains 1 CH (calponin-homology) domain.

Contains 1 DH (DBL-homology) domain.

Contains 1 PH domain.

Contains 1 phorbol-ester/DAG-type zinc finger.

Contains 1 SH2 domain.

Contains 2 SH3 domains.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: Q45FX5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform b (identifier: Q45FX5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10071007Protein vav-1
PRO_0000238786

Regions

Domain37 – 150114CH
Domain240 – 437198DH
Domain470 – 598129PH
Domain770 – 81344SH3 1
Domain831 – 92595SH2
Domain926 – 99166SH3 2
Zinc finger610 – 66455Phorbol-ester/DAG-type
Region151 – 23989AC
Compositional bias803 – 8064Poly-Ser
Compositional bias1004 – 10074Poly-Ser

Amino acid modifications

Modified residue1831Phosphotyrosine By similarity
Modified residue2001Phosphotyrosine By similarity
Modified residue2171Phosphotyrosine By similarity

Natural variations

Alternative sequence1 – 3232Missing in isoform b.
VSP_018992

Experimental info

Mutagenesis1831Y → F: Leads to a dominant hypercontracted and uncoordinated phenotype; when associated with Y-200 and Y-217. Ref.1
Mutagenesis2001Y → F: Leads to a dominant hypercontracted and uncoordinated phenotype; when associated with Y-183 and Y-217. Ref.1
Mutagenesis2171Y → F: Leads to a dominant hypercontracted and uncoordinated phenotype. Leads to a dominant hypercontracted and uncoordinated phenotype; when associated with Y-183 and Y-200. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 4B0E7A79C7BB2DFD

FASTA1,007113,554
        10         20         30         40         50         60 
MSRRSTTTST NFGLSWSLVD VISSSTAVFK VPMNGGCDLW IGCARWLRDM KVLTTDKNGT 

        70         80         90        100        110        120 
MLEFASVLRD GILLCRLANT LVPNGIDQKK IMRTNQPSPF LCCNNINYFA MFCKTYFNLE 

       130        140        150        160        170        180 
DADLFTAEDL YYMNGFQKVL KTLSFLSHTK ESLSRGVDPF PDTDNNQEGT SNGSEFEDDV 

       190        200        210        220        230        240 
EIYQSLHDNI ENVDPNRTIY GPITSADPEE QQSEQLYDRI VTNRKPSMNE NDLQNTPTLK 

       250        260        270        280        290        300 
RNRCIRELYD TEKNYVAQAL VTIIKTFYEP LKGIIPTSDY NIIFGNIEEI NVLHTALLAD 

       310        320        330        340        350        360 
LEYPVKVALG LSDATPPRPI SLNECVPQTI GEVFIKYRDQ FLAYGKYCSN LPDSRKLSNE 

       370        380        390        400        410        420 
LLKTNEFISR NINELTAQGN CKFGMNDLLC VPFQRLTKYP LLLKELQKKT DLASPDRKSL 

       430        440        450        460        470        480 
EEAVEVMEDV CNYINEESRD TNAIKVIDEI EQSITDLSMP LNVKLHDYGR VNLDGEVKMA 

       490        500        510        520        530        540 
ESTLTQAGKP KQRYIFLFDK VIVVCKAANK VMAAKTTGAS ARTNTFTYKN AYVMSELTID 

       550        560        570        580        590        600 
KNASLDVKSG GTITRRTQYV IIMTRDRNEN NEITQLTFYF KNEATRNNWM TALLLSKSNV 

       610        620        630        640        650        660 
SPTDYLRDTN HKVAFHSFRV DVKNPATCDV CDKLMKGLQY QGYKCESCNM SMHKECLGLK 

       670        680        690        700        710        720 
KCEAVRKSTH ETRSSQSFNC NRPRFHIHEG DIVVANSNST PSDLSYLQFA KGDRIEVIKM 

       730        740        750        760        770        780 
QGHNRFTGCL INNRNRTGLV HLDHVSQSRT TSMIGLSPID SPAGSIAPRV VRNESTVLPN 

       790        800        810        820        830        840 
KLLSDGSSRS LSGPHGSRSS RNSSSSTING SMDSVPRQQD YVNTEISEFL WYMGEMERAK 

       850        860        870        880        890        900 
AESTLKGTPN GTFLVRYSKN RKQTAISLSY KNDVKHMIIE QNSDGKVYLD EDYIFNSTVE 

       910        920        930        940        950        960 
LVQYYRSNNL IEIFAALDTC LKNPYSQCKV FKAVHDYDAP SPNNEGKFLS FKTGDIVVLL 

       970        980        990       1000 
DTVGEDRGWW KGQVNNKSGF FPLSYVKPYD PATEGSSSPV TPTSSSS

« Hide

Isoform b [UniParc].

Checksum: A0A0C4862F56F3B0
Show »

FASTA975110,109

References

« Hide 'large scale' references
[1]"The Rho/Rac family guanine nucleotide exchange factor VAV-1 regulates rhythmic behaviors in Caenorhabditis elegans."
Norman K.R., Fazzio R.T., Mellem J.E., Espelt M.V., Strange K., Beckerle M.C., Maricq A.V.
Cell 123:119-132(2005) [PubMed: 16213217] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF TYR-183; TYR-200 AND TYR-217.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
+Additional computationally mapped references.

Web resources

Protein Spotlight

Nematode tempo - Issue 71 of June 2006

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		DQ136172 mRNA. Translation: AAZ66767.1.
FO080787 Genomic DNA. Translation: CCD66769.1.
FO080787 Genomic DNA. Translation: CCD66770.1.
PIRT15778.
RefSeqNP_001041222.1. NM_001047757.1.
NP_001041223.1. NM_001047758.1.
UniGeneCel.7082.

3D structure databases

ProteinModelPortalQ45FX5.
SMRQ45FX5. Positions 33-665, 796-991.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-25959N.
IntActQ45FX5. 3 interactions.
MINTMINT-1070973.
STRINGQ45FX5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaC35B8.2b; C35B8.2b; C35B8.2.
GeneID181153.
KEGGcel:C35B8.2.
UCSCC35B8.2a.1. c. elegans.

Organism-specific databases

CTD181153.
WormBaseC35B8.2a; CE38040; WBGene00006887; vav-1.
C35B8.2b; CE39333; WBGene00006887; vav-1.

Phylogenomic databases

eggNOGmeNOG04277.
GeneTreeEMGT00050000003921.
HOGENOMHBG389558.
InParanoidQ45FX5.
OMAVELVQYY.
PhylomeDBQ45FX5.

Gene expression databases

ArrayExpressQ45FX5.

Family and domain databases

InterProIPR001715. CH-domain.
IPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR011993. PH_type.
IPR001849. Pleckstrin_homology.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
IPR000980. SH2.
IPR011511. SH3_2.
IPR001452. SH3_domain.
IPR013315. Spectrin_alpha_SH3.
[Graphical view]
Gene3DG3DSA:1.10.418.10. Calponin-homology. 1 hit.
G3DSA:2.30.29.30. PH_type. 1 hit.
G3DSA:1.20.900.10. RhoGEF. 1 hit.
G3DSA:3.30.505.10. SH2. 1 hit.
KOK05730.
PfamPF00130. C1_1. 1 hit.
PF00307. CH. 1 hit.
PF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
PF00017. SH2. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
PRINTSPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR01887. SPECTRNALPHA.
SMARTSM00109. C1. 1 hit.
SM00033. CH. 1 hit.
SM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
SM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF47576. Calponin-homology. 1 hit.
SSF48065. DH-domain. 1 hit.
SSF50044. SH3. 2 hits.
PROSITEPS50021. CH. 1 hit.
PS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio912636.

Entry information

Entry nameVAV_CAEEL
AccessionPrimary (citable) accession number: Q45FX5
Secondary accession number(s): Q18479
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: September 13, 2005
Last modified: December 14, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents