Reviewed,
UniProtKB/Swiss-Prot Q45479 (LSPA_BACSU)
Last modified
February 9, 2010.
Version 70.
History...
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Lipoprotein signal peptidase EC=3.4.23.36 Alternative name(s): Prolipoprotein signal peptidase Signal peptidase II Short name=SPase II | ||||||
| Gene names |
| ||||||
| Organism | Bacillus subtilis [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 1423 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus |
Protein attributes
| Sequence length | 154 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | This protein specifically catalyzes the removal of signal peptides from prolipoproteins. HAMAP MF_00161 |
| Catalytic activity | Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161 |
| Subcellular location | |
| Sequence similarities | Belongs to the peptidase A8 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell membrane Membrane |
| Domain | Transmembrane |
| Molecular function | Aspartyl protease Hydrolase Protease |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | proteolysis Inferred from electronic annotation. Source: InterPro |
| Cellular component | integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membraneInferred from electronic annotation. Source: HAMAP |
| Molecular function | aspartic-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 154 | 154 | Lipoprotein signal peptidase HAMAP MF_00161 | PRO_0000178769 | |||||
Regions | |||||||||
| Topological domain | 1 | 1 | Cytoplasmic Potential | ||||||
| Transmembrane | 2 – 22 | 21 | Potential | ||||||
| Topological domain | 23 – 51 | 29 | Extracellular Potential | ||||||
| Transmembrane | 52 – 72 | 21 | Potential | ||||||
| Topological domain | 73 – 84 | 12 | Cytoplasmic Potential | ||||||
| Transmembrane | 85 – 105 | 21 | Potential | ||||||
| Topological domain | 106 – 123 | 18 | Extracellular Potential | ||||||
| Transmembrane | 124 – 144 | 21 | Potential | ||||||
| Topological domain | 145 – 154 | 10 | Cytoplasmic Potential | ||||||
Sites | |||||||||
| Active site | 102 | 1 | Probable | ||||||
| Active site | 129 | 1 | Probable | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The signal peptidase II (lsp) gene of Bacillus subtilis." Pragai Z., Tjalsma H., Bolhuis A., van Dijl J.M., Venema G., Bron S. Microbiology 143:1327-1333(1997) [PubMed: 9141696] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168. |
| [2] | "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.  Danchin A.Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168. |
| [3] | Baek-Rak L., Jeong-Hyun K. Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-33. |
| [4] | "The potential active site of the lipoprotein-specific (type II) signal peptidase of Bacillus subtilis." Tjalsma H., Zanen G., Venema G., Bron S., van Dijl J.M. J. Biol. Chem. 274:28191-28197(1999) [PubMed: 10497172] [Abstract] Cited for: MUTAGENESIS, ACTIVE SITES. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U48870 Genomic DNA. Translation: AAB57766.1. AL009126 Genomic DNA. Translation: CAB13419.1. U08116 Unassigned DNA. No translation available. |
| PIR | G69653. |
| RefSeq | NP_389428.1. |
3D structure databases | |
| ModBase | Search... |
Protein family/group databases | |
| MEROPS | A08.001. |
Genome annotation databases | |
| GeneID | 938172. |
| GenomeReviews | Gene locus BSU15450 in contig AL009126_GR. |
| KEGG | bsu:BSU15450. |
| NMPDR | fig|224308.1.peg.1547. |
Organism-specific databases | |
| SubtiList | BG11793. lspA. [Micado] |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | HBG724422. |
| OMA | DQASKRW. |
| PhylomeDB | Q45479. |
Enzyme and pathway databases | |
| BRENDA | 3.4.23.36. 150. |
Family and domain databases | |
| HAMAP | MF_00161. LspA. [Tree] |
| InterPro | IPR001872. Peptidase_A8. [Graphical view] |
| Pfam | PF01252. Peptidase_A8. 1 hit. [Graphical view] |
| PRINTS | PR00781. LIPOSIGPTASE. |
| TIGRFAMs | TIGR00077. lspA. 1 hit. |
| PROSITE | PS00855. SPASE_II. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | LSPA_BACSU | ||||||||
| Accession | Primary (citable) accession number: Q45479 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Bacillus subtilis Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList |
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |
