Q45479 (LSPA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoprotein signal peptidase
EC=3.4.23.36

Alternative name(s):
Prolipoprotein signal peptidase
Signal peptidase II
Short name=SPase II

Gene names

Name:	lspA
Synonyms:	lsp
Ordered Locus Names:	BSU15450

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	154 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins. HAMAP MF_00161
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161
Subcellular location	Cell membrane; Multi-pass membrane protein HAMAP MF_00161.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 154	154	Lipoprotein signal peptidase HAMAP MF_00161		PRO_0000178769
Regions
	Topological domain	1	1	Cytoplasmic Potential
	Transmembrane	2 – 22	21	Helical; Potential
	Topological domain	23 – 51	29	Extracellular Potential
	Transmembrane	52 – 72	21	Helical; Potential
	Topological domain	73 – 84	12	Cytoplasmic Potential
	Transmembrane	85 – 105	21	Helical; Potential
	Topological domain	106 – 123	18	Extracellular Potential
	Transmembrane	124 – 144	21	Helical; Potential
	Topological domain	145 – 154	10	Cytoplasmic Potential
Sites
	Active site	102	1	Probable
	Active site	129	1	Probable

Sequences

	Sequence		Length	Mass (Da)
	Q45479 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 9229CD9FCA8CBB55	FASTA	154	17,427
	10 20 30 40 50 60 MLYYMIALLI IAADQLTKWL VVKNMELGQS IPIIDQVFYI TSHRNTGAAW GILAGQMWFF 70 80 90 100 110 120 YLITTAVIIG IVYYIQRYTK GQRLLGVALG LMLGGAIGNF IDRAVRQEVV DFIHVIIVNY 130 140 150 NYPIFNIADS SLCVGVMLLF IQMLLDSGKK KKEQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The signal peptidase II (lsp) gene of Bacillus subtilis." Pragai Z., Tjalsma H., Bolhuis A., van Dijl J.M., Venema G., Bron S. Microbiology 143:1327-1333(1997) [PubMed: 9141696] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	Baek-Rak L., Jeong-Hyun K. Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-33.
[4]	"The potential active site of the lipoprotein-specific (type II) signal peptidase of Bacillus subtilis." Tjalsma H., Zanen G., Venema G., Bron S., van Dijl J.M. J. Biol. Chem. 274:28191-28197(1999) [PubMed: 10497172] [Abstract] Cited for: MUTAGENESIS, ACTIVE SITES.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U48870 Genomic DNA. Translation: AAB57766.1. AL009126 Genomic DNA. Translation: CAB13419.1. U08116 Unassigned DNA. No translation available.
PIR	G69653.
RefSeq	NP_389428.1. NC_000964.3.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	A08.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000002354; EBBACP00000002354; EBBACG00000002349.
GeneID	938172.
GenomeReviews	Gene locus BSU15450 in contig AL009126_GR.
KEGG	bsu:BSU15450.
NMPDR	fig|224308.1.peg.1547.
PATRIC	18974897. VBIBacSub10457_1640.
Organism-specific databases
GenoList	BSU15450. [Micado]
Phylogenomic databases
GeneTree	EBGT00050000001848.
HOGENOM	HBG724422.
OMA	QARWITP.
PhylomeDB	Q45479.
ProtClustDB	PRK00376.
Enzyme and pathway databases
BioCyc	BSUB:BSU15450-MONOMER.
Family and domain databases
HAMAP	MF_00161. LspA. [Tree]
InterPro	IPR001872. Peptidase_A8. [Graphical view]
KO	K03101.
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
TIGRFAMs	TIGR00077. LspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_BACSU

Accession

Primary (citable) accession number: Q45479

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program