Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

BrkA autotransporter

Gene

brkA

Organism
Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits the classical pathway of complement activation and prevents accumulation of deposited C4.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Virulence

Enzyme and pathway databases

BioCyciBPER257313:BP3494-MONOMER.

Protein family/group databases

TCDBi1.B.12.2.3. the autotransporter-1 (at-1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
BrkA autotransporter
Cleaved into the following 2 chains:
Gene namesi
Name:brkA
Ordered Locus Names:BP3494
OrganismiBordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)
Taxonomic identifieri257313 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella
Proteomesi
  • UP000002676 Componenti: Chromosome

Subcellular locationi

BrkA translocator :

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane, Periplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 42421 PublicationAdd
BLAST
Chaini43 – 1010968BrkA autotransporterPRO_0000399092Add
BLAST
Chaini43 – 731689Serum resistance protein BrkAPRO_0000399093Add
BLAST
Chaini732 – 1010279BrkA translocatorPRO_0000399094Add
BLAST

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei731 – 7322Cleavage

Interactioni

Protein-protein interaction databases

STRINGi257313.BP3494.

Structurei

Secondary structure

1
1010
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi747 – 76014Combined sources
Beta strandi768 – 78316Combined sources
Beta strandi788 – 80417Combined sources
Beta strandi806 – 8083Combined sources
Beta strandi812 – 84635Combined sources
Beta strandi850 – 8523Combined sources
Beta strandi856 – 87217Combined sources
Helixi877 – 8793Combined sources
Beta strandi880 – 89314Combined sources
Beta strandi896 – 8994Combined sources
Beta strandi905 – 9084Combined sources
Beta strandi912 – 92413Combined sources
Beta strandi929 – 9313Combined sources
Beta strandi933 – 94715Combined sources
Beta strandi967 – 97812Combined sources
Turni979 – 9813Combined sources
Beta strandi982 – 99514Combined sources
Beta strandi997 – 100812Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3QQ2X-ray3.00A/B/C727-1010[»]
ProteinModelPortaliQ45340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini742 – 1010269AutotransporterPROSITE-ProRule annotationAdd
BLAST

Domaini

The signal peptide, cleaved at the inner membrane, guides the autotransporter protein to the periplasmic space. Then, insertion of the C-terminal translocator domain in the outer membrane forms a hydrophilic pore for the translocation of the passenger domain to the bacterial cell surface, with subsequent cleavage. Finally, the mature protein remains tightly associated with the bacterium.
A 31- to 39-amino-acid region found immediately upstream of the translocator domain is essential for surface expression.

Sequence similaritiesi

Contains 1 autotransporter (TC 1.B.12) domain. [View classification]PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4108P8G. Bacteria.
COG3468. LUCA.
KOiK12683.
OMAiNFLNIGY.

Family and domain databases

Gene3Di2.160.20.20. 1 hit.
2.40.128.130. 1 hit.
InterProiIPR005546. Autotransporte_beta.
IPR006315. OM_autotransptr_brl.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
IPR004899. Pertactin_central.
IPR003991. Pertactin_virulence_factor.
[Graphical view]
PfamiPF03797. Autotransporter. 1 hit.
PF03212. Pertactin. 1 hit.
[Graphical view]
PRINTSiPR01484. PRTACTNFAMLY.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF51126. SSF51126. 3 hits.
TIGRFAMsiTIGR01414. autotrans_barl. 1 hit.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q45340-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYLDRFRQCP SSLQIPRSAW RLHALAAALA LAGMARLAPA AAQAPQPPVA
60 70 80 90 100
GAPHAQDAGQ EGEFDHRDNT LIAVFDDGVG INLDDDPDEL GETAPPTLKD
110 120 130 140 150
IHISVEHKNP MSKPAIGVRV SGAGRALTLA GSTIDATEGG IPAVVRRGGT
160 170 180 190 200
LELDGVTVAG GEGMEPMTVS DAGSRLSVRG GVLGGEAPGV GLVRAAQGGQ
210 220 230 240 250
ASIIDATLQS ILGPALIADG GSISVAGGSI DMDMGPGFPP PPPPLPGAPL
260 270 280 290 300
AAHPPLDRVA AVHAGQDGKV TLREVALRAH GPQATGVYAY MPGSEITLQG
310 320 330 340 350
GTVSVQGDDG AGVVAGAGLL DALPPGGTVR LDGTTVSTDG ANTDAVLVRG
360 370 380 390 400
DAARAEVVNT VLRTAKSLAA GVSAQHGGRV TLRQTRIETA GAGAEGISVL
410 420 430 440 450
GFEPQSGSGP ASVDMQGGSI TTTGNRAAGI ALTHGSARLE GVAVRAEGSG
460 470 480 490 500
SSAAQLANGT LVVSAGSLAS AQSGAISVTD TPLKLMPGAL ASSTVSVRLT
510 520 530 540 550
DGATAQGGNG VFLQQHSTIP VAVALESGAL ARGDIVADGN KPLDAGISLS
560 570 580 590 600
VASGAAWHGA TQVLQSATLG KGGTWVVNAD SRVQDMSMRG GRVEFQAPAP
610 620 630 640 650
EASYKTLTLQ TLDGNGVFVL NTNVAAGQND QLRVTGRADG QHRVLVRNAG
660 670 680 690 700
GEADSRGARL GLVHTQGQGN ATFRLANVGK AVDLGTWRYS LAEDPKTHVW
710 720 730 740 750
SLQRAGQALS GAANAAVNAA DLSSIALAES NALDKRLGEL RLRADAGGPW
760 770 780 790 800
ARTFSERQQI SNRHARAYDQ TVSGLEIGLD RGWSASGGRW YAGGLLGYTY
810 820 830 840 850
ADRTYPGDGG GKVKGLHVGG YAAYVGDGGY YLDTVLRLGR YDQQYNIAGT
860 870 880 890 900
DGGRVTADYR TSGAAWSLEG GRRFELPNDW FAEPQAEVML WRTSGKRYRA
910 920 930 940 950
SNGLRVKVDA NTATLGRLGL RFGRRIALAG GNIVQPYARL GWTQEFKSTG
960 970 980 990 1000
DVRTNGIGHA GAGRHGRVEL GAGVDAALGK GHNLYASYEY AAGDRINIPW
1010
SFHAGYRYSF
Length:1,010
Mass (Da):103,377
Last modified:November 1, 1996 - v1
Checksum:i608A006EC3087B52
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti47 – 471P → A AA sequence (PubMed:12511495).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12276 Genomic DNA. Translation: AAA51646.1.
BX640421 Genomic DNA. Translation: CAE43755.1.
PIRiI40329.
RefSeqiNP_882013.1. NC_002929.2.
WP_010931506.1. NC_002929.2.

Genome annotation databases

EnsemblBacteriaiCAE43755; CAE43755; BP3494.
GeneIDi2664892.
KEGGibpe:BP3494.
PATRICi21160628. VBIBorPer7866_3783.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12276 Genomic DNA. Translation: AAA51646.1.
BX640421 Genomic DNA. Translation: CAE43755.1.
PIRiI40329.
RefSeqiNP_882013.1. NC_002929.2.
WP_010931506.1. NC_002929.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3QQ2X-ray3.00A/B/C727-1010[»]
ProteinModelPortaliQ45340.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi257313.BP3494.

Protein family/group databases

TCDBi1.B.12.2.3. the autotransporter-1 (at-1) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE43755; CAE43755; BP3494.
GeneIDi2664892.
KEGGibpe:BP3494.
PATRICi21160628. VBIBorPer7866_3783.

Phylogenomic databases

eggNOGiENOG4108P8G. Bacteria.
COG3468. LUCA.
KOiK12683.
OMAiNFLNIGY.

Enzyme and pathway databases

BioCyciBPER257313:BP3494-MONOMER.

Family and domain databases

Gene3Di2.160.20.20. 1 hit.
2.40.128.130. 1 hit.
InterProiIPR005546. Autotransporte_beta.
IPR006315. OM_autotransptr_brl.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
IPR004899. Pertactin_central.
IPR003991. Pertactin_virulence_factor.
[Graphical view]
PfamiPF03797. Autotransporter. 1 hit.
PF03212. Pertactin. 1 hit.
[Graphical view]
PRINTSiPR01484. PRTACTNFAMLY.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF51126. SSF51126. 3 hits.
TIGRFAMsiTIGR01414. autotrans_barl. 1 hit.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBRKA_BORPE
AccessioniPrimary (citable) accession number: Q45340
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.