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Protein
Submitted name:

Microbial serine proteinases

Gene

sub

Organism
Bacillus subtilis
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi139 – 1391Calcium 1Combined sources
Metal bindingi159 – 1591Calcium 1Combined sources
Metal bindingi207 – 2071Calcium 1Combined sources
Metal bindingi323 – 3231Calcium 2; via carbonyl oxygenCombined sources
Metal bindingi326 – 3261Calcium 2Combined sources
Metal bindingi328 – 3281Calcium 2; via carbonyl oxygenCombined sources
Metal bindingi330 – 3301Calcium 2Combined sources
Metal bindingi333 – 3331Calcium 2Combined sources
Metal bindingi396 – 3961Calcium 3Combined sources
Metal bindingi397 – 3971Calcium 3; via carbonyl oxygenCombined sources
Metal bindingi404 – 4041Calcium 3; via carbonyl oxygenCombined sources
Metal bindingi406 – 4061Calcium 3; via carbonyl oxygenCombined sources
Metal bindingi408 – 4081Calcium 3Combined sources

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine proteaseUniRule annotation

Keywords - Ligandi

CalciumCombined sources, Metal-bindingCombined sources

Enzyme and pathway databases

BRENDAi3.4.21.62. 658.

Protein family/group databases

MEROPSiS08.140.

Names & Taxonomyi

Protein namesi
Submitted name:
Microbial serine proteinasesImported (EC:3.4.21.14Imported)
Gene namesi
Name:subImported
OrganismiBacillus subtilisImported
Taxonomic identifieri1423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence analysisAdd
BLAST
Chaini32 – 419388Sequence analysisPRO_5004232008Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi161 ↔ 175Combined sources

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GKOX-ray1.40A111-419[»]
ProteinModelPortaliQ45681.
SMRiQ45681. Positions 111-419.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ45681.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini135 – 406272Peptidase S8InterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S8 family.UniRule annotation

Keywords - Domaini

SignalSequence analysis

Family and domain databases

Gene3Di3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 2 hits.
PfamiPF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q45681-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRSGKIFTT AMLAVTLMMP AIGVSANRGN AADGNEKFRV LVDSANQNNL
60 70 80 90 100
KNVKEQYGVH WDFAGEGFTT NMNEKQFNAL QNNKNLTVEK VPELEIATAT
110 120 130 140 150
NKPEALYNAM AASQSTPWGI KAIYNNSNLT STSGGAGINI AVLDTGVNTN
160 170 180 190 200
HPDLSNNVEQ CKDFTVGTNF TDNSCTDRQG HGTHVAGSAL ANGGTGSGVY
210 220 230 240 250
GVAPEADLWA YKVLGDDGSG YADDIAEAIR HAGDQATALN TKVVINMSLG
260 270 280 290 300
SSGESSLITN AVDYAYDKGV LIIAAAGNSG PKPGSIGYPG ALVNAVAVAA
310 320 330 340 350
LENTIQNGTY RVADFSSRGH KRTAGDYVIQ KGDVEISAPG AAVYSTWFDG
360 370 380 390 400
GYATISGTSM ASPHAAGLAA KIWAQSPAAS NVDVRGELQT RASVNDILSG
410
NSAGSGDDIA SGFGFAKVQ
Length:419
Mass (Da):43,346
Last modified:November 1, 1996 - v1
Checksum:i529D6F785F11CEB8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63533 Genomic DNA. Translation: CAA45096.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63533 Genomic DNA. Translation: CAA45096.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GKOX-ray1.40A111-419[»]
ProteinModelPortaliQ45681.
SMRiQ45681. Positions 111-419.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS08.140.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.4.21.62. 658.

Miscellaneous databases

EvolutionaryTraceiQ45681.

Family and domain databases

Gene3Di3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 2 hits.
PfamiPF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence of the subtilisin-encoding gene from an antarctic psychrotroph Bacillus TA41."
    Davail S., Feller G., Narinx E., Gerday C.
    Gene 119:143-144(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: TA 41Imported.
  2. "The crystal structures of the psychrophilic subtilisin S41 and the mesophilic subtilisin Sph reveal the same calcium-loaded state."
    Almog O., Gonzalez A., Godin N., de Leeuw M., Mekel M.J., Klein D., Braun S., Shoham G., Walter R.L.
    Proteins 74:489-496(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) OF 111-419 IN COMPLEX WITH CALCIUM, DISULFIDE BONDS.

Entry informationi

Entry nameiQ45681_BACIU
AccessioniPrimary (citable) accession number: Q45681
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.