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Q45FX5

- VAV_CAEEL

UniProt

Q45FX5 - VAV_CAEEL

Protein

Protein vav-1

Gene

vav-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Acts as guanine nucleotide exchange factor (GEF) for Rho GTPase. Has a critical roles in the generation of rhythmic behaviors: feeding, defecation and ovulation by dynamically regulating the concentration of intracellular calcium.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri610 – 66455Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. Rho guanyl-nucleotide exchange factor activity Source: InterPro

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. negative regulation of Notch signaling pathway Source: WormBase
    3. regulation of vulval development Source: WormBase

    Keywords - Molecular functioni

    Guanine-nucleotide releasing factor

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    SignaLinkiQ45FX5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein vav-1
    Gene namesi
    Name:vav-1
    ORF Names:C35B8.2
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome X

    Organism-specific databases

    WormBaseiC35B8.2a; CE38040; WBGene00006887; vav-1.
    C35B8.2b; CE39333; WBGene00006887; vav-1.

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi183 – 1831Y → F: Leads to a dominant hypercontracted and uncoordinated phenotype; when associated with Y-200 and Y-217. 1 Publication
    Mutagenesisi200 – 2001Y → F: Leads to a dominant hypercontracted and uncoordinated phenotype; when associated with Y-183 and Y-217. 1 Publication
    Mutagenesisi217 – 2171Y → F: Leads to a dominant hypercontracted and uncoordinated phenotype. Leads to a dominant hypercontracted and uncoordinated phenotype; when associated with Y-183 and Y-200. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10071007Protein vav-1PRO_0000238786Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei183 – 1831PhosphotyrosineBy similarity
    Modified residuei200 – 2001PhosphotyrosineBy similarity
    Modified residuei217 – 2171PhosphotyrosineBy similarity

    Post-translational modificationi

    GEF activity is regulated by phosphorylation on tyrosine residues.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ45FX5.

    Expressioni

    Tissue specificityi

    Strong expression in the pharynx, proximal gonad, spermatheca, intestine and rectal epithelia.1 Publication

    Interactioni

    Protein-protein interaction databases

    BioGridi46070. 6 interactions.
    DIPiDIP-25959N.
    IntActiQ45FX5. 27 interactions.
    MINTiMINT-1070973.

    Structurei

    3D structure databases

    ProteinModelPortaliQ45FX5.
    SMRiQ45FX5. Positions 34-658, 801-990.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini37 – 150114CHPROSITE-ProRule annotationAdd
    BLAST
    Domaini240 – 437198DHPROSITE-ProRule annotationAdd
    BLAST
    Domaini470 – 598129PHPROSITE-ProRule annotationAdd
    BLAST
    Domaini770 – 81344SH3 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini831 – 92595SH2PROSITE-ProRule annotationAdd
    BLAST
    Domaini926 – 99166SH3 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni151 – 23989ACAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi803 – 8064Poly-Ser
    Compositional biasi1004 – 10074Poly-Ser

    Domaini

    An acidic domain (AC) contains three highly conserved tyrosines that are involved in the autoinhibition of GEF activity.By similarity

    Sequence similaritiesi

    Contains 1 CH (calponin-homology) domain.PROSITE-ProRule annotation
    Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
    Contains 1 SH2 domain.PROSITE-ProRule annotation
    Contains 2 SH3 domains.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri610 – 66455Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, SH2 domain, SH3 domain, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5422.
    GeneTreeiENSGT00740000115307.
    HOGENOMiHOG000020846.
    InParanoidiQ45FX5.
    KOiK05730.
    OMAiVELVQYY.
    OrthoDBiEOG78SQH7.
    PhylomeDBiQ45FX5.

    Family and domain databases

    Gene3Di1.10.418.10. 1 hit.
    1.20.900.10. 1 hit.
    2.30.29.30. 1 hit.
    3.30.505.10. 1 hit.
    InterProiIPR001715. CH-domain.
    IPR000219. DH-domain.
    IPR001331. GDS_CDC24_CS.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    IPR000980. SH2.
    IPR011511. SH3_2.
    IPR001452. SH3_domain.
    IPR013315. Spectrin_alpha_SH3.
    [Graphical view]
    PfamiPF00130. C1_1. 1 hit.
    PF00307. CH. 1 hit.
    PF00169. PH. 1 hit.
    PF00621. RhoGEF. 1 hit.
    PF00017. SH2. 1 hit.
    PF07653. SH3_2. 1 hit.
    [Graphical view]
    PRINTSiPR00401. SH2DOMAIN.
    PR00452. SH3DOMAIN.
    PR01887. SPECTRNALPHA.
    SMARTiSM00109. C1. 1 hit.
    SM00033. CH. 1 hit.
    SM00233. PH. 1 hit.
    SM00325. RhoGEF. 1 hit.
    SM00252. SH2. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF47576. SSF47576. 1 hit.
    SSF48065. SSF48065. 1 hit.
    SSF50044. SSF50044. 2 hits.
    SSF55550. SSF55550. 1 hit.
    PROSITEiPS50021. CH. 1 hit.
    PS00741. DH_1. 1 hit.
    PS50010. DH_2. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS50001. SH2. 1 hit.
    PS50002. SH3. 1 hit.
    PS00479. ZF_DAG_PE_1. 1 hit.
    PS50081. ZF_DAG_PE_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform a (identifier: Q45FX5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSRRSTTTST NFGLSWSLVD VISSSTAVFK VPMNGGCDLW IGCARWLRDM     50
    KVLTTDKNGT MLEFASVLRD GILLCRLANT LVPNGIDQKK IMRTNQPSPF 100
    LCCNNINYFA MFCKTYFNLE DADLFTAEDL YYMNGFQKVL KTLSFLSHTK 150
    ESLSRGVDPF PDTDNNQEGT SNGSEFEDDV EIYQSLHDNI ENVDPNRTIY 200
    GPITSADPEE QQSEQLYDRI VTNRKPSMNE NDLQNTPTLK RNRCIRELYD 250
    TEKNYVAQAL VTIIKTFYEP LKGIIPTSDY NIIFGNIEEI NVLHTALLAD 300
    LEYPVKVALG LSDATPPRPI SLNECVPQTI GEVFIKYRDQ FLAYGKYCSN 350
    LPDSRKLSNE LLKTNEFISR NINELTAQGN CKFGMNDLLC VPFQRLTKYP 400
    LLLKELQKKT DLASPDRKSL EEAVEVMEDV CNYINEESRD TNAIKVIDEI 450
    EQSITDLSMP LNVKLHDYGR VNLDGEVKMA ESTLTQAGKP KQRYIFLFDK 500
    VIVVCKAANK VMAAKTTGAS ARTNTFTYKN AYVMSELTID KNASLDVKSG 550
    GTITRRTQYV IIMTRDRNEN NEITQLTFYF KNEATRNNWM TALLLSKSNV 600
    SPTDYLRDTN HKVAFHSFRV DVKNPATCDV CDKLMKGLQY QGYKCESCNM 650
    SMHKECLGLK KCEAVRKSTH ETRSSQSFNC NRPRFHIHEG DIVVANSNST 700
    PSDLSYLQFA KGDRIEVIKM QGHNRFTGCL INNRNRTGLV HLDHVSQSRT 750
    TSMIGLSPID SPAGSIAPRV VRNESTVLPN KLLSDGSSRS LSGPHGSRSS 800
    RNSSSSTING SMDSVPRQQD YVNTEISEFL WYMGEMERAK AESTLKGTPN 850
    GTFLVRYSKN RKQTAISLSY KNDVKHMIIE QNSDGKVYLD EDYIFNSTVE 900
    LVQYYRSNNL IEIFAALDTC LKNPYSQCKV FKAVHDYDAP SPNNEGKFLS 950
    FKTGDIVVLL DTVGEDRGWW KGQVNNKSGF FPLSYVKPYD PATEGSSSPV 1000
    TPTSSSS 1007
    Length:1,007
    Mass (Da):113,554
    Last modified:September 13, 2005 - v1
    Checksum:i4B0E7A79C7BB2DFD
    GO
    Isoform b (identifier: Q45FX5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-32: Missing.

    Show »
    Length:975
    Mass (Da):110,109
    Checksum:iA0A0C4862F56F3B0
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 3232Missing in isoform b. CuratedVSP_018992Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ136172 mRNA. Translation: AAZ66767.1.
    FO080787 Genomic DNA. Translation: CCD66769.1.
    FO080787 Genomic DNA. Translation: CCD66770.1.
    PIRiT15778.
    RefSeqiNP_001041222.1. NM_001047757.2. [Q45FX5-2]
    NP_001041223.1. NM_001047758.1. [Q45FX5-1]
    UniGeneiCel.7082.

    Genome annotation databases

    EnsemblMetazoaiC35B8.2b; C35B8.2b; WBGene00006887. [Q45FX5-1]
    GeneIDi181153.
    KEGGicel:CELE_C35B8.2.
    UCSCiC35B8.2a.1. c. elegans. [Q45FX5-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Nematode tempo - Issue 71 of June 2006

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ136172 mRNA. Translation: AAZ66767.1 .
    FO080787 Genomic DNA. Translation: CCD66769.1 .
    FO080787 Genomic DNA. Translation: CCD66770.1 .
    PIRi T15778.
    RefSeqi NP_001041222.1. NM_001047757.2. [Q45FX5-2 ]
    NP_001041223.1. NM_001047758.1. [Q45FX5-1 ]
    UniGenei Cel.7082.

    3D structure databases

    ProteinModelPortali Q45FX5.
    SMRi Q45FX5. Positions 34-658, 801-990.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 46070. 6 interactions.
    DIPi DIP-25959N.
    IntActi Q45FX5. 27 interactions.
    MINTi MINT-1070973.

    Proteomic databases

    PaxDbi Q45FX5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai C35B8.2b ; C35B8.2b ; WBGene00006887 . [Q45FX5-1 ]
    GeneIDi 181153.
    KEGGi cel:CELE_C35B8.2.
    UCSCi C35B8.2a.1. c. elegans. [Q45FX5-1 ]

    Organism-specific databases

    CTDi 181153.
    WormBasei C35B8.2a ; CE38040 ; WBGene00006887 ; vav-1.
    C35B8.2b ; CE39333 ; WBGene00006887 ; vav-1.

    Phylogenomic databases

    eggNOGi COG5422.
    GeneTreei ENSGT00740000115307.
    HOGENOMi HOG000020846.
    InParanoidi Q45FX5.
    KOi K05730.
    OMAi VELVQYY.
    OrthoDBi EOG78SQH7.
    PhylomeDBi Q45FX5.

    Enzyme and pathway databases

    SignaLinki Q45FX5.

    Miscellaneous databases

    NextBioi 912636.

    Family and domain databases

    Gene3Di 1.10.418.10. 1 hit.
    1.20.900.10. 1 hit.
    2.30.29.30. 1 hit.
    3.30.505.10. 1 hit.
    InterProi IPR001715. CH-domain.
    IPR000219. DH-domain.
    IPR001331. GDS_CDC24_CS.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    IPR000980. SH2.
    IPR011511. SH3_2.
    IPR001452. SH3_domain.
    IPR013315. Spectrin_alpha_SH3.
    [Graphical view ]
    Pfami PF00130. C1_1. 1 hit.
    PF00307. CH. 1 hit.
    PF00169. PH. 1 hit.
    PF00621. RhoGEF. 1 hit.
    PF00017. SH2. 1 hit.
    PF07653. SH3_2. 1 hit.
    [Graphical view ]
    PRINTSi PR00401. SH2DOMAIN.
    PR00452. SH3DOMAIN.
    PR01887. SPECTRNALPHA.
    SMARTi SM00109. C1. 1 hit.
    SM00033. CH. 1 hit.
    SM00233. PH. 1 hit.
    SM00325. RhoGEF. 1 hit.
    SM00252. SH2. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47576. SSF47576. 1 hit.
    SSF48065. SSF48065. 1 hit.
    SSF50044. SSF50044. 2 hits.
    SSF55550. SSF55550. 1 hit.
    PROSITEi PS50021. CH. 1 hit.
    PS00741. DH_1. 1 hit.
    PS50010. DH_2. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS50001. SH2. 1 hit.
    PS50002. SH3. 1 hit.
    PS00479. ZF_DAG_PE_1. 1 hit.
    PS50081. ZF_DAG_PE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Rho/Rac family guanine nucleotide exchange factor VAV-1 regulates rhythmic behaviors in Caenorhabditis elegans."
      Norman K.R., Fazzio R.T., Mellem J.E., Espelt M.V., Strange K., Beckerle M.C., Maricq A.V.
      Cell 123:119-132(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF TYR-183; TYR-200 AND TYR-217.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: Bristol N2.

    Entry informationi

    Entry nameiVAV_CAEEL
    AccessioniPrimary (citable) accession number: Q45FX5
    Secondary accession number(s): Q18479
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2006
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3