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Protein

Type-2 restriction enzyme BglII

Gene

bglIIR

Organism
Bacillus subtilis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence AGATCT and cleaves after A-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

Mg2+Note: Binds 1 Mg2+ ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi84Magnesium1
Metal bindingi94Magnesium; via carbonyl oxygen1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Keywords - Ligandi

Magnesium, Metal-binding

Protein family/group databases

REBASEi261. BglII.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme BglII (EC:3.1.21.4)
Short name:
R.BglII
Alternative name(s):
Endonuclease BglII
Type II restriction enzyme BglII
Gene namesi
Name:bglIIR
OrganismiBacillus subtilis
Taxonomic identifieri1423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000772861 – 223Type-2 restriction enzyme BglIIAdd BLAST223

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1223
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 8Combined sources7
Helixi11 – 14Combined sources4
Helixi17 – 29Combined sources13
Beta strandi32 – 36Combined sources5
Beta strandi46 – 48Combined sources3
Helixi50 – 61Combined sources12
Helixi62 – 64Combined sources3
Beta strandi67 – 71Combined sources5
Helixi74 – 79Combined sources6
Beta strandi81 – 87Combined sources7
Beta strandi90 – 94Combined sources5
Helixi100 – 115Combined sources16
Beta strandi118 – 123Combined sources6
Beta strandi126 – 132Combined sources7
Helixi145 – 157Combined sources13
Beta strandi165 – 171Combined sources7
Beta strandi178 – 190Combined sources13
Beta strandi194 – 205Combined sources12
Beta strandi219 – 222Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D2IX-ray1.70A/B1-223[»]
1DFMX-ray1.50A/B1-223[»]
1ES8X-ray2.30A1-223[»]
ProteinModelPortaliQ45488.
SMRiQ45488.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ45488.

Family & Domainsi

Family and domain databases

Gene3Di3.40.91.20. 1 hit.
InterProiIPR011338. BamHI/BglII/BstY.
IPR015278. BglII.
IPR011335. Restrct_endonuc-II-like.
[Graphical view]
PfamiPF09195. Endonuc-BglII. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

Q45488-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIDITDYNH ADEILNPQLW KEIEETLLKM PLHVKASDQA SKVGSLIFDP
60 70 80 90 100
VGTNQYIKDE LVPKHWKNNI PIPKRFDFLG TDIDFGKRDT LVEVQFSNYP
110 120 130 140 150
FLLNNTVRSE LFHKSNMDID EEGMKVAIII TKGHMFPASN SSLYYEQAQN
160 170 180 190 200
QLNSLAEYNV FDVPIRLVGL IEDFETDIDI VSTTYADKRY SRTITKRDTV
210 220
KGKVIDTNTP NTRRRKRGTI VTY
Length:223
Mass (Da):25,763
Last modified:November 1, 1996 - v1
Checksum:i4E71D916BABA7A45
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49842 Genomic DNA. Translation: AAC45060.1.
PIRiJC6323.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49842 Genomic DNA. Translation: AAC45060.1.
PIRiJC6323.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D2IX-ray1.70A/B1-223[»]
1DFMX-ray1.50A/B1-223[»]
1ES8X-ray2.30A1-223[»]
ProteinModelPortaliQ45488.
SMRiQ45488.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi261. BglII.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ45488.

Family and domain databases

Gene3Di3.40.91.20. 1 hit.
InterProiIPR011338. BamHI/BglII/BstY.
IPR015278. BglII.
IPR011335. Restrct_endonuc-II-like.
[Graphical view]
PfamiPF09195. Endonuc-BglII. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiT2B2_BACIU
AccessioniPrimary (citable) accession number: Q45488
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.