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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

dut

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.UniRule annotation

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: dUMP biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes dUMP from dCTP (dUTP route).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. dCTP deaminase (dcd)
  2. Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut)
This subpathway is part of the pathway dUMP biosynthesis, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dUMP from dCTP (dUTP route), the pathway dUMP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei84SubstrateUniRule annotation1
Binding sitei98Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1

GO - Molecular functioni

  • dUTP diphosphatase activity Source: TIGR
  • magnesium ion binding Source: GO_Central

GO - Biological processi

  • 2'-deoxyribonucleotide metabolic process Source: TIGR
  • dUMP biosynthetic process Source: GO_Central
  • dUTP catabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00610; UER00666.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolaseUniRule annotation (EC:3.6.1.23UniRule annotation)
Short name:
dUTPaseUniRule annotation
Alternative name(s):
dUTP pyrophosphataseUniRule annotation
Gene namesi
Name:dutUniRule annotation
Ordered Locus Names:CBU_0293
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
Proteomesi
  • UP000002671 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001828561 – 152Deoxyuridine 5'-triphosphate nucleotidohydrolaseAdd BLAST152

Interactioni

Protein-protein interaction databases

STRINGi227377.CBU_0293.

Structurei

Secondary structure

1152
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 9Combined sources4
Turni15 – 17Combined sources3
Beta strandi29 – 34Combined sources6
Beta strandi41 – 43Combined sources3
Beta strandi48 – 58Combined sources11
Beta strandi64 – 69Combined sources6
Helixi72 – 78Combined sources7
Beta strandi80 – 82Combined sources3
Beta strandi85 – 89Combined sources5
Beta strandi95 – 103Combined sources9
Beta strandi105 – 107Combined sources3
Beta strandi109 – 111Combined sources3
Beta strandi116 – 124Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TQZX-ray1.75A1-152[»]
ProteinModelPortaliQ45920.
SMRiQ45920.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni71 – 73Substrate bindingUniRule annotation3
Regioni88 – 90Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the dUTPase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108Z1K. Bacteria.
COG0756. LUCA.
HOGENOMiHOG000028968.
KOiK01520.
OMAiMVSAWNR.

Family and domain databases

CDDicd07557. trimeric_dUTPase. 1 hit.
Gene3Di2.70.40.10. 1 hit.
HAMAPiMF_00116. dUTPase_bact. 1 hit.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
IPR033704. dUTPase_trimeric.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.

Sequencei

Sequence statusi: Complete.

Q45920-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHSVQLKIL DKRLGSEFPL PAYATTGSAG LDLRACLDEP LKIEPDETCL
60 70 80 90 100
ISTGLAIYLG HSNVAATILP RSGLGHKHGI VLGNLVGLID SDYQGPLMVS
110 120 130 140 150
CWNRGKEPYT INPGDRIAQL VVLPILKAQF AVVEEFELTE RGAGGFGSSG

QN
Length:152
Mass (Da):16,214
Last modified:November 1, 1996 - v1
Checksum:i00041727C1882C57
GO

Sequence cautioni

The sequence AAO89850 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79075 Genomic DNA. Translation: CAA55678.1.
AE016828 Genomic DNA. Translation: AAO89850.2. Different initiation.
PIRiS44300.
RefSeqiNP_819336.2. NC_002971.3.
WP_005771437.1. NZ_CDBG01000001.1.

Genome annotation databases

EnsemblBacteriaiAAO89850; AAO89850; CBU_0293.
GeneIDi1208175.
KEGGicbu:CBU_0293.
PATRICi17929281. VBICoxBur82552_0288.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79075 Genomic DNA. Translation: CAA55678.1.
AE016828 Genomic DNA. Translation: AAO89850.2. Different initiation.
PIRiS44300.
RefSeqiNP_819336.2. NC_002971.3.
WP_005771437.1. NZ_CDBG01000001.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TQZX-ray1.75A1-152[»]
ProteinModelPortaliQ45920.
SMRiQ45920.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi227377.CBU_0293.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO89850; AAO89850; CBU_0293.
GeneIDi1208175.
KEGGicbu:CBU_0293.
PATRICi17929281. VBICoxBur82552_0288.

Phylogenomic databases

eggNOGiENOG4108Z1K. Bacteria.
COG0756. LUCA.
HOGENOMiHOG000028968.
KOiK01520.
OMAiMVSAWNR.

Enzyme and pathway databases

UniPathwayiUPA00610; UER00666.

Family and domain databases

CDDicd07557. trimeric_dUTPase. 1 hit.
Gene3Di2.70.40.10. 1 hit.
HAMAPiMF_00116. dUTPase_bact. 1 hit.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
IPR033704. dUTPase_trimeric.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDUT_COXBU
AccessioniPrimary (citable) accession number: Q45920
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.