Q45423 (TFDA_BURSR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-ketoglutarate-dependent 2,4-dichlorophenoxyacetate dioxygenase
Short name=2,4-D dioxygenase
EC=1.14.11.-

Gene names

Name:

tfdA

Organism

Burkholderia sp. (strain RASC)

Taxonomic identifier

69003 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in degradation of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). Is also able to degrade 2-methyl-4-chlorophenoxyacetic acid and 3-chlorobenzoic acid.
Catalytic activity	2,4-dichlorophenoxyacetate + 2-oxoglutarate + O2 = 2,4-dichlorophenol + glyoxalate + succinate + CO2.
Cofactor	Binds 1 Fe2+ ion per subunit By similarity.
Enzyme regulation	Activated by ascorbate Potential.
Pathway	Xenobiotic degradation; (2,4-dichlorophenoxy)acetate degradation.
Sequence similarities	Belongs to the TfdA dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological_process	2,4-dichlorophenoxyacetic acid catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	Alpha-ketoglutarate-dependent 2,4-dichlorophenoxyacetate dioxygenase		PRO_0000194018
Sites
	Metal binding	114	1	Iron; catalytic By similarity
	Metal binding	116	1	Iron; catalytic By similarity
	Metal binding	263	1	Iron; catalytic By similarity
	Binding site	141	1	2-oxoglutarate By similarity
	Binding site	248	1	2-oxoglutarate By similarity
	Binding site	274	1	2-oxoglutarate By similarity
	Binding site	278	1	2-oxoglutarate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q45423 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 17BF90A067C511FD	FASTA	297	33,638
	10 20 30 40 50 60 MSINSEYLHP LFVGQVDNLA LQGALSPAEV RDVENEMDQK AVLVFRGQPL DQDQQIAFAR 70 80 90 100 110 120 NFGQLEGGFI KVNQRPSRFK YAELADISNV SVDGKVAEAD AREVVGNFAN QLWHSDSSFQ 130 140 150 160 170 180 QPAARYSMLS AIVLPPSGGD TEFCDMRAAY DDLPEDFKKE LQGLRAEHYA LHSRFILGDT 190 200 210 220 230 240 EYSESQRNAM PPVSWPLIRT HAGSGRKFLF IGAHASHIEG RPVAEGRMLL AELLEHATQP 250 260 270 280 290 KFVYRHSWKV GDLVMWDNRC VLHRGRRYDV TARRELRRAT TLGRRCRLSP RGQSWVQ

References

[1]

"Characterization of a chromosomally encoded 2,4-dichlorophenoxyacetic acid/alpha-ketoglutarate dioxygenase from Burkholderia sp. strain RASC." Suwa Y., Wright A.D., Fukimori F., Nummy K.A., Hausinger R.P., Holben W.E., Forney L.J. Appl. Environ. Microbiol. 62:2464-2469(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U25717 Genomic DNA. Translation: AAB17363.1.
3D structure databases
ProteinModelPortal	Q45423.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00685.
Family and domain databases
InterPro	IPR003819. Taurine_dOase. [Graphical view]
Pfam	PF02668. TauD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TFDA_BURSR

Accession

Primary (citable) accession number: Q45423

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program