Reviewed, UniProtKB/Swiss-Prot Q45726 (BLAC_BACTU)

Names and origin

Protein names

Recommended name:
    Beta-lactamase
    EC=3.5.2.6
Alternative name(s):
    Penicillinase

Gene names

Name:

bla

Organism

Bacillus thuringiensis

Taxonomic identifier

1428 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein is a beta-lactamase with a substrate specificity for penicillins.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 309	281	Beta-lactamase		PRO_0000016976
Regions
	Region	254 – 256	3	Substrate binding By similarity
Sites
	Active site	92	1	Acyl-ester intermediate By similarity
	Active site	188	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q45726-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 9C10BE11213237C1	FASTA	309	33,699
	10 20 30 40 50 60 MMILKNKRML KIGICVGILG LSITSLEAFT GGALQVEAKQ KTGQVKHKNQ ATHKEFSQLE 70 80 90 100 110 120 KKFDARVGVY AIDTGTNQTI SYRSNERFAF ASTYKALAAG VLLQQNSIDT LNEVITFTKE 130 140 150 160 170 180 DLVDYSPVTE KHVDTGMKLG EIAEAAVRSS DNTAGNILFN KIGGPKGYEK ALRKMGDRVT 190 200 210 220 230 240 MSDRFETELN EAIPGDIRDT STAKRIATNL KAFTVGNALP AEKRKILTEW MKGNATGDKL 250 260 270 280 290 300 IRAGVPTDWV VGDKSGAGSY GTRNDIAIVW PPNRAPIIIA ILSSKDEKEA SYDNQLIAEA TEVIVKALK

References

[1]	"Cloning and sequencing of a beta-lactamase-encoding gene from the insect pathogen Bacillus thuringiensis." Zhang M.-Y., Loevgren A. Gene 158:83-86(1995) [PubMed: 7789815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Bt13.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X83424 Genomic DNA. Translation: CAA58448.1.
PIR	JC4117.
3D structure databases
HSSP	HSSP built from PDB template 1I2W based on UniProtKB P00808.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.5.2.6. 141666.
Family and domain databases
InterPro	IPR001466. Beta_lactamase-related. IPR000871. Beta_lactamase_A/D. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLAC_BACTU

Accession

Primary (citable) accession number: Q45726

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)