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Protein

Cyclic di-AMP synthase CdaA

Gene

cdaA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

One of 3 paralogous diadenylate cyclases (DAC) in this bacteria, catalyzing the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP) (Probable). Upon expression in E.coli leads to c-di-AMP synthesis (PubMed:23192352). Probably the main producer of c-di-AMP for the cell; is probably implicated in control of peptidogylcan synthesis (PubMed:22211522, PubMed:23192352, PubMed:26240071). In B.subtilis c-di-AMP is a second messenger that mediates growth, DNA repair and cell wall homeostasis; it is toxic when present in excess (PubMed:26240071).1 Publication2 Publications

Catalytic activityi

2 ATP = 2 diphosphate + cyclic di-3',5'-adenylate.UniRule annotation

Enzyme regulationi

DAC activity is stimulated about 20-fold in E.coli by coexpression with CdaR (PubMed:23192352).1 Publication

GO - Molecular functioni

  • adenylate cyclase activity Source: UniProtKB
  • ATP binding Source: UniProtKB-KW
  • nucleotidyltransferase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU01750-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic di-AMP synthase CdaA2 Publications (EC:2.7.7.85UniRule annotation)
Short name:
c-di-AMP synthase
Alternative name(s):
Diadenylate cyclase
Short name:
DACUniRule annotation
Gene namesi
Name:cdaA1 Publication
Synonyms:ybbP
Ordered Locus Names:BSU01750
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation1 Publication; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei12 – 3221HelicalUniRule annotationAdd
BLAST
Transmembranei40 – 6021HelicalUniRule annotationAdd
BLAST
Transmembranei61 – 8121HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Increased sensitivity to the beta-lactam antibiotic cefuroxime (CEF), upon overexpression of the c-di-AMP phosphodiesterase GdpP greatly increased sensitivity to CEF (PubMed:22211522). Double disA-cdaA mutants cannot be made, suggesting they are lethal, while double disA-cdaS and cdaA-cdaS mutants are viable (PubMed:22211522, PubMed:23192352). Depletion of cdaA in double disA-cdaA deletion cells leads to cell lysis (PubMed:22211522). Exponentially growing cells are extremely sensitive to H2O2, no change in response to methyl methanesulfonate (PubMed:25616256).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 273273Cyclic di-AMP synthase CdaAPRO_0000360441Add
BLAST

Proteomic databases

PaxDbiQ45589.

Expressioni

Inductioni

Constitutively expressed, part of the cdaA-cdaR-glmM-glmS operon (PubMed:23192352).1 Publication

Interactioni

Subunit structurei

Probably a homodimer (By similarity). Interacts with CdaR (PubMed:23192352, PubMed:26240071). May interact with GlmM (PubMed:26240071).UniRule annotation2 Publications

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100000998.

Structurei

3D structure databases

ProteinModelPortaliQ45589.
SMRiQ45589. Positions 60-238.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini82 – 242161DACPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the adenylate cyclase family. DacA/CdaA subfamily.UniRule annotation
Contains 1 DAC domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C8B. Bacteria.
COG1624. LUCA.
HOGENOMiHOG000054800.
InParanoidiQ45589.
KOiK18672.
OMAiWRDLLDI.
PhylomeDBiQ45589.

Family and domain databases

HAMAPiMF_01499. DacA. 1 hit.
InterProiIPR014046. c-di-AMP_synthase.
IPR003390. DNA_integrity_scan_DisA_N.
[Graphical view]
PfamiPF02457. DisA_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004793. UCP004793. 1 hit.
TIGRFAMsiTIGR00159. TIGR00159. 1 hit.
PROSITEiPS51794. DAC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q45589-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFEDIPFLQ YLGNAVDILL VWYVIYKLIM VIRGTKAVQL LKGIVVIVLV
60 70 80 90 100
RMASQYLGLS TLQWLMDQAI TWGFLAIIII FQPELRRALE QLGRGRFFSR
110 120 130 140 150
SGTPVEEAQQ KTIEAITKAI NYMAKRRIGA LLTIERDTGM GDYIETGIPL
160 170 180 190 200
NAKVSSELLI NIFIPNTPLH DGAVIMKNNE IAAAACYLPL SESPFISKEL
210 220 230 240 250
GTRHRAAVGI SEVTDSLTII VSEETGGVSV AKNGDLHREL TEEALKEMLE
260 270
AEFKKNTRDT SSNRWYWRGK KNG
Length:273
Mass (Da):30,547
Last modified:June 16, 2009 - v3
Checksum:i29F2984C5F90007C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti270 – 2701K → R in BAA19509 (PubMed:9274029).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB002150 Genomic DNA. Translation: BAA19509.1.
AL009126 Genomic DNA. Translation: CAB11951.2.
PIRiH69744.
RefSeqiNP_388056.2. NC_000964.3.
WP_003223651.1. NZ_JNCM01000030.1.

Genome annotation databases

EnsemblBacteriaiCAB11951; CAB11951; BSU01750.
GeneIDi11238083.
938735.
KEGGibsu:BSU01750.
PATRICi18971903. VBIBacSub10457_0180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB002150 Genomic DNA. Translation: BAA19509.1.
AL009126 Genomic DNA. Translation: CAB11951.2.
PIRiH69744.
RefSeqiNP_388056.2. NC_000964.3.
WP_003223651.1. NZ_JNCM01000030.1.

3D structure databases

ProteinModelPortaliQ45589.
SMRiQ45589. Positions 60-238.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100000998.

Proteomic databases

PaxDbiQ45589.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB11951; CAB11951; BSU01750.
GeneIDi11238083.
938735.
KEGGibsu:BSU01750.
PATRICi18971903. VBIBacSub10457_0180.

Phylogenomic databases

eggNOGiENOG4105C8B. Bacteria.
COG1624. LUCA.
HOGENOMiHOG000054800.
InParanoidiQ45589.
KOiK18672.
OMAiWRDLLDI.
PhylomeDBiQ45589.

Enzyme and pathway databases

BioCyciBSUB:BSU01750-MONOMER.

Family and domain databases

HAMAPiMF_01499. DacA. 1 hit.
InterProiIPR014046. c-di-AMP_synthase.
IPR003390. DNA_integrity_scan_DisA_N.
[Graphical view]
PfamiPF02457. DisA_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004793. UCP004793. 1 hit.
TIGRFAMsiTIGR00159. TIGR00159. 1 hit.
PROSITEiPS51794. DAC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDAA_BACSU
AccessioniPrimary (citable) accession number: Q45589
Secondary accession number(s): Q45590, Q7DL98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: June 16, 2009
Last modified: September 7, 2016
This is version 93 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.