ID   PTG3B_CORGL             Reviewed;         674 AA.
AC   Q45298;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 67.
DE   RecName: Full=PTS system glucose-specific EIIBCA component;
DE   AltName: Full=EIIBCA-Glc;
DE   AltName: Full=EII-Glc/EIII-Glc;
DE   Includes:
DE     RecName: Full=Glucose-specific phosphotransferase enzyme IIB component;
DE              EC=2.7.1.69;
DE     AltName: Full=PTS system glucose-specific EIIB component;
DE   Includes:
DE     RecName: Full=Glucose permease IIC component;
DE     AltName: Full=PTS system glucose-specific EIIC component;
DE   Includes:
DE     RecName: Full=Glucose-specific phosphotransferase enzyme IIA component;
DE              EC=2.7.1.-;
DE     AltName: Full=PTS system glucose-specific EIIA component;
GN   Name=ptsG;
OS   Corynebacterium glutamicum (Brevibacterium flavum).
OG   Plasmid pBSBG2.
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Corynebacteriaceae; Corynebacterium.
OX   NCBI_TaxID=1718;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 13869 / DSMZ 1412 / NCIMB 9567;
RA   Yoon K.-H.;
RT   "Cloning and nucleotide sequence of enzyme II of Brevibacterium
RT   lactofermentum phosphotransferase system.";
RL   Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The phosphoenolpyruvate-dependent sugar
CC       phosphotransferase system (sugar PTS), a major carbohydrate active
CC       -transport system, catalyzes the phosphorylation of incoming sugar
CC       substrates concomitantly with their translocation across the cell
CC       membrane. This system is involved in glucose transport (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: Protein EIIA N(pi)-phospho-L-histidine +
CC       protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-
CC       histidine/cysteine.
CC   -!- CATALYTIC ACTIVITY: Protein EIIB N(pi)-phospho-L-
CC       histidine/cysteine + sugar = protein EIIB + sugar phosphate.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein
CC       (Potential).
CC   -!- DOMAIN: The EIIB domain is phosphorylated by phospho-EIIA on a
CC       cysteinyl or histidyl residue, depending on the transported sugar.
CC       Then, it transfers the phosphoryl group to the sugar substrate
CC       concomitantly with the sugar uptake processed by the EIIC domain.
CC   -!- DOMAIN: The EIIC domain forms the PTS system translocation channel
CC       and contains the specific substrate-binding site.
CC   -!- DOMAIN: The EIIA domain is phosphorylated by phospho-HPr on a
CC       histidyl residue. Then, it transfers the phosphoryl group to the
CC       EIIB domain.
CC   -!- SIMILARITY: Contains 1 PTS EIIA type-1 domain.
CC   -!- SIMILARITY: Contains 1 PTS EIIB type-1 domain.
CC   -!- SIMILARITY: Contains 1 PTS EIIC type-1 domain.
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DR   EMBL; L18875; AAA22992.1; -; Genomic_DNA.
DR   HSSP; P08837; 1GLC.
DR   BRENDA; 2.7.1.69; 812.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosph...; IEA:EC.
DR   GO; GO:0005351; F:sugar:hydrogen symporter activity; IEA:UniProtKB-KW.
DR   GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phospho...; IEA:UniProtKB-KW.
DR   InterPro; IPR018113; PTrfase_EIIB/Cys_phospho_site.
DR   InterPro; IPR001127; PTS_EIIA_1_perm.
DR   InterPro; IPR001996; PTS_EIIB.
DR   InterPro; IPR003352; PTS_EIIC.
DR   InterPro; IPR013013; PTS_EIIC_1.
DR   Gene3D; G3DSA:3.30.1360.60; PTS_EIIB; 1.
DR   Pfam; PF00358; PTS_EIIA_1; 1.
DR   Pfam; PF00367; PTS_EIIB; 1.
DR   Pfam; PF02378; PTS_EIIC; 1.
DR   ProDom; PD001476; Ptrans_EIIB; 1.
DR   ProDom; PD002243; PTS_EIIA; 1.
DR   TIGRFAMs; TIGR00830; PTBA; 1.
DR   PROSITE; PS51093; PTS_EIIA_TYPE_1; 1.
DR   PROSITE; PS00371; PTS_EIIA_TYPE_1_HIS; 1.
DR   PROSITE; PS51098; PTS_EIIB_TYPE_1; 1.
DR   PROSITE; PS01035; PTS_EIIB_TYPE_1_CYS; 1.
DR   PROSITE; PS51103; PTS_EIIC_TYPE_1; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Kinase; Membrane; Phosphotransferase system; Plasmid;
KW   Sugar transport; Transferase; Transmembrane; Transport.
FT   CHAIN         1    674       PTS system glucose-specific EIIBCA
FT                                component.
FT                                /FTId=PRO_0000186558.
FT   TRANSMEM    126    146       Potential.
FT   TRANSMEM    162    182       Potential.
FT   TRANSMEM    193    213       Potential.
FT   TRANSMEM    225    245       Potential.
FT   TRANSMEM    260    280       Potential.
FT   TRANSMEM    303    323       Potential.
FT   TRANSMEM    344    364       Potential.
FT   TRANSMEM    376    396       Potential.
FT   TRANSMEM    409    429       Potential.
FT   TRANSMEM    442    462       Potential.
FT   DOMAIN        1     89       PTS EIIB type-1.
FT   DOMAIN      117    476       PTS EIIC type-1.
FT   DOMAIN      542    646       PTS EIIA type-1.
FT   ACT_SITE     28     28       Phosphocysteine intermediate; for EIIB
FT                                activity (By similarity).
FT   ACT_SITE    594    594       Tele-phosphohistidine intermediate; for
FT                                EIIA activity (By similarity).
SQ   SEQUENCE   674 AA;  71626 MW;  67A75AAF76E42FA2 CRC64;
     MASKLTTTSQ HILENLGGPD NITSMTHCAT RLRFQVKDQS IVDQQEIDSD PSVLGVVPQG
     STGMQVVMGG SVANYYQEIL KLDGMKHFAD GEATESSSKK EYGGVRGKYS GIDYAFEFLS
     DTFRPILWAL LGASLIITLL VLADTFGLQD FRAPMDEQPD TYVFLHSMWR SVFYFLPIMV
     GATAARKLGA NEWIGAAIPA ALLTPEFLAL GSAGDTVTVF GLPMVLNDYS GQVFPPLIAA
     IGLYWVEKAL KKIIPEAVQM VFVPFFSLLI MIPATAFLLG PFGIGVGNGI SSLLEAVNNF
     SPFILSIVIP LLYPFLVPLG LHWPLNAIMI QNLNTLGYDF IQGPMGAWNF ACFGLVTGVF
     LIALKEKNRA MRQVSLGGML AGLLGGISEP SLYGVLLRFK KTYFRLLPGC LVGGIVMGIF
     DIKAYAFVFT SLLTIPAMDP WLGYTVGIAA AFFTSMLLVL FFDYRSDAER DEAKAQMAAA
     EQTNNTPAAP AAPVAPAAGA AAAGGAAGAT AVATKPRLAA GQLVEITSPL EGHAVPLSEV
     PDPIFAAGKL GPGIAIEPTG NTVVAPADAT VILVQKSGHA VALRLESGVE LLIHIGLDTV
     QLGGEGFKVH VERKQQVKAG DPLITFDPEF IRSKNLPLIT PVVVSNANKF GEIVGIEAAQ
     ADATTTVIKV NGAE
//