Q45885 (DJLA_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DnaJ-like protein DjlA

Alternative name(s):
Mucoidy activation protein MucZ

Gene names

Name:	djlA
Synonyms:	mucZ
Ordered Locus Names:	CBU_1873

Organism

Coxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]

Taxonomic identifier

227377 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella › Coxiella burnetii

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Regulatory DnaK co-chaperone. Direct interaction between DnaK and DjlA is needed for the induction of the wcaABCDE operon, involved in the synthesis of a colanic acid polysaccharide capsule, possibly through activation of the RcsB/RcsC phosphotransfer signaling pathway. The colanic acid capsule may help the bacterium survive conditions outside the host By similarity. HAMAP-Rule MF_01153
Subunit structure	Homodimer Potential.
Subcellular location	Cell inner membrane; Single-pass type III membrane protein HAMAP-Rule MF_01153.
Domain	The transmembrane domain is a dimerization domain By similarity. HAMAP-Rule MF_01153
Sequence similarities	Contains 1 J domain.
Sequence caution	The sequence AAO91364.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Chaperone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 270	270	DnaJ-like protein DjlA HAMAP-Rule MF_01153		PRO_0000209423
Regions
	Topological domain	1 – 6	6	Periplasmic Potential
	Transmembrane	7 – 30	24	Helical; By similarity
	Topological domain	31 – 270	240	Cytoplasmic By similarity
	Domain	204 – 270	67	J

Sequences

	Sequence		Length	Mass (Da)
	Q45885 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: E75DA56B15BF4810	FASTA	270	31,291
	10 20 30 40 50 60 MNWIGKLIGM MLGFILAGPI GLIIGLFIGH VVFDQGRFRQ WFQTTASARS QPSKIQEVFF 70 80 90 100 110 120 NTTFRVMGFV AKADGRVSEN EIRQARQVMQ QMNLDDSMKR EAIRLFTEGK QPNFNLDESL 130 140 150 160 170 180 NELRQACVFQ PALLRVFLEI QIQMASADGQ GLSGQKRQVL QTICRRLEVF GFDYNQFEQR 190 200 210 220 230 240 FRAEQNYQRY QQRATQDPRA YLNDAYKVLG LTSAATDSEI KKSYRRLMSQ HHPDKLMAKG 250 260 270 LPPEMMKMAT QKTQQIKKAY EQIRKVRSMV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a Coxiella burnetti gene product that activates capsule synthesis in Escherichia coli: requirement for the heat shock chaperone DnaK and the two-component regulator RcsC." Zuber M., Hoover T.A., Court D.L. J. Bacteriol. 177:4238-4244(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Nine Mile.
[2]	"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RSA 493 / Nine Mile phase I.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42518 Genomic DNA. Translation: AAA79969.1. AE016828 Genomic DNA. Translation: AAO91364.1. Different initiation.
PIR	I40852.
RefSeq	NP_820850.1. NC_002971.3.
3D structure databases
ProteinModelPortal	Q45885.
ModBase	Search...
Protein-protein interaction databases
STRING	227377.CBU_1873.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO91364; AAO91364; CBU_1873.
GeneID	1209786.
KEGG	cbu:CBU_1873.
PATRIC	17932477. VBICoxBur82552_1855.
Phylogenomic databases
eggNOG	COG1076.
HOGENOM	HOG000272779.
KO	K05801.
OMA	LRACWRM.
ProtClustDB	PRK09430.
Enzyme and pathway databases
BioCyc	CBUR227377:GJ7S-1849-MONOMER.
Family and domain databases
Gene3D	1.10.287.110. 1 hit.
HAMAP	MF_01153. DjlA.
InterPro	IPR023749. DjlA. IPR001623. DnaJ_domain. IPR007791. Heat_shock_DnaJ-like_DjlA. [Graphical view]
Pfam	PF00226. DnaJ. 1 hit. PF05099. TerB. 1 hit. [Graphical view]
PRINTS	PR00625. JDOMAIN.
SMART	SM00271. DnaJ. 1 hit. [Graphical view]
SUPFAM	SSF46565. DnaJ_N. 1 hit.
PROSITE	PS00636. DNAJ_1. False negative. PS50076. DNAJ_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DJLA_COXBU

Accession

Primary (citable) accession number: Q45885

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program