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Q45488 (T2B2_BACIU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme BglII
Short name=R.BglII
EC=3.1.21.4
Alternative name(s):
Endonuclease BglII
Type II restriction enzyme BglII
Gene names
Name:bglIIR
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus ›

Protein attributes

Sequence length223 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes the double-stranded sequence AGATCT and cleaves after A-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactor

Binds 1 magnesium ion per subunit.

Subunit structure

Homodimer.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 223223Type-2 restriction enzyme BglII
PRO_0000077286

Sites

Metal binding841Magnesium
Metal binding941Magnesium; via carbonyl oxygen

Secondary structure

1 ...................................... 223
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q45488 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 4E71D916BABA7A45

FASTA22325,763
        10         20         30         40         50         60 
MKIDITDYNH ADEILNPQLW KEIEETLLKM PLHVKASDQA SKVGSLIFDP VGTNQYIKDE 

        70         80         90        100        110        120 
LVPKHWKNNI PIPKRFDFLG TDIDFGKRDT LVEVQFSNYP FLLNNTVRSE LFHKSNMDID 

       130        140        150        160        170        180 
EEGMKVAIII TKGHMFPASN SSLYYEQAQN QLNSLAEYNV FDVPIRLVGL IEDFETDIDI 

       190        200        210        220 
VSTTYADKRY SRTITKRDTV KGKVIDTNTP NTRRRKRGTI VTY

« Hide

References

[1]"Cloning and characterization of the BglII restriction-modification system reveals a possible evolutionary footprint."
Anton B.P., Heiter D.F., Benner J.S., Hess E.J., Greenough L., Moran L.S., Slatko B.E., Brooks J.E.
Gene 187:19-27(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Globigii / RUB562.
[2]"Understanding the immutability of restriction enzymes: crystal structure of BglII and its DNA substrate at 1.5 A resolution."
Lukacs C.M., Kucera R., Schildkraut I., Aggarwal A.K.
Nat. Struct. Biol. 7:134-140(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U49842 Genomic DNA. Translation: AAC45060.1.
PIRJC6323.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1D2IX-ray1.70A/B1-223[»]
1DFMX-ray1.50A/B1-223[»]
1ES8X-ray2.30A1-223[»]
ProteinModelPortalQ45488.
SMRQ45488. Positions 1-223.
ModBaseSearch...

Protein family/group databases

REBASE261. BglII.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.91.20. 1 hit.
InterProIPR011338. BamHI/BglII/BstY.
IPR011335. Restrct_endonuc-II-like.
IPR015278. Restrct_endonuc_II_BglII.
[Graphical view]
PfamPF09195. Endonuc-BglII. 1 hit.
[Graphical view]
SUPFAMSSF52980. Restrict_endonuc_II-like_core. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ45488.

Entry information

Entry nameT2B2_BACIU
AccessionPrimary (citable) accession number: Q45488
Entry history
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: November 1, 1996
Last modified: April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

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