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Protein

Thermophilic serine proteinase

Gene
N/A
Organism
Bacillus sp. (strain AK1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cofactori

Protein has several cofactor binding sites:

pH dependencei

Optimum pH is 8.5.

Temperature dependencei

Optimum temperature is 75 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi126 – 1261Calcium 11 Publication
Active sitei160 – 1601Charge relay system1 Publication
Metal bindingi168 – 1681Calcium 3; via carbonyl oxygen1 Publication
Metal bindingi169 – 1691Calcium 11 Publication
Metal bindingi171 – 1711Calcium 31 Publication
Metal bindingi179 – 1791Calcium 21 Publication
Metal bindingi184 – 1841Calcium 21 Publication
Metal bindingi186 – 1861Calcium 2; via carbonyl oxygen1 Publication
Active sitei193 – 1931Charge relay system1 Publication
Metal bindingi204 – 2041Calcium 11 Publication
Metal bindingi204 – 2041Calcium 31 Publication
Metal bindingi207 – 2071Calcium 11 Publication
Metal bindingi209 – 2091Calcium 1; via carbonyl oxygen1 Publication
Metal bindingi211 – 2111Calcium 1; via carbonyl oxygen1 Publication
Metal bindingi297 – 2971Sodium; via carbonyl oxygen1 Publication
Metal bindingi300 – 3001Sodium; via carbonyl oxygen1 Publication
Metal bindingi323 – 3231Sodium1 Publication
Active sitei347 – 3471Charge relay system1 Publication

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.21.62. 691.

Protein family/group databases

MEROPSiS08.009.

Names & Taxonomyi

Protein namesi
Recommended name:
Thermophilic serine proteinase (EC:3.4.21.-)
Alternative name(s):
Ak.1 protease
OrganismiBacillus sp. (strain AK1)
Taxonomic identifieri268807 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Propeptidei25 – 12197PRO_0000027193Add
BLAST
Chaini122 – 401280Thermophilic serine proteinasePRO_0000027194Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi258 ↔ 260

Keywords - PTMi

Disulfide bond, Zymogen

Structurei

Secondary structure

1
401
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi129 – 1324Combined sources
Helixi137 – 1393Combined sources
Helixi141 – 1444Combined sources
Turni145 – 1473Combined sources
Beta strandi155 – 1617Combined sources
Turni168 – 1736Combined sources
Beta strandi174 – 1796Combined sources
Turni180 – 1834Combined sources
Beta strandi190 – 1923Combined sources
Helixi193 – 20210Combined sources
Beta strandi206 – 2105Combined sources
Beta strandi214 – 2174Combined sources
Beta strandi219 – 2246Combined sources
Helixi234 – 24613Combined sources
Beta strandi250 – 2545Combined sources
Helixi263 – 27412Combined sources
Beta strandi278 – 2825Combined sources
Beta strandi300 – 3067Combined sources
Beta strandi324 – 3274Combined sources
Beta strandi329 – 3357Combined sources
Turni336 – 3383Combined sources
Beta strandi339 – 3435Combined sources
Helixi346 – 36217Combined sources
Helixi367 – 37610Combined sources
Turni382 – 3865Combined sources
Beta strandi387 – 3926Combined sources
Helixi395 – 3995Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DBIX-ray1.80A122-401[»]
ProteinModelPortaliQ45670.
SMRiQ45670. Positions 122-401.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ45670.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini156 – 397242Peptidase S8Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated
Contains 1 peptidase S8 domain.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.30.70.80. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR010259. S8pro/Inhibitor_I9.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 2 hits.
PfamiPF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q45670-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFKAIVSLS LAVSMSLFPF LVEAASNDGV ESPKTVSEIN VSHEKGAYVQ
60 70 80 90 100
GEVIVQFKEQ VNAEEKAKAL KEVGATAVPD NDRVKSKFNV LKVGNVEAVV
110 120 130 140 150
KALNNNPLVE YAEPNYLFNA AWTPNDTYYQ GYQYGPQNTY TDYAWDVTKG
160 170 180 190 200
SSGQEIAVID TGVDYTHPDL DGKVIKGYDF VDNDYDPMDL NNHGTHVAGI
210 220 230 240 250
AAAETNNATG IAGMAPNTRI LAVRALDRNG SGTLSDIADA IIYAADSGAE
260 270 280 290 300
VINLSLGCDC HTTTLENAVN YAWNKGSVVV AAAGNNGSST TFEPASYENV
310 320 330 340 350
IAVGAVDQYD RLASFSNYGT WVDVVAPGVD IVSTITGNRY AYMSGTSMAS
360 370 380 390 400
PHVAGLAALL ASQGRNNIEI RQAIEQTADK ISGTGTYFKY GRINSYNAVT

Y
Length:401
Mass (Da):42,835
Last modified:November 1, 1996 - v1
Checksum:i1C736EF4A89F256F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29506 Genomic DNA. Translation: AAA63688.1.
PIRiI39974.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29506 Genomic DNA. Translation: AAA63688.1.
PIRiI39974.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DBIX-ray1.80A122-401[»]
ProteinModelPortaliQ45670.
SMRiQ45670. Positions 122-401.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS08.009.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.4.21.62. 691.

Miscellaneous databases

EvolutionaryTraceiQ45670.

Family and domain databases

Gene3Di3.30.70.80. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR010259. S8pro/Inhibitor_I9.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 2 hits.
PfamiPF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHES_BACSJ
AccessioniPrimary (citable) accession number: Q45670
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: November 11, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.