Reviewed, UniProtKB/Swiss-Prot Q45421 (MTLD_BACST)

Names and origin

Protein names

Recommended name:
    Mannitol-1-phosphate 5-dehydrogenase
    EC=1.1.1.17

Gene names

Name:

mtlD

Organism

Bacillus stearothermophilus (Geobacillus stearothermophilus)

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-mannitol 1-phosphate + NAD(+) = D-fructose 6-phosphate + NADH. HAMAP MF_00196
Sequence similarities	Belongs to the mannitol dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro mannitol-1-phosphate 5-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	Mannitol-1-phosphate 5-dehydrogenase HAMAP MF_00196		PRO_0000170697
Regions
	Nucleotide binding	3 – 14	12	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q45421-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 6A60B887A8C20052	FASTA	382	43,168
	10 20 30 40 50 60 MLAVHFGAGN IGRGFIGSLL SQSGYEVVFV DINDELVRLL KEKQEYRVII ADENRQELLI 70 80 90 100 110 120 RNVSAVNSQT EREKVIDYIT KAHLITTAVG PHILPAIATI LAEGLQKRIT INKTPLHIIA 130 140 150 160 170 180 CENMIGGSDV LKSHVFEKIS EADKPLFEKY YGFLNCAVDR IVPNQKHDDP LSVVVEPFFE 190 200 210 220 230 240 WVIEKRNIIG AIPPIQGAHF VDDLKPYIER KLFTVNTGHA IASYLGYYKK LQTIQEAMCD 250 260 270 280 290 300 QEIRSDVEKA LHESGAVLVK KYGWNENEHQ SYIQKIIQRF INPSISDEVV RVARSPIRKL 310 320 330 340 350 360 GANDRLIGPA TQYYDLFGQV PHGLVKGIAA LLLFDYENDE EAVALQKTIQ ETGVEGALYQ 370 380 YSQLEKDHPL VIAIKDQWQH LK

References

[1]

"Cloning, expression, and isolation of the mannitol transport protein from the thermophilic bacterium Bacillus stearothermophilus." Henstra S.A., Tolner B., ten Hoeve Duurkens R.H., Konings W.N., Robillard G.T. J. Bacteriol. 178:5586-5591(1996) [PubMed: 8824601] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 29609 / DSM 2027 / NCA 1503 / NCIB 8924.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U18943 Genomic DNA. Translation: AAC44466.1.
3D structure databases
ModBase	Search...
Family and domain databases
HAMAP	MF_00196. [Tree]
InterPro	IPR002110. ANK. IPR013328. DHase_multihelical. IPR013118. Mannitol_DHase_C. IPR000669. Mannitol_DHase_core. IPR013131. Mannitol_DHase_N. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
Pfam	PF01232. Mannitol_dh. 1 hit. PF08125. Mannitol_dh_C. 1 hit. [Graphical view]
PRINTS	PR01415. ANKYRIN. PR00084. MTLDHDRGNASE.
PROSITE	PS00974. MANNITOL_DHGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTLD_BACST

Accession

Primary (citable) accession number: Q45421

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 25, 2008
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)