Q45458 (Q45458_GEOSE) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA polymerase RuleBase RU004460
EC=2.7.7.7 RuleBase RU004460

Gene names

Name:

polA EMBL AAB52611.1

Organism

Geobacillus stearothermophilus (Bacillus stearothermophilus) EMBL AAB52611.1

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	876 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). RuleBase RU004460
Sequence similarities	Belongs to the DNA polymerase type-A family. RuleBase RU004459 Contains 1 5'-3' exonuclease domain. SAAS SAAS002421

Ontologies

Keywords
Biological process	DNA replication RuleBase RU004460
Ligand	DNA-binding RuleBase RU004460
Molecular function	Nucleotidyltransferase RuleBase RU004460 Transferase
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro 5'-3' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q45458 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: B981BCE95F1651A7	FASTA	876	99,009
	10 20 30 40 50 60 MKKKLVLIDG NSVAYRAFFA LPLLHNDKGI HTNAVYGFTM MLNKILAEEQ PTHLLVAFDA 70 80 90 100 110 120 GKTTFRHETF QEYKGGRQQT PPELSEQFPL LRELLKAYRI PAYELDHYEA DDIIGTLAAR 130 140 150 160 170 180 AEQEGFEVKI ISGDRDLTQL ASRHVTVDIT KKGITDIEPY TPETVREKYG LTPEQIVDLK 190 200 210 220 230 240 GLMGDKSDNI PGVPGIGEKT AVKLLKQFGT VENVLASIDE VKGEKLKENL RQHRDLALLS 250 260 270 280 290 300 KQLASICRDA PVELSLDDIV YEGQDREKVI ALFKELGFQS FLEKMAAPAA EGEKPLEEME 310 320 330 340 350 360 FAIVDVITEE MLADKAALVV EVMEENYHDA PIVGIALVNE HGRFFMRPET ALADSQFLAW 370 380 390 400 410 420 LADETKKKSM FDAKRAVVAL KWKGIELRGV AFDLLLAAYL LNPAQDAGDI AAVAKMKQYE 430 440 450 460 470 480 AVRSDEAVYG KGVKRSLPDE QTLAEHLVRK AAAIWALEQP FMDDLRNNEQ DQLLTKLEQP 490 500 510 520 530 540 LAAILAEMEF TGVNVDTKRL EQMGSELAEQ LRAIEQRIYE LAGQEFNINS PKQLGVILFE 550 560 570 580 590 600 KLQLPVLKKT KTGYSTSADV LEKLAPHHEI VENILHYRQL GKLQSTYIEG LLKVVRPDTG 610 620 630 640 650 660 KVHTMFNQAL TQTGRLSSAE PNLQNIPIRL EEGRKIRQAF VPSEPDWLIF AADYSQIELR 670 680 690 700 710 720 VLAHIADDDN LIEAFQRDLD IHTKTAMDIF HVSEEEVTAN MRRQAKAVNF GIVYGISDYG 730 740 750 760 770 780 LAQNLNITRK EAAEFIERYF ASFPGVKQYM ENIVQEAKQK GYVTTLLHRR RYLPDITSRN 790 800 810 820 830 840 FNVRSFAERT AMNTPIQGSA ADIIKKAMID LAARLKEEQL QARLLLQVHD ELILEAPKEE 850 860 870 IERLCELVPE VMEQAVTLRV PLKVDYHYGP TWYDAK

References

[1]	"Thermostable Bst DNA polymerase I lacks a 3'-->5' proofreading exonuclease activity." Aliotta J.M., Pelletier J.J., Ware J.L., Moran L.S., Benner J.S., Kong H. Genet. Anal. 12:185-195(1996) Cited for: NUCLEOTIDE SEQUENCE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U33536 Genomic DNA. Translation: AAB52611.1.
3D structure databases
HSSP	HSSP built from PDB template 1L3T based on UniProtKB P52026.
ProteinModelPortal	Q45458.
SMR	Q45458. Positions 299-876.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002562. 3'-5'_exonuclease_dom. IPR020046. 5-3_exonucl_a-hlix_arch_N. IPR020045. 5-3_exonuclease_C. IPR002421. 5-3_exonuclease_N. IPR019760. DNA-dir_DNA_pol_A_CS. IPR001098. DNA-dir_DNA_pol_A_palm_dom. IPR018320. DNA_polymerase_1. IPR002298. DNA_polymerase_A. IPR008918. HhH2. IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif. IPR012337. RNaseH-like_dom. [Graphical view]
Pfam	PF01367. 5_3_exonuc. 1 hit. PF02739. 5_3_exonuc_N. 1 hit. PF00476. DNA_pol_A. 1 hit. [Graphical view]
PRINTS	PR00868. DNAPOLI.
SMART	SM00474. 35EXOc. 1 hit. SM00475. 53EXOc. 1 hit. SM00278. HhH1. 1 hit. SM00279. HhH2. 1 hit. SM00482. POLAc. 1 hit. [Graphical view]
SUPFAM	SSF47807. 5_3_exo_C. 1 hit. SSF53098. RNaseH_fold. 1 hit.
TIGRFAMs	TIGR00593. Pola. 1 hit.
PROSITE	PS00447. DNA_POLYMERASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q45458_GEOSE

Accession

Primary (citable) accession number: Q45458

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1996
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)