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Protein

Thermophilic serine proteinase

Gene
N/A
Organism
Bacillus sp. (strain AK1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cofactori

Protein has several cofactor binding sites:

pH dependencei

Optimum pH is 8.5.

Temperature dependencei

Optimum temperature is 75 degrees Celsius.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi126Calcium 11 Publication1
Active sitei160Charge relay system1 Publication1
Metal bindingi168Calcium 3; via carbonyl oxygen1 Publication1
Metal bindingi169Calcium 11 Publication1
Metal bindingi171Calcium 31 Publication1
Metal bindingi179Calcium 21 Publication1
Metal bindingi184Calcium 21 Publication1
Metal bindingi186Calcium 2; via carbonyl oxygen1 Publication1
Active sitei193Charge relay system1 Publication1
Metal bindingi204Calcium 11 Publication1
Metal bindingi204Calcium 31 Publication1
Metal bindingi207Calcium 11 Publication1
Metal bindingi209Calcium 1; via carbonyl oxygen1 Publication1
Metal bindingi211Calcium 1; via carbonyl oxygen1 Publication1
Metal bindingi297Sodium; via carbonyl oxygen1 Publication1
Metal bindingi300Sodium; via carbonyl oxygen1 Publication1
Metal bindingi323Sodium1 Publication1
Active sitei347Charge relay system1 Publication1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.21.62. 691.

Protein family/group databases

MEROPSiS08.009.

Names & Taxonomyi

Protein namesi
Recommended name:
Thermophilic serine proteinase (EC:3.4.21.-)
Alternative name(s):
Ak.1 protease
OrganismiBacillus sp. (strain AK1)
Taxonomic identifieri268807 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000002719325 – 121Add BLAST97
ChainiPRO_0000027194122 – 401Thermophilic serine proteinaseAdd BLAST280

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi258 ↔ 260

Keywords - PTMi

Disulfide bond, Zymogen

Structurei

Secondary structure

1401
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi129 – 132Combined sources4
Helixi137 – 139Combined sources3
Helixi141 – 144Combined sources4
Turni145 – 147Combined sources3
Beta strandi155 – 161Combined sources7
Turni168 – 173Combined sources6
Beta strandi174 – 179Combined sources6
Turni180 – 183Combined sources4
Beta strandi190 – 192Combined sources3
Helixi193 – 202Combined sources10
Beta strandi206 – 210Combined sources5
Beta strandi214 – 217Combined sources4
Beta strandi219 – 224Combined sources6
Helixi234 – 246Combined sources13
Beta strandi250 – 254Combined sources5
Helixi263 – 274Combined sources12
Beta strandi278 – 282Combined sources5
Beta strandi300 – 306Combined sources7
Beta strandi324 – 327Combined sources4
Beta strandi329 – 335Combined sources7
Turni336 – 338Combined sources3
Beta strandi339 – 343Combined sources5
Helixi346 – 362Combined sources17
Helixi367 – 376Combined sources10
Turni382 – 386Combined sources5
Beta strandi387 – 392Combined sources6
Helixi395 – 399Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DBIX-ray1.80A122-401[»]
ProteinModelPortaliQ45670.
SMRiQ45670.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ45670.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini156 – 397Peptidase S8Add BLAST242

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated
Contains 1 peptidase S8 domain.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.30.70.80. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR010259. S8pro/Inhibitor_I9.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 2 hits.
PfamiPF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q45670-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFKAIVSLS LAVSMSLFPF LVEAASNDGV ESPKTVSEIN VSHEKGAYVQ
60 70 80 90 100
GEVIVQFKEQ VNAEEKAKAL KEVGATAVPD NDRVKSKFNV LKVGNVEAVV
110 120 130 140 150
KALNNNPLVE YAEPNYLFNA AWTPNDTYYQ GYQYGPQNTY TDYAWDVTKG
160 170 180 190 200
SSGQEIAVID TGVDYTHPDL DGKVIKGYDF VDNDYDPMDL NNHGTHVAGI
210 220 230 240 250
AAAETNNATG IAGMAPNTRI LAVRALDRNG SGTLSDIADA IIYAADSGAE
260 270 280 290 300
VINLSLGCDC HTTTLENAVN YAWNKGSVVV AAAGNNGSST TFEPASYENV
310 320 330 340 350
IAVGAVDQYD RLASFSNYGT WVDVVAPGVD IVSTITGNRY AYMSGTSMAS
360 370 380 390 400
PHVAGLAALL ASQGRNNIEI RQAIEQTADK ISGTGTYFKY GRINSYNAVT

Y
Length:401
Mass (Da):42,835
Last modified:November 1, 1996 - v1
Checksum:i1C736EF4A89F256F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29506 Genomic DNA. Translation: AAA63688.1.
PIRiI39974.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29506 Genomic DNA. Translation: AAA63688.1.
PIRiI39974.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DBIX-ray1.80A122-401[»]
ProteinModelPortaliQ45670.
SMRiQ45670.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS08.009.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.4.21.62. 691.

Miscellaneous databases

EvolutionaryTraceiQ45670.

Family and domain databases

Gene3Di3.30.70.80. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR010259. S8pro/Inhibitor_I9.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 2 hits.
PfamiPF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHES_BACSJ
AccessioniPrimary (citable) accession number: Q45670
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.