ID   TRUA_BACSV              Reviewed;         244 AA.
AC   Q45557;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   02-SEP-2008, entry version 41.
DE   RecName: Full=tRNA pseudouridine synthase A;
DE            EC=5.4.99.12;
DE   AltName: Full=tRNA-uridine isomerase I;
DE   AltName: Full=tRNA pseudouridylate synthase I;
GN   Name=truA;
OS   Bacillus sp. (strain KSM-64).
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=86039;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1368251;
RA   Sumitomo N., Ozaki K., Kawai S., Ito S.;
RT   "Nucleotide sequence of the gene for an alkaline endoglucanase from an
RT   alkalophilic Bacillus and its expression in Escherichia coli and
RT   Bacillus subtilis.";
RL   Biosci. Biotechnol. Biochem. 56:872-877(1992).
CC   -!- FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in
CC       the anticodon stem and loop of transfer RNAs (By similarity).
CC   -!- CATALYTIC ACTIVITY: tRNA uridine = tRNA pseudouridine.
CC   -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase truA
CC       family.
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DR   EMBL; M84963; AAA73188.1; -; Genomic_DNA.
DR   PIR; JT0612; JT0612.
DR   HSSP; P07649; 1DJ0.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IEA:HAMAP.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:HAMAP.
DR   HAMAP; MF_00171; -; 1.
DR   InterPro; IPR001406; tRNA_psdUrid_synth.
DR   Gene3D; G3DSA:3.30.70.580; PseudoU_synth_1; 1.
DR   Gene3D; G3DSA:3.30.70.660; PseudoU_synth_1; 1.
DR   PANTHER; PTHR11142; PseudoU_synth_1; 1.
DR   Pfam; PF01416; PseudoU_synth_1; 2.
DR   PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1.
DR   TIGRFAMs; TIGR00071; hisT_truA; 1.
PE   3: Inferred from homology;
KW   Isomerase; tRNA processing.
FT   CHAIN         1    244       tRNA pseudouridine synthase A.
FT                                /FTId=PRO_0000057333.
FT   ACT_SITE     53     53       Nucleophile (By similarity).
SQ   SEQUENCE   244 AA;  27866 MW;  2D2BF29B1CCB3B89 CRC64;
     MNNYKLMIQY DGGRYKGWQR LGNGENTIQG KIETVLSEMV GRKIEIIGSG RTDAGVHALG
     QVANVKLSEN FTVKEVKEYL NRYLPHDISV TEVTLVPDRF HSRYNAKDKT YLYKIWNEDY
     THPFMRKYSL HIEKKLHIDN MVKASQLFVG EHDFTAFSNA KSKKKTNTRT IHSITIQDNQ
     GFIDIRVCGD GFLYNMVRKM VGTLIEVGLG EKEPEQVLTI LESKDRSQAG FADATGLYLE
     GISF
//