Reviewed, UniProtKB/Swiss-Prot Q45298 (PTG3B_CORGL)

Names and origin

Protein names

Recommended name:
    PTS system glucose-specific EIIBCA component
Alternative name(s):
    EIIBCA-Glc
    EII-Glc/EIII-Glc
Including the following 3 domains:
    1- Recommended name:
            Glucose-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system glucose-specific EIIB component
    2- Recommended name:
            Glucose permease IIC component
        Alternative name(s):
            PTS system glucose-specific EIIC component
    3- Recommended name:
            Glucose-specific phosphotransferase enzyme IIA component
              EC=2.7.1.-
        Alternative name(s):
            PTS system glucose-specific EIIA component

Gene names

Name:

ptsG

Encoded on

Plasmid pBSBG2

Organism

Corynebacterium glutamicum (Brevibacterium flavum)

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	674 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport By similarity.
Catalytic activity	Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine. Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Domain	The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain. The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
Sequence similarities	Contains 1 PTS EIIA type-1 domain. Contains 1 PTS EIIB type-1 domain. Contains 1 PTS EIIC type-1 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 674	674	PTS system glucose-specific EIIBCA component		PRO_0000186558
Regions
	Transmembrane	126 – 146	21	Potential
	Transmembrane	162 – 182	21	Potential
	Transmembrane	193 – 213	21	Potential
	Transmembrane	225 – 245	21	Potential
	Transmembrane	260 – 280	21	Potential
	Transmembrane	303 – 323	21	Potential
	Transmembrane	344 – 364	21	Potential
	Transmembrane	376 – 396	21	Potential
	Transmembrane	409 – 429	21	Potential
	Transmembrane	442 – 462	21	Potential
	Domain	1 – 89	89	PTS EIIB type-1
	Domain	117 – 476	360	PTS EIIC type-1
	Domain	542 – 646	105	PTS EIIA type-1
Sites
	Active site	28	1	Phosphocysteine intermediate; for EIIB activity By similarity
	Active site	594	1	Tele-phosphohistidine intermediate; for EIIA activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q45298-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 67A75AAF76E42FA2	FASTA	674	71,626
	10 20 30 40 50 60 MASKLTTTSQ HILENLGGPD NITSMTHCAT RLRFQVKDQS IVDQQEIDSD PSVLGVVPQG 70 80 90 100 110 120 STGMQVVMGG SVANYYQEIL KLDGMKHFAD GEATESSSKK EYGGVRGKYS GIDYAFEFLS 130 140 150 160 170 180 DTFRPILWAL LGASLIITLL VLADTFGLQD FRAPMDEQPD TYVFLHSMWR SVFYFLPIMV 190 200 210 220 230 240 GATAARKLGA NEWIGAAIPA ALLTPEFLAL GSAGDTVTVF GLPMVLNDYS GQVFPPLIAA 250 260 270 280 290 300 IGLYWVEKAL KKIIPEAVQM VFVPFFSLLI MIPATAFLLG PFGIGVGNGI SSLLEAVNNF 310 320 330 340 350 360 SPFILSIVIP LLYPFLVPLG LHWPLNAIMI QNLNTLGYDF IQGPMGAWNF ACFGLVTGVF 370 380 390 400 410 420 LIALKEKNRA MRQVSLGGML AGLLGGISEP SLYGVLLRFK KTYFRLLPGC LVGGIVMGIF 430 440 450 460 470 480 DIKAYAFVFT SLLTIPAMDP WLGYTVGIAA AFFTSMLLVL FFDYRSDAER DEAKAQMAAA 490 500 510 520 530 540 EQTNNTPAAP AAPVAPAAGA AAAGGAAGAT AVATKPRLAA GQLVEITSPL EGHAVPLSEV 550 560 570 580 590 600 PDPIFAAGKL GPGIAIEPTG NTVVAPADAT VILVQKSGHA VALRLESGVE LLIHIGLDTV 610 620 630 640 650 660 QLGGEGFKVH VERKQQVKAG DPLITFDPEF IRSKNLPLIT PVVVSNANKF GEIVGIEAAQ 670 ADATTTVIKV NGAE

References

[1]	"Cloning and nucleotide sequence of enzyme II of Brevibacterium lactofermentum phosphotransferase system." Yoon K.-H. Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13869 / DSMZ 1412 / NCIMB 9567.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L18875 Genomic DNA. Translation: AAA22992.1.
3D structure databases
HSSP	HSSP built from PDB template 1GLC based on UniProtKB P08837.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.1.69. 812.
Family and domain databases
InterPro	IPR018113. PTrfase_EIIB/Cys_phospho_site. IPR001127. PTS_EIIA_1_perm. IPR001996. PTS_EIIB. IPR003352. PTS_EIIC. IPR013013. PTS_EIIC_1. [Graphical view]
Gene3D	G3DSA:3.30.1360.60. PTS_EIIB. 1 hit.
Pfam	PF00358. PTS_EIIA_1. 1 hit. PF00367. PTS_EIIB. 1 hit. PF02378. PTS_EIIC. 1 hit. [Graphical view]
ProDom	PD001476. Ptrans_EIIB. 1 hit. PD002243. PTS_EIIA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00830. PTBA. 1 hit.
PROSITE	PS51093. PTS_EIIA_TYPE_1. 1 hit. PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit. PS51098. PTS_EIIB_TYPE_1. 1 hit. PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit. PS51103. PTS_EIIC_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTG3B_CORGL

Accession

Primary (citable) accession number: Q45298

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)