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Protein

Modification methylase BglII

Gene

bglIIM

Organism
Bacillus subtilis
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence AGATCT, causes specific methylation on C-5 on both strands, and protects the DNA from cleavage by the BglII endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.113. 658.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase BglII (EC:2.1.1.113)
Short name:
M.BglII
Alternative name(s):
N(4)- cytosine-specific methyltransferase BglII
Gene namesi
Name:bglIIM
OrganismiBacillus subtilis
Taxonomic identifieri1423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 360360Modification methylase BglIIPRO_0000087924Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ45489.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002941. DNA_methylase_N4/N6.
IPR017985. MeTrfase_CN4_CS.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00508. S21N4MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00093. N4_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q45489-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEDQYKQIK LHLGMEDDNE DLPNHIPSSF PKQHLNKIYN GDTMNMLLDI
60 70 80 90 100
PDNSVDLVVT SPPYNINKFK NDRRPLEEYL KWQTEIIEQC HRVLKPSGSI
110 120 130 140 150
FWQVGTYVND SGAHIPLDIR FFPIFESLGM FPRNRIVWVR PHGLHANKKF
160 170 180 190 200
AGRHETILWF TKTPEYKFFL DPIRVPQKYA NKKHYKGDKK GELSGDPLGK
210 220 230 240 250
NPGDVWAFRN VRHNHEEDTI HPTQYPEDMI ERIVLSTTEP NDIVLDPFIG
260 270 280 290 300
MGTTASVAKN LNRYFYGAEI EKEYVDIAYQ ILSGEPDENN NFPNLKTLRQ
310 320 330 340 350
YCEKNGIIDP SQYTFTRQRK GSKPSLDSKA HPEEHHKKEI VERIEFEAEN
360
SVYKKVQNEQ
Length:360
Mass (Da):42,043
Last modified:November 1, 1996 - v1
Checksum:iB1C61DF49E5285D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49842 Genomic DNA. Translation: AAC45061.1.
PIRiJC6322.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49842 Genomic DNA. Translation: AAC45061.1.
PIRiJC6322.

3D structure databases

ProteinModelPortaliQ45489.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi2.1.1.113. 658.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002941. DNA_methylase_N4/N6.
IPR017985. MeTrfase_CN4_CS.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00508. S21N4MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00093. N4_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMTB2_BACIU
AccessioniPrimary (citable) accession number: Q45489
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: November 1, 1996
Last modified: April 13, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.