Q45557 (TRUA_BACSV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA pseudouridine synthase A
EC=5.4.99.12

Alternative name(s):
tRNA pseudouridine(38-40) synthase
tRNA pseudouridylate synthase I
tRNA-uridine isomerase I

Gene names

Name:

truA

Organism

Bacillus sp. (strain KSM-64)

Taxonomic identifier

86039 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity. HAMAP MF_00171
Catalytic activity	tRNA uridine(38-40) = tRNA pseudouridine(38-40). HAMAP MF_00171
Subunit structure	Homodimer By similarity. HAMAP MF_00171
Sequence similarities	Belongs to the tRNA pseudouridine synthase TruA family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Isomerase
Gene Ontology (GO)
Biological process	pseudouridine synthesis Inferred from electronic annotation. Source: InterPro tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro pseudouridine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	tRNA pseudouridine synthase A HAMAP MF_00171		PRO_0000057333
Sites
	Active site	53	1	Nucleophile By similarity
	Binding site	111	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q45557 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 2D2BF29B1CCB3B89	FASTA	244	27,866
	10 20 30 40 50 60 MNNYKLMIQY DGGRYKGWQR LGNGENTIQG KIETVLSEMV GRKIEIIGSG RTDAGVHALG 70 80 90 100 110 120 QVANVKLSEN FTVKEVKEYL NRYLPHDISV TEVTLVPDRF HSRYNAKDKT YLYKIWNEDY 130 140 150 160 170 180 THPFMRKYSL HIEKKLHIDN MVKASQLFVG EHDFTAFSNA KSKKKTNTRT IHSITIQDNQ 190 200 210 220 230 240 GFIDIRVCGD GFLYNMVRKM VGTLIEVGLG EKEPEQVLTI LESKDRSQAG FADATGLYLE GISF

References

[1]	"Nucleotide sequence of the gene for an alkaline endoglucanase from an alkalophilic Bacillus and its expression in Escherichia coli and Bacillus subtilis." Sumitomo N., Ozaki K., Kawai S., Ito S. Biosci. Biotechnol. Biochem. 56:872-877(1992) [PubMed: 1368251] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M84963 Genomic DNA. Translation: AAA73188.1.
PIR	JT0612.
3D structure databases
ProteinModelPortal	Q45557.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_00171. TruA. [Tree]
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR001406. PsdUridine_synth_TruA. IPR020097. PsdUridine_synth_TruA_a/b_dom. IPR020095. PsdUridine_synth_TruA_C. IPR020094. PsdUridine_synth_TruA_N. [Graphical view]
Gene3D	G3DSA:3.30.70.580. PseudoU_synth_1. 1 hit. G3DSA:3.30.70.660. PseudoU_synth_1. 1 hit.
PANTHER	PTHR11142. PseudoU_synth_1. 1 hit.
Pfam	PF01416. PseudoU_synth_1. 2 hits. [Graphical view]
PIRSF	PIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00071. HisT_truA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRUA_BACSV

Accession

Primary (citable) accession number: Q45557

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	September 21, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program