Reviewed, UniProtKB/Swiss-Prot Q45560 (FER_BACSC)

Names and origin

Protein names

Recommended name:
    Ferredoxin 7Fe
Alternative name(s):
    Seven-iron ferredoxin

Gene names

Name:

fdxA

Organism

Bacillus schlegelii

Taxonomic identifier

1484 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	78 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Cofactor	Binds 1 4Fe-4S cluster. Binds 1 3Fe-4S cluster.
Subunit structure	Monomer.
Sequence similarities	Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Repeat
Ligand	3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Technical term	3D-structure
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 78

77

Ferredoxin 7Fe

PRO_0000159172

Regions

Domain

2 – 29

28

4Fe-4S ferredoxin-type 1

Domain

31 – 60

30

4Fe-4S ferredoxin-type 2

Sites

Metal binding

9

1

Iron-sulfur 1 (3Fe-4S)

Metal binding

17

1

Iron-sulfur 1 (3Fe-4S)

Metal binding

21

1

Iron-sulfur 2 (4Fe-4S)

Metal binding

40

1

Iron-sulfur 2 (4Fe-4S)

Metal binding

43

1

Iron-sulfur 2 (4Fe-4S)

Metal binding

46

1

Iron-sulfur 2 (4Fe-4S)

Metal binding

50

1

Iron-sulfur 1 (3Fe-4S)

Experimental info

Mutagenesis

14

1

D → C: Transformation from a 7S to 8S ferredoxin. Ref.4

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

78

Helix Strand Turn

Details...

Turn

8 – 11

4

Turn

18 – 20

3

Beta strand

26 – 28

3

Beta strand

30 – 35

6

Turn

37 – 39

3

Helix

46 – 49

4

Helix

51 – 53

3

Beta strand

54 – 57

4

Turn

58 – 60

3

Helix

63 – 75

13

Sequences

	Sequence		Length	Mass (Da)
	Q45560-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 3295879B2E8C2BAA	FASTA	78	8,874
	10 20 30 40 50 60 MAYVITEPCI GTKDASCVEV CPVDCIHEGE DQYYIDPDVC IDCGACEAVC PVSAIYHEDF 70 VPEEWKSYIQ KNRDFFKK

References

[1]	"Cloning and expression of the gene encoding the 7Fe type ferredoxin from a thermophilic hydrogen oxidizing bacterium, Bacillus schlegelii." Aono S., Nakamura S., Aono R., Okura I. Biochem. Biophys. Res. Commun. 201:938-942(1994) [PubMed: 8003034] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 43741 / DSM 2000 / MA-48.
[2]	"Purification and characterization of a 7Fe ferredoxin from a thermophilic hydrogen-oxidizing bacterium, Bacillus schlegelii." Aono S., Kurita H., Uno S., Okura I. J. Biochem. 112:792-795(1992) [PubMed: 1338329] [Abstract] Cited for: CHARACTERIZATION.
[3]	"Solution structure of the oxidized Fe7S8 ferredoxin from the thermophilic bacterium Bacillus schlegelii by 1H NMR spectroscopy." Aono S., Bentrop D., Bertini I., Donaire A., Luchinat C., Niikura Y., Rosato A. Biochemistry 37:9812-9826(1998) [PubMed: 9657695] [Abstract] Cited for: STRUCTURE BY NMR. Strain: ATCC 43741 / DSM 2000 / MA-48.
[4]	"The D13C variant of Bacillus schlegelii 7Fe ferredoxin is an 8Fe ferredoxin as revealed by 1H-NMR spectroscopy." Aono S., Bentrop D., Bertini I., Luchinat C., Macinai R. FEBS Lett. 412:501-505(1997) [PubMed: 9276454] [Abstract] Cited for: MUTAGENESIS OF ASP-14.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

D29804 Genomic DNA. Translation: BAA06187.1.

PIR

JC2496.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BC6	NMR	-	A	2-78	[»]
1BD6	NMR	-	A	2-78	[»]
1BQX	NMR	-	A	2-77	[»]
1BWE	NMR	-	A	2-78	[»]

ModBase

Search...

Family and domain databases

InterPro

IPR017896. 4Fe4S_Fe-S-bd.
IPR001450. 4Fe4S_Fe_S_bd_subgr.
IPR017900. 4Fe4S_Fe_S_CS.
IPR000813. 7Fe_ferredoxin.
[Graphical view]

Pfam

PF00037. Fer4. 1 hit.
[Graphical view]

PRINTS

PR00354. 7FE8SFRDOXIN.

PROSITE

PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

FER_BACSC

Accession

Primary (citable) accession number: Q45560

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)