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Protein

Sensor histidine kinase WalK

Gene

walK

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system WalK/WalR involved in the regulation of the ftsAZ operon, the yocH and ykvT, cwlO, lytE, ydjM, yjeA, yoeB genes and the tagAB and tagDEF operons. Phosphorylates WalR.5 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.1 Publication

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU40400-MONOMER.
BRENDAi2.7.13.3. 658.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor histidine kinase WalKCurated (EC:2.7.13.31 Publication)
Gene namesi
Name:walKBy similarityImported
Synonyms:yycG
Ordered Locus Names:BSU40400
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 13CytoplasmicSequence analysisAdd BLAST13
Transmembranei14 – 34HelicalSequence analysisAdd BLAST21
Topological domaini35 – 182ExtracellularSequence analysisAdd BLAST148
Transmembranei183 – 203HelicalSequence analysisAdd BLAST21
Topological domaini204 – 611CytoplasmicSequence analysisAdd BLAST408

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi386H → R or A: Loss of phosphorylation. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL1075059.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000749181 – 611Sensor histidine kinase WalKAdd BLAST611

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei386Phosphohistidine; by autocatalysis1 Publication1

Post-translational modificationi

Autophosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ45614.
PRIDEiQ45614.

Expressioni

Developmental stagei

Expressed during exponential growth and shut down at the entry into stationary phase.

Interactioni

Subunit structurei

Homodimer. Interacts with YycH and YycI.1 Publication

Protein-protein interaction databases

IntActiQ45614. 2 interactors.
STRINGi224308.Bsubs1_010100021801.

Chemistry databases

BindingDBiQ45614.

Structurei

Secondary structure

1611
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi451 – 453Combined sources3
Helixi454 – 466Combined sources13
Beta strandi475 – 478Combined sources4
Beta strandi485 – 488Combined sources4
Helixi490 – 506Combined sources17
Beta strandi514 – 521Combined sources8
Turni522 – 525Combined sources4
Beta strandi526 – 532Combined sources7
Turni540 – 547Combined sources8
Helixi567 – 577Combined sources11
Beta strandi582 – 587Combined sources6
Turni588 – 590Combined sources3
Beta strandi591 – 601Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SL2X-ray1.61A451-611[»]
ProteinModelPortaliQ45614.
SMRiQ45614.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini204 – 256HAMPPROSITE-ProRule annotationAdd BLAST53
Domaini263 – 324PASCuratedAdd BLAST62
Domaini325 – 379PACPROSITE-ProRule annotationAdd BLAST55
Domaini383 – 602Histidine kinasePROSITE-ProRule annotationAdd BLAST220

Sequence similaritiesi

Contains 1 HAMP domain.PROSITE-ProRule annotation
Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 PAC (PAS-associated C-terminal) domain.PROSITE-ProRule annotation
Contains 1 PAS (PER-ARNT-SIM) domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000223176.
InParanoidiQ45614.
KOiK07652.
OMAiLNEGAWK.
PhylomeDBiQ45614.

Family and domain databases

CDDicd06225. HAMP. 1 hit.
Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
PS50113. PAC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q45614-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKVGFFRSI QFKITLIYVL LIIIAMQIIG VYFVNQVEKS LISSYEQSLN
60 70 80 90 100
QRIDNLSYYI EQEYKSDNDS TVIKDDVSRI LNDFTKSDEV REISFVDKSY
110 120 130 140 150
EVVGSSKPYG EEVAGKQTTD LIFKRIFSTK QSYLRKYYDP KSKIRVLISA
160 170 180 190 200
KPVMTENQEV VGAIYVVASM EDVFNQMKTI NTILASGTGL ALVLTALLGI
210 220 230 240 250
FLARTITHPL SDMRKQAMEL AKGNFSRKVK KYGHDEIGQL ATTFNHLTRE
260 270 280 290 300
LEDAQAMTEG ERRKLASVIA YMTDGVIATN RNGAIILLNS PALELLNVSR
310 320 330 340 350
ETALEMPITS LLGLQENYTF EDLVEQQDSM LLEIERDDEL TVLRVNFSVI
360 370 380 390 400
QREHGKIDGL IAVIYDVTEQ EKMDQERREF VANVSHELRT PLTTMRSYLE
410 420 430 440 450
ALAEGAWENK DIAPRFLMVT QNETERMIRL VNDLLQLSKF DSKDYQFNRE
460 470 480 490 500
WIQIVRFMSL IIDRFEMTKE QHVEFIRNLP DRDLYVEIDQ DKITQVLDNI
510 520 530 540 550
ISNALKYSPE GGHVTFSIDV NEEEELLYIS VKDEGIGIPK KDVEKVFDRF
560 570 580 590 600
YRVDKARTRK LGGTGLGLAI AKEMVQAHGG DIWADSIEGK GTTITFTLPY
610
KEEQEDDWDE A
Length:611
Mass (Da):70,034
Last modified:November 1, 1996 - v1
Checksum:i2E7B7CB538410AE7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78193 Genomic DNA. Translation: BAA11299.1.
AL009126 Genomic DNA. Translation: CAB16077.1.
PIRiF70089.
RefSeqiNP_391920.1. NC_000964.3.
WP_009968432.1. NZ_JNCM01000034.1.

Genome annotation databases

EnsemblBacteriaiCAB16077; CAB16077; BSU40400.
GeneIDi937793.
KEGGibsu:BSU40400.
PATRICi18980158. VBIBacSub10457_4241.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78193 Genomic DNA. Translation: BAA11299.1.
AL009126 Genomic DNA. Translation: CAB16077.1.
PIRiF70089.
RefSeqiNP_391920.1. NC_000964.3.
WP_009968432.1. NZ_JNCM01000034.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SL2X-ray1.61A451-611[»]
ProteinModelPortaliQ45614.
SMRiQ45614.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ45614. 2 interactors.
STRINGi224308.Bsubs1_010100021801.

Chemistry databases

BindingDBiQ45614.
ChEMBLiCHEMBL1075059.

Proteomic databases

PaxDbiQ45614.
PRIDEiQ45614.

Protocols and materials databases

DNASUi937793.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB16077; CAB16077; BSU40400.
GeneIDi937793.
KEGGibsu:BSU40400.
PATRICi18980158. VBIBacSub10457_4241.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000223176.
InParanoidiQ45614.
KOiK07652.
OMAiLNEGAWK.
PhylomeDBiQ45614.

Enzyme and pathway databases

BioCyciBSUB:BSU40400-MONOMER.
BRENDAi2.7.13.3. 658.

Miscellaneous databases

PROiQ45614.

Family and domain databases

CDDicd06225. HAMP. 1 hit.
Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
PS50113. PAC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWALK_BACSU
AccessioniPrimary (citable) accession number: Q45614
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The imidazole derivatives NH125, NH126 and NH127 inhibited the incorporation of phosphate from ATP at 50 µg/ml. The zerumbone derivative NH0891 inhibited WalK.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.