Q45321 (OM25_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
25 kDa outer-membrane immunogenic protein

Gene names

Name:	omp25
Ordered Locus Names:	BMEI1249

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella melitensis › Brucella melitensis bv. 1

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Subcellular location	Cell outer membrane.
Sequence similarities	Belongs to the Omp25/RopB family.

Ontologies

Keywords
Cellular component	Cell membrane Cell outer membrane Membrane
Domain	Signal Transmembrane Transmembrane beta strand
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	cell outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 213	190	25 kDa outer-membrane immunogenic protein		PRO_0000021880

Sequences

	Sequence		Length	Mass (Da)
	Q45321 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 59003CF46F6D0ED3	FASTA	213	23,185
	10 20 30 40 50 60 MRTLKSLVIV SAALLPFSAT AFAADAIQEQ PPVPAPVEVA PQYSWAGGYT GLYLGYGWNK 70 80 90 100 110 120 AKTSTVGSIK PDDWKAGAFA GWNFQQDQIV YGVEGDAGYS WAKKSKDGLE VKQGFEGSLR 130 140 150 160 170 180 ARVGYDLNPV MPYLTAGIAG SQIKLNNGLD DESKFRVGWT AGAGLEAKLT DNILGRVEYR 190 200 210 YTQYGNKNYD LAGTTVRNKL DTQDFRVGIG YKF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and expression of the gene encoding the major 25-kilodalton outer membrane protein of Brucella ovis: evidence for antigenic shift, compared with other Brucella species, due to a deletion in the gene." Cloeckaert A., Verger J.M., Grayon M., Zygmunt M.S., Grepinet O. Infect. Immun. 64:2047-2055(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.
[2]	"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U33003 Genomic DNA. Translation: AAB06701.1. AE008917 Genomic DNA. Translation: AAL52430.1.
PIR	AC3408.
RefSeq	NP_540166.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q45321.
ModBase	Search...
Protein-protein interaction databases
STRING	224914.BMEI1249.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL52430; AAL52430; BMEI1249.
GeneID	1196960.
KEGG	bme:BMEI1249.
PATRIC	17797483. VBIBruMel146950_0560.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000135658.
KO	K16079.
OMA	TPEILLY.
ProtClustDB	CLSK898362.
Family and domain databases
Gene3D	2.40.160.20. 1 hit.
InterPro	IPR000758. Enterovir_OMP. IPR011250. OMP/PagP_b-brl. [Graphical view]
SUPFAM	SSF56925. SSF56925. 1 hit.
ProtoNet	Search...

Entry information

Entry name

OM25_BRUME

Accession

Primary (citable) accession number: Q45321

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program