ID   PTEB_BACST              Reviewed;         100 AA.
AC   Q45399;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 53.
DE   RecName: Full=Cellobiose-specific phosphotransferase enzyme IIB component;
DE            EC=2.7.1.69;
DE   AltName: Full=PTS system cellobiose-specific EIIB component;
DE   AltName: Full=EIIB-Cel;
GN   Name=celA;
OS   Bacillus stearothermophilus (Geobacillus stearothermophilus).
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Geobacillus.
OX   NCBI_TaxID=1422;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=XL-65-6;
RX   MEDLINE=94012514; PubMed=8407820;
RA   Lai X., Ingram L.O.;
RT   "Cloning and sequencing of a cellobiose phosphotransferase system
RT   operon from Bacillus stearothermophilus XL-65-6 and functional
RT   expression in Escherichia coli.";
RL   J. Bacteriol. 175:6441-6450(1993).
CC   -!- FUNCTION: The phosphoenolpyruvate-dependent sugar
CC       phosphotransferase system (sugar PTS), a major carbohydrate active
CC       -transport system, catalyzes the phosphorylation of incoming sugar
CC       substrates concomitantly with their translocation across the cell
CC       membrane. This system is involved in cellobiose transport.
CC   -!- CATALYTIC ACTIVITY: Protein EIIB N(pi)-phospho-L-
CC       histidine/cysteine + sugar = protein EIIB + sugar phosphate.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Potential).
CC   -!- DOMAIN: The EIIB domain is phosphorylated by phospho-EIIA on a
CC       cysteinyl or histidyl residue, depending on the transported sugar.
CC       Then, it transfers the phosphoryl group to the sugar substrate
CC       concomitantly with the sugar uptake processed by the EIIC domain.
CC   -!- SIMILARITY: Contains 1 PTS EIIB type-3 domain.
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DR   EMBL; U07818; AAA17389.1; -; Unassigned_DNA.
DR   PIR; B49898; B49898.
DR   HSSP; P17409; 1IIB.
DR   BRENDA; 2.7.1.69; 266715.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosph...; IEA:EC.
DR   GO; GO:0005529; F:sugar binding; IEA:InterPro.
DR   GO; GO:0005351; F:sugar:hydrogen symporter activity; IEA:UniProtKB-KW.
DR   GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phospho...; IEA:UniProtKB-KW.
DR   InterPro; IPR014350; PTrfase_system_EIIB_3_subgr.
DR   InterPro; IPR013012; PTS_EIIB_3.
DR   InterPro; IPR003501; PTS_IIB_lac.
DR   Pfam; PF02302; PTS_IIB; 1.
DR   TIGRFAMs; TIGR00853; pts-lac; 1.
DR   PROSITE; PS51100; PTS_EIIB_TYPE_3; 1.
PE   4: Predicted;
KW   Cytoplasm; Kinase; Phosphotransferase system; Sugar transport;
KW   Transferase; Transport.
FT   CHAIN         1    100       Cellobiose-specific phosphotransferase
FT                                enzyme IIB component.
FT                                /FTId=PRO_0000186486.
FT   DOMAIN        1    100       PTS EIIB type-3.
FT   ACT_SITE      7      7       Phosphocysteine intermediate (By
FT                                similarity).
SQ   SEQUENCE   100 AA;  10737 MW;  7CCD2F32C72F1747 CRC64;
     MNILLICAAG MSTSLLVTKM KEAAKQKGIE ANIWAVSADE AKSHLDQADV VLIGPQIRYK
     LAAFKKEGEA RGIPVDVINP ADYGRVNGAG VLDFALRLKK
//