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Q45FX5

- VAV_CAEEL

UniProt

Q45FX5 - VAV_CAEEL

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Protein

Protein vav-1

Gene

vav-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as guanine nucleotide exchange factor (GEF) for Rho GTPase. Has a critical roles in the generation of rhythmic behaviors: feeding, defecation and ovulation by dynamically regulating the concentration of intracellular calcium.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri610 – 66455Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. Rho guanyl-nucleotide exchange factor activity Source: InterPro

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
  2. negative regulation of Notch signaling pathway Source: WormBase
  3. regulation of vulval development Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_106875. Interleukin-3, 5 and GM-CSF signaling.
REACT_184089. FCERI mediated MAPK activation.
REACT_184105. Signaling by SCF-KIT.
REACT_184107. DAP12 signaling.
REACT_184335. FCERI mediated Ca+2 mobilization.
REACT_232673. VEGFR2 mediated vascular permeability.
REACT_234237. EPH-ephrin mediated repulsion of cells.
REACT_234361. Regulation of signaling by CBL.
REACT_236360. Signal transduction by L1.
REACT_246059. Rho GTPase cycle.
REACT_246696. VEGFA-VEGFR2 Pathway.
REACT_248761. GPVI-mediated activation cascade.
REACT_250475. G alpha (12/13) signalling events.
REACT_253015. NRAGE signals death through JNK.
SignaLinkiQ45FX5.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein vav-1
Gene namesi
Name:vav-1
ORF Names:C35B8.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome X

Organism-specific databases

WormBaseiC35B8.2a; CE38040; WBGene00006887; vav-1.
C35B8.2b; CE39333; WBGene00006887; vav-1.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi183 – 1831Y → F: Leads to a dominant hypercontracted and uncoordinated phenotype; when associated with Y-200 and Y-217. 1 Publication
Mutagenesisi200 – 2001Y → F: Leads to a dominant hypercontracted and uncoordinated phenotype; when associated with Y-183 and Y-217. 1 Publication
Mutagenesisi217 – 2171Y → F: Leads to a dominant hypercontracted and uncoordinated phenotype. Leads to a dominant hypercontracted and uncoordinated phenotype; when associated with Y-183 and Y-200. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10071007Protein vav-1PRO_0000238786Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei183 – 1831PhosphotyrosineBy similarity
Modified residuei200 – 2001PhosphotyrosineBy similarity
Modified residuei217 – 2171PhosphotyrosineBy similarity

Post-translational modificationi

GEF activity is regulated by phosphorylation on tyrosine residues.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ45FX5.

Expressioni

Tissue specificityi

Strong expression in the pharynx, proximal gonad, spermatheca, intestine and rectal epithelia.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi46070. 6 interactions.
DIPiDIP-25959N.
IntActiQ45FX5. 27 interactions.
MINTiMINT-1070973.

Structurei

3D structure databases

ProteinModelPortaliQ45FX5.
SMRiQ45FX5. Positions 34-658, 801-990.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 150114CHPROSITE-ProRule annotationAdd
BLAST
Domaini240 – 437198DHPROSITE-ProRule annotationAdd
BLAST
Domaini470 – 598129PHPROSITE-ProRule annotationAdd
BLAST
Domaini770 – 81344SH3 1PROSITE-ProRule annotationAdd
BLAST
Domaini831 – 92595SH2PROSITE-ProRule annotationAdd
BLAST
Domaini926 – 99166SH3 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni151 – 23989ACAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi803 – 8064Poly-Ser
Compositional biasi1004 – 10074Poly-Ser

Domaini

An acidic domain (AC) contains three highly conserved tyrosines that are involved in the autoinhibition of GEF activity.By similarity

Sequence similaritiesi

Contains 1 CH (calponin-homology) domain.PROSITE-ProRule annotation
Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation
Contains 2 SH3 domains.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri610 – 66455Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, SH2 domain, SH3 domain, Zinc-finger

Phylogenomic databases

eggNOGiCOG5422.
GeneTreeiENSGT00740000115307.
HOGENOMiHOG000020846.
InParanoidiQ45FX5.
KOiK05730.
OMAiVELVQYY.
OrthoDBiEOG78SQH7.
PhylomeDBiQ45FX5.

Family and domain databases

Gene3Di1.10.418.10. 1 hit.
1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR001715. CH-domain.
IPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR002219. PE/DAG-bd.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR000980. SH2.
IPR011511. SH3_2.
IPR001452. SH3_domain.
IPR013315. Spectrin_alpha_SH3.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF00307. CH. 1 hit.
PF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
PF00017. SH2. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR01887. SPECTRNALPHA.
SMARTiSM00109. C1. 1 hit.
SM00033. CH. 1 hit.
SM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
SM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
SSF48065. SSF48065. 1 hit.
SSF50044. SSF50044. 2 hits.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50021. CH. 1 hit.
PS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform a (identifier: Q45FX5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRRSTTTST NFGLSWSLVD VISSSTAVFK VPMNGGCDLW IGCARWLRDM
60 70 80 90 100
KVLTTDKNGT MLEFASVLRD GILLCRLANT LVPNGIDQKK IMRTNQPSPF
110 120 130 140 150
LCCNNINYFA MFCKTYFNLE DADLFTAEDL YYMNGFQKVL KTLSFLSHTK
160 170 180 190 200
ESLSRGVDPF PDTDNNQEGT SNGSEFEDDV EIYQSLHDNI ENVDPNRTIY
210 220 230 240 250
GPITSADPEE QQSEQLYDRI VTNRKPSMNE NDLQNTPTLK RNRCIRELYD
260 270 280 290 300
TEKNYVAQAL VTIIKTFYEP LKGIIPTSDY NIIFGNIEEI NVLHTALLAD
310 320 330 340 350
LEYPVKVALG LSDATPPRPI SLNECVPQTI GEVFIKYRDQ FLAYGKYCSN
360 370 380 390 400
LPDSRKLSNE LLKTNEFISR NINELTAQGN CKFGMNDLLC VPFQRLTKYP
410 420 430 440 450
LLLKELQKKT DLASPDRKSL EEAVEVMEDV CNYINEESRD TNAIKVIDEI
460 470 480 490 500
EQSITDLSMP LNVKLHDYGR VNLDGEVKMA ESTLTQAGKP KQRYIFLFDK
510 520 530 540 550
VIVVCKAANK VMAAKTTGAS ARTNTFTYKN AYVMSELTID KNASLDVKSG
560 570 580 590 600
GTITRRTQYV IIMTRDRNEN NEITQLTFYF KNEATRNNWM TALLLSKSNV
610 620 630 640 650
SPTDYLRDTN HKVAFHSFRV DVKNPATCDV CDKLMKGLQY QGYKCESCNM
660 670 680 690 700
SMHKECLGLK KCEAVRKSTH ETRSSQSFNC NRPRFHIHEG DIVVANSNST
710 720 730 740 750
PSDLSYLQFA KGDRIEVIKM QGHNRFTGCL INNRNRTGLV HLDHVSQSRT
760 770 780 790 800
TSMIGLSPID SPAGSIAPRV VRNESTVLPN KLLSDGSSRS LSGPHGSRSS
810 820 830 840 850
RNSSSSTING SMDSVPRQQD YVNTEISEFL WYMGEMERAK AESTLKGTPN
860 870 880 890 900
GTFLVRYSKN RKQTAISLSY KNDVKHMIIE QNSDGKVYLD EDYIFNSTVE
910 920 930 940 950
LVQYYRSNNL IEIFAALDTC LKNPYSQCKV FKAVHDYDAP SPNNEGKFLS
960 970 980 990 1000
FKTGDIVVLL DTVGEDRGWW KGQVNNKSGF FPLSYVKPYD PATEGSSSPV

TPTSSSS
Length:1,007
Mass (Da):113,554
Last modified:September 13, 2005 - v1
Checksum:i4B0E7A79C7BB2DFD
GO
Isoform b (identifier: Q45FX5-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: Missing.

Show »
Length:975
Mass (Da):110,109
Checksum:iA0A0C4862F56F3B0
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3232Missing in isoform b. CuratedVSP_018992Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ136172 mRNA. Translation: AAZ66767.1.
FO080787 Genomic DNA. Translation: CCD66769.1.
FO080787 Genomic DNA. Translation: CCD66770.1.
PIRiT15778.
RefSeqiNP_001041222.1. NM_001047757.2. [Q45FX5-2]
NP_001041223.1. NM_001047758.1. [Q45FX5-1]
UniGeneiCel.7082.

Genome annotation databases

EnsemblMetazoaiC35B8.2b; C35B8.2b; WBGene00006887. [Q45FX5-1]
GeneIDi181153.
KEGGicel:CELE_C35B8.2.
UCSCiC35B8.2a.1. c. elegans. [Q45FX5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Protein Spotlight

Nematode tempo - Issue 71 of June 2006

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ136172 mRNA. Translation: AAZ66767.1 .
FO080787 Genomic DNA. Translation: CCD66769.1 .
FO080787 Genomic DNA. Translation: CCD66770.1 .
PIRi T15778.
RefSeqi NP_001041222.1. NM_001047757.2. [Q45FX5-2 ]
NP_001041223.1. NM_001047758.1. [Q45FX5-1 ]
UniGenei Cel.7082.

3D structure databases

ProteinModelPortali Q45FX5.
SMRi Q45FX5. Positions 34-658, 801-990.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 46070. 6 interactions.
DIPi DIP-25959N.
IntActi Q45FX5. 27 interactions.
MINTi MINT-1070973.

Proteomic databases

PaxDbi Q45FX5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai C35B8.2b ; C35B8.2b ; WBGene00006887 . [Q45FX5-1 ]
GeneIDi 181153.
KEGGi cel:CELE_C35B8.2.
UCSCi C35B8.2a.1. c. elegans. [Q45FX5-1 ]

Organism-specific databases

CTDi 181153.
WormBasei C35B8.2a ; CE38040 ; WBGene00006887 ; vav-1.
C35B8.2b ; CE39333 ; WBGene00006887 ; vav-1.

Phylogenomic databases

eggNOGi COG5422.
GeneTreei ENSGT00740000115307.
HOGENOMi HOG000020846.
InParanoidi Q45FX5.
KOi K05730.
OMAi VELVQYY.
OrthoDBi EOG78SQH7.
PhylomeDBi Q45FX5.

Enzyme and pathway databases

Reactomei REACT_106875. Interleukin-3, 5 and GM-CSF signaling.
REACT_184089. FCERI mediated MAPK activation.
REACT_184105. Signaling by SCF-KIT.
REACT_184107. DAP12 signaling.
REACT_184335. FCERI mediated Ca+2 mobilization.
REACT_232673. VEGFR2 mediated vascular permeability.
REACT_234237. EPH-ephrin mediated repulsion of cells.
REACT_234361. Regulation of signaling by CBL.
REACT_236360. Signal transduction by L1.
REACT_246059. Rho GTPase cycle.
REACT_246696. VEGFA-VEGFR2 Pathway.
REACT_248761. GPVI-mediated activation cascade.
REACT_250475. G alpha (12/13) signalling events.
REACT_253015. NRAGE signals death through JNK.
SignaLinki Q45FX5.

Miscellaneous databases

NextBioi 912636.

Family and domain databases

Gene3Di 1.10.418.10. 1 hit.
1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
3.30.505.10. 1 hit.
InterProi IPR001715. CH-domain.
IPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR002219. PE/DAG-bd.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR000980. SH2.
IPR011511. SH3_2.
IPR001452. SH3_domain.
IPR013315. Spectrin_alpha_SH3.
[Graphical view ]
Pfami PF00130. C1_1. 1 hit.
PF00307. CH. 1 hit.
PF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
PF00017. SH2. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view ]
PRINTSi PR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR01887. SPECTRNALPHA.
SMARTi SM00109. C1. 1 hit.
SM00033. CH. 1 hit.
SM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
SM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view ]
SUPFAMi SSF47576. SSF47576. 1 hit.
SSF48065. SSF48065. 1 hit.
SSF50044. SSF50044. 2 hits.
SSF55550. SSF55550. 1 hit.
PROSITEi PS50021. CH. 1 hit.
PS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Rho/Rac family guanine nucleotide exchange factor VAV-1 regulates rhythmic behaviors in Caenorhabditis elegans."
    Norman K.R., Fazzio R.T., Mellem J.E., Espelt M.V., Strange K., Beckerle M.C., Maricq A.V.
    Cell 123:119-132(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF TYR-183; TYR-200 AND TYR-217.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.

Entry informationi

Entry nameiVAV_CAEEL
AccessioniPrimary (citable) accession number: Q45FX5
Secondary accession number(s): Q18479
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: September 13, 2005
Last modified: November 26, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3