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Q45488

- T2B2_BACIU

UniProt

Q45488 - T2B2_BACIU

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Protein
Type-2 restriction enzyme BglII
Gene
bglIIR
Organism
Bacillus subtilis
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence AGATCT and cleaves after A-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

Binds 1 magnesium ion per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi84 – 841Magnesium
Metal bindingi94 – 941Magnesium; via carbonyl oxygen

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC
  3. magnesium ion binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Keywords - Ligandi

Magnesium, Metal-binding

Protein family/group databases

REBASEi261. BglII.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme BglII (EC:3.1.21.4)
Short name:
R.BglII
Alternative name(s):
Endonuclease BglII
Type II restriction enzyme BglII
Gene namesi
Name:bglIIR
OrganismiBacillus subtilis
Taxonomic identifieri1423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 223223Type-2 restriction enzyme BglII
PRO_0000077286Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 87
Helixi11 – 144
Helixi17 – 2913
Beta strandi32 – 365
Beta strandi46 – 483
Helixi50 – 6112
Helixi62 – 643
Beta strandi67 – 715
Helixi74 – 796
Beta strandi81 – 877
Beta strandi90 – 945
Helixi100 – 11516
Beta strandi118 – 1236
Beta strandi126 – 1327
Helixi145 – 15713
Beta strandi165 – 1717
Beta strandi178 – 19013
Beta strandi194 – 20512
Beta strandi219 – 2224

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1D2IX-ray1.70A/B1-223[»]
1DFMX-ray1.50A/B1-223[»]
1ES8X-ray2.30A1-223[»]
ProteinModelPortaliQ45488.
SMRiQ45488. Positions 1-223.

Miscellaneous databases

EvolutionaryTraceiQ45488.

Family & Domainsi

Family and domain databases

Gene3Di3.40.91.20. 1 hit.
InterProiIPR011338. BamHI/BglII/BstY.
IPR011335. Restrct_endonuc-II-like.
IPR015278. Restrct_endonuc_II_BglII.
[Graphical view]
PfamiPF09195. Endonuc-BglII. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

Q45488-1 [UniParc]FASTAAdd to Basket

« Hide

MKIDITDYNH ADEILNPQLW KEIEETLLKM PLHVKASDQA SKVGSLIFDP    50
VGTNQYIKDE LVPKHWKNNI PIPKRFDFLG TDIDFGKRDT LVEVQFSNYP 100
FLLNNTVRSE LFHKSNMDID EEGMKVAIII TKGHMFPASN SSLYYEQAQN 150
QLNSLAEYNV FDVPIRLVGL IEDFETDIDI VSTTYADKRY SRTITKRDTV 200
KGKVIDTNTP NTRRRKRGTI VTY 223
Length:223
Mass (Da):25,763
Last modified:November 1, 1996 - v1
Checksum:i4E71D916BABA7A45
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U49842 Genomic DNA. Translation: AAC45060.1.
PIRiJC6323.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U49842 Genomic DNA. Translation: AAC45060.1 .
PIRi JC6323.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1D2I X-ray 1.70 A/B 1-223 [» ]
1DFM X-ray 1.50 A/B 1-223 [» ]
1ES8 X-ray 2.30 A 1-223 [» ]
ProteinModelPortali Q45488.
SMRi Q45488. Positions 1-223.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 261. BglII.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q45488.

Family and domain databases

Gene3Di 3.40.91.20. 1 hit.
InterProi IPR011338. BamHI/BglII/BstY.
IPR011335. Restrct_endonuc-II-like.
IPR015278. Restrct_endonuc_II_BglII.
[Graphical view ]
Pfami PF09195. Endonuc-BglII. 1 hit.
[Graphical view ]
SUPFAMi SSF52980. SSF52980. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of the BglII restriction-modification system reveals a possible evolutionary footprint."
    Anton B.P., Heiter D.F., Benner J.S., Hess E.J., Greenough L., Moran L.S., Slatko B.E., Brooks J.E.
    Gene 187:19-27(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Globigii / RUB562.
  2. "Understanding the immutability of restriction enzymes: crystal structure of BglII and its DNA substrate at 1.5 A resolution."
    Lukacs C.M., Kucera R., Schildkraut I., Aggarwal A.K.
    Nat. Struct. Biol. 7:134-140(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).

Entry informationi

Entry nameiT2B2_BACIU
AccessioniPrimary (citable) accession number: Q45488
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

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