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Q45585 (SIGW_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA polymerase sigma factor SigW
Alternative name(s):
Sigma-W factor
Gene names
Name:sigW
Synonyms:ybbL
Ordered Locus Names:BSU01730
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus ›

Protein attributes

Sequence length187 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification. Ref.5

Enzyme regulation

Is activated by proteolytic destruction of the membrane-bound anti-sigma-W factor RsiW in response to antimicrobial peptides and other agents that damage the cell envelope.

Induction

By different stresses causing damage to the cell envelope, such as alkaline shock, salt shock, phage infection and certain antibiotics that affect cell wall biosynthesis. Ref.3 Ref.4 Ref.6 Ref.7

Sequence similarities

Belongs to the sigma-70 factor family. ECF subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 187187RNA polymerase sigma factor SigW
PRO_0000094016

Regions

DNA binding155 – 17420H-T-H motif By similarity
Motif47 – 6014Polymerase core binding Potential

Sequences

Sequence LengthMass (Da)Tools
Q45585 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 5F532568D462181A

FASTA18721,713
        10         20         30         40         50         60 
MEMMIKKRIK QVKKGDQDAF ADIVDIYKDK IYQLCYRMLG NVHEAEDIAQ EAFIRAYVNI 

        70         80         90        100        110        120 
DSFDINRKFS TWLYRIATNL TIDRIRKKKP DYYLDAEVAG TEGLTMYSQI VADGVLPEDA 

       130        140        150        160        170        180 
VVSLELSNTI QQKILKLPDK YRTVIVLKYI DELSLIEIGE ILNIPVGTVK TRIHRGREAL 


RKQLRDL

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of a 31 kb segment of the Bacillus subtilis chromosome in the area of the rrnH and rrnG operons."
Liu H., Haga K., Yasumoto K., Ohashi Y., Yoshikawa H., Takahashi H.
Microbiology 143:2763-2767(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"Global analysis of the general stress response of Bacillus subtilis."
Petersohn A., Brigulla M., Haas S., Hoheisel J.D., Voelker U., Hecker M.
J. Bacteriol. 183:5617-5631(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[4]"Alkaline shock induces the Bacillus subtilis sigma(W) regulon."
Wiegert T., Homuth G., Versteeg S., Schumann W.
Mol. Microbiol. 41:59-71(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[5]"The extracytoplasmic function (ECF) sigma factors."
Helmann J.D.
Adv. Microb. Physiol. 46:47-110(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Antibiotics that inhibit cell wall biosynthesis induce expression of the Bacillus subtilis sigma(W) and sigma(M) regulons."
Cao M., Wang T., Ye R., Helmann J.D.
Mol. Microbiol. 45:1267-1276(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[7]"Cationic antimicrobial peptides elicit a complex stress response in Bacillus subtilis that involves ECF-type sigma factors and two-component signal transduction systems."
Pietiaeinen M., Gardemeister M., Mecklin M., Leskelae S., Sarvas M., Kontinen V.P.
Microbiology 151:1577-1592(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB002150 Genomic DNA. Translation: BAA19507.1.
AL009126 Genomic DNA. Translation: CAB11949.1.
PIRH69706.
RefSeqNP_388054.1. NC_000964.3.

3D structure databases

ProteinModelPortalQ45585.
SMRQ45585. Positions 3-186.
ModBaseSearch...

Protein-protein interaction databases

IntActQ45585. 1 interaction.
STRING224308.BSU01730.

Proteomic databases

PaxDbQ45585.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB11949; CAB11949; BSU01730.
GeneID938868.
KEGGbsu:BSU01730.
PATRIC18971899. VBIBacSub10457_0178.

Organism-specific databases

GenoListBSU01730. [Micado]

Phylogenomic databases

eggNOGCOG1595.
HOGENOMHOG000094755.
KOK03088.
OMAIRKKKPD.
ProtClustDBPRK09641.

Enzyme and pathway databases

BioCycBSUB:BSU01730-MONOMER.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR014284. RNA_pol_sigma-70_dom.
IPR014294. RNA_pol_sigma-W_bacilli.
IPR000838. RNA_pol_sigma70_ECF_CS.
IPR007627. RNA_pol_sigma70_r2.
IPR013249. RNA_pol_sigma70_r4_t2.
IPR013325. RNA_pol_sigma_r2.
IPR013324. RNA_pol_sigma_r3_r4.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF04542. Sigma70_r2. 1 hit.
PF08281. Sigma70_r4_2. 1 hit.
[Graphical view]
SUPFAMSSF88946. Sigma_r2. 1 hit.
SSF88659. Sigma_r3_r4. 1 hit.
TIGRFAMsTIGR02937. sigma70-ECF. 1 hit.
TIGR02948. SigW_bacill. 1 hit.
PROSITEPS01063. SIGMA70_ECF. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSIGW_BACSU
AccessionPrimary (citable) accession number: Q45585
Secondary accession number(s): O08074
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

SIMILARITY comments

Index of protein domains and families

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