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Protein

Beta-lactamase

Gene

bla

Organism
Bacillus thuringiensis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This protein is a beta-lactamase with a substrate specificity for penicillins.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei92 – 921Acyl-ester intermediatePROSITE-ProRule annotation
Active sitei188 – 1881Proton acceptorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase (EC:3.5.2.6)
Alternative name(s):
Penicillinase
Gene namesi
Name:bla
OrganismiBacillus thuringiensis
Taxonomic identifieri1428 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence AnalysisAdd
BLAST
Chaini29 – 309281Beta-lactamasePRO_0000016976Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ45726.
SMRiQ45726. Positions 55-308.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni254 – 2563Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q45726-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMILKNKRML KIGICVGILG LSITSLEAFT GGALQVEAKQ KTGQVKHKNQ
60 70 80 90 100
ATHKEFSQLE KKFDARVGVY AIDTGTNQTI SYRSNERFAF ASTYKALAAG
110 120 130 140 150
VLLQQNSIDT LNEVITFTKE DLVDYSPVTE KHVDTGMKLG EIAEAAVRSS
160 170 180 190 200
DNTAGNILFN KIGGPKGYEK ALRKMGDRVT MSDRFETELN EAIPGDIRDT
210 220 230 240 250
STAKRIATNL KAFTVGNALP AEKRKILTEW MKGNATGDKL IRAGVPTDWV
260 270 280 290 300
VGDKSGAGSY GTRNDIAIVW PPNRAPIIIA ILSSKDEKEA SYDNQLIAEA

TEVIVKALK
Length:309
Mass (Da):33,699
Last modified:November 1, 1996 - v1
Checksum:i9C10BE11213237C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83424 Genomic DNA. Translation: CAA58448.1.
PIRiJC4117.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83424 Genomic DNA. Translation: CAA58448.1.
PIRiJC4117.

3D structure databases

ProteinModelPortaliQ45726.
SMRiQ45726. Positions 55-308.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and sequencing of a beta-lactamase-encoding gene from the insect pathogen Bacillus thuringiensis."
    Zhang M.-Y., Loevgren A.
    Gene 158:83-86(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Bt13.

Entry informationi

Entry nameiBLAC_BACTU
AccessioniPrimary (citable) accession number: Q45726
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: June 24, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.