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Protein

DNA polymerase

Gene

polA

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi653 – 6531MagnesiumCombined sources
Metal bindingi654 – 6541Magnesium; via carbonyl oxygenCombined sources
Metal bindingi830 – 8301MagnesiumCombined sources

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, NucleaseSAAS annotation, NucleotidyltransferaseUniRule annotation, Transferase

Keywords - Biological processi

DNA replicationUniRule annotation

Keywords - Ligandi

DNA-bindingUniRule annotation, MagnesiumCombined sources, Metal-bindingCombined sources

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymeraseUniRule annotation (EC:2.7.7.7UniRule annotation)
Gene namesi
Name:polAImported
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)Imported
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HHQX-ray1.80A308-876[»]
2HHSX-ray1.80A308-876[»]
2HHTX-ray2.05A308-876[»]
2HVHX-ray2.49A/D308-876[»]
2HW3X-ray1.98A308-876[»]
ProteinModelPortaliQ45458.
SMRiQ45458. Positions 299-876.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 26226053EXOcInterPro annotationAdd
BLAST
Domaini300 – 4661673'-5' exonucleaseInterPro annotationAdd
BLAST
Domaini633 – 840208POLAcInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the DNA polymerase type-A family.UniRule annotation

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.40.50.1010. 1 hit.
InterProiIPR002562. 3'-5'_exonuclease_dom.
IPR020046. 5-3_exonucl_a-hlix_arch_N.
IPR020045. 5-3_exonuclease_C.
IPR002421. 5-3_exonuclease_N.
IPR019760. DNA-dir_DNA_pol_A_CS.
IPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR018320. DNA_polymerase_1.
IPR002298. DNA_polymerase_A.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF01367. 5_3_exonuc. 1 hit.
PF02739. 5_3_exonuc_N. 1 hit.
PF00476. DNA_pol_A. 1 hit.
[Graphical view]
PRINTSiPR00868. DNAPOLI.
SMARTiSM00474. 35EXOc. 1 hit.
SM00475. 53EXOc. 1 hit.
SM00279. HhH2. 1 hit.
SM00482. POLAc. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF53098. SSF53098. 1 hit.
SSF88723. SSF88723. 1 hit.
TIGRFAMsiTIGR00593. pola. 1 hit.
PROSITEiPS00447. DNA_POLYMERASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q45458-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKKLVLIDG NSVAYRAFFA LPLLHNDKGI HTNAVYGFTM MLNKILAEEQ
60 70 80 90 100
PTHLLVAFDA GKTTFRHETF QEYKGGRQQT PPELSEQFPL LRELLKAYRI
110 120 130 140 150
PAYELDHYEA DDIIGTLAAR AEQEGFEVKI ISGDRDLTQL ASRHVTVDIT
160 170 180 190 200
KKGITDIEPY TPETVREKYG LTPEQIVDLK GLMGDKSDNI PGVPGIGEKT
210 220 230 240 250
AVKLLKQFGT VENVLASIDE VKGEKLKENL RQHRDLALLS KQLASICRDA
260 270 280 290 300
PVELSLDDIV YEGQDREKVI ALFKELGFQS FLEKMAAPAA EGEKPLEEME
310 320 330 340 350
FAIVDVITEE MLADKAALVV EVMEENYHDA PIVGIALVNE HGRFFMRPET
360 370 380 390 400
ALADSQFLAW LADETKKKSM FDAKRAVVAL KWKGIELRGV AFDLLLAAYL
410 420 430 440 450
LNPAQDAGDI AAVAKMKQYE AVRSDEAVYG KGVKRSLPDE QTLAEHLVRK
460 470 480 490 500
AAAIWALEQP FMDDLRNNEQ DQLLTKLEQP LAAILAEMEF TGVNVDTKRL
510 520 530 540 550
EQMGSELAEQ LRAIEQRIYE LAGQEFNINS PKQLGVILFE KLQLPVLKKT
560 570 580 590 600
KTGYSTSADV LEKLAPHHEI VENILHYRQL GKLQSTYIEG LLKVVRPDTG
610 620 630 640 650
KVHTMFNQAL TQTGRLSSAE PNLQNIPIRL EEGRKIRQAF VPSEPDWLIF
660 670 680 690 700
AADYSQIELR VLAHIADDDN LIEAFQRDLD IHTKTAMDIF HVSEEEVTAN
710 720 730 740 750
MRRQAKAVNF GIVYGISDYG LAQNLNITRK EAAEFIERYF ASFPGVKQYM
760 770 780 790 800
ENIVQEAKQK GYVTTLLHRR RYLPDITSRN FNVRSFAERT AMNTPIQGSA
810 820 830 840 850
ADIIKKAMID LAARLKEEQL QARLLLQVHD ELILEAPKEE IERLCELVPE
860 870
VMEQAVTLRV PLKVDYHYGP TWYDAK
Length:876
Mass (Da):99,009
Last modified:November 1, 1996 - v1
Checksum:iB981BCE95F1651A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33536 Genomic DNA. Translation: AAB52611.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33536 Genomic DNA. Translation: AAB52611.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HHQX-ray1.80A308-876[»]
2HHSX-ray1.80A308-876[»]
2HHTX-ray2.05A308-876[»]
2HVHX-ray2.49A/D308-876[»]
2HW3X-ray1.98A308-876[»]
ProteinModelPortaliQ45458.
SMRiQ45458. Positions 299-876.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.40.50.1010. 1 hit.
InterProiIPR002562. 3'-5'_exonuclease_dom.
IPR020046. 5-3_exonucl_a-hlix_arch_N.
IPR020045. 5-3_exonuclease_C.
IPR002421. 5-3_exonuclease_N.
IPR019760. DNA-dir_DNA_pol_A_CS.
IPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR018320. DNA_polymerase_1.
IPR002298. DNA_polymerase_A.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF01367. 5_3_exonuc. 1 hit.
PF02739. 5_3_exonuc_N. 1 hit.
PF00476. DNA_pol_A. 1 hit.
[Graphical view]
PRINTSiPR00868. DNAPOLI.
SMARTiSM00474. 35EXOc. 1 hit.
SM00475. 53EXOc. 1 hit.
SM00279. HhH2. 1 hit.
SM00482. POLAc. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF53098. SSF53098. 1 hit.
SSF88723. SSF88723. 1 hit.
TIGRFAMsiTIGR00593. pola. 1 hit.
PROSITEiPS00447. DNA_POLYMERASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Thermostable Bst DNA polymerase I lacks a 3'-->5' proofreading exonuclease activity."
    Aliotta J.M., Pelletier J.J., Ware J.L., Moran L.S., Benner J.S., Kong H.
    Genet. Anal. 12:185-195(1996)
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "The structural basis for the mutagenicity of O(6)-methyl-guanine lesions."
    Warren J.J., Forsberg L.J., Beese L.S.
    Proc. Natl. Acad. Sci. U.S.A. 103:19701-19706(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 308-876 IN COMPLEX WITH MAGNESIUM.

Entry informationi

Entry nameiQ45458_GEOSE
AccessioniPrimary (citable) accession number: Q45458
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.