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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei553Aminoacyl-adenylateUniRule annotation1
Binding sitei597ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU15430-MONOMER
BRENDAi6.1.1.5 658
SABIO-RKQ45477

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BSU15430
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000983511 – 921Isoleucine--tRNA ligaseAdd BLAST921

Proteomic databases

PaxDbiQ45477
PRIDEiQ45477

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

IntActiQ45477, 1 interactor
MINTiQ45477
STRINGi224308.Bsubs1_010100008531

Structurei

3D structure databases

ProteinModelPortaliQ45477
SMRiQ45477
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi594 – 598"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
InParanoidiQ45477
KOiK01870
OMAiHLGTAWN
PhylomeDBiQ45477

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q45477-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFKDTLLMP KTDFPMRGNL PNREPDIQKK WEEEDIYRLV QERTKDRPKF
60 70 80 90 100
VLHDGPPYAN GDIHMGHALN KILKDFIVRY KSMSGYNAPY VPGWDTHGLP
110 120 130 140 150
IETALTKNKK VNRKEMSVAE FRKLCEEYAW KQIEGQREQF KRLGVRGDWE
160 170 180 190 200
NPYVTLKPEY EAQQIRVFGE MAKRGYIYKG LKPVNWSPSS ESALAEAEIE
210 220 230 240 250
YQDKRSASIY VAFGVKDGKG VLENGERIII WTTTPWTIPA NLGISVHPDL
260 270 280 290 300
EYSVIAVGED RFVVASALVE NVASACGFDQ YEVTRTVKGK DLENIIAEHP
310 320 330 340 350
LYGRDSLVML GEHVTTDAGT GCVHTAPGHG EDDFIIGQKY GLDVLCPVDE
360 370 380 390 400
KGVMTSEAPG FEGMFYDDAN KAITQQLDEK GALVKLEFIT HSYPHDWRTK
410 420 430 440 450
KPTIFRATAQ WFASIKDFRS DLLDAIKETK WVPEWGEQRL HNMVRDRGDW
460 470 480 490 500
CISRQRAWGV PIPVFYAENG EPVITDETIE HVSELFRQHG SNIWFEKEAK
510 520 530 540 550
DLLPEGFTHP GSPNGTFTKE QDIMDVWFDS GSSHQAVLEE RDDLVRPADL
560 570 580 590 600
YLEGSDQYRG WFNSSLSTAV AVTGKAPYKG VLSHGFALDG EGRKMSKSIG
610 620 630 640 650
NVVVPAKVMK QLGADILRLW VSSVDYQADV RVSDAILKQV AEVYRKIRNT
660 670 680 690 700
FRFLHGNLFD FDPKTNAVAV EDLREVDQYM LIKLNKLIDK VKKAYDEYEF
710 720 730 740 750
AVVYHSIHNF CTIELSSFYL DFAKDIVYIE HADHPDRRSM QTVFYETLLA
760 770 780 790 800
LVKLSAPILP HTADELWSHL TFVEEQSVQL TDMPETITVP NSEATEEKFD
810 820 830 840 850
RFMALRDDVL KALETARNEK IIGKSLEANL KLYPNKENKE LLASIKENLS
860 870 880 890 900
QLFIVSELTI SEENEAPNDA QSFATGKIAV EKAEGEMCER SRVISKDVGA
910 920
NPKYPTLSLR NAEIVEKYYQ K
Length:921
Mass (Da):104,845
Last modified:June 16, 2009 - v3
Checksum:iF41081EA48E317E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA Translation: CAB13417.2
U60901 Genomic DNA Translation: AAB49280.1
U48870 Genomic DNA Translation: AAB57764.1
PIRiH69643
RefSeqiNP_389426.2, NC_000964.3
WP_003245512.1, NZ_JNCM01000035.1

Genome annotation databases

EnsemblBacteriaiCAB13417; CAB13417; BSU15430
GeneIDi940119
KEGGibsu:BSU15430
PATRICifig|224308.179.peg.1682

Similar proteinsi

Entry informationi

Entry nameiSYI_BACSU
AccessioniPrimary (citable) accession number: Q45477
Secondary accession number(s): O31730, P71022
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 16, 2009
Last modified: March 28, 2018
This is version 135 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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