Reviewed, UniProtKB/Swiss-Prot Q45291 (GALE_CORGL)

Names and origin

Protein names

Recommended name:
    UDP-glucose 4-epimerase
    EC=5.1.3.2
Alternative name(s):
    UDP-galactose 4-epimerase
    Galactowaldenase

Gene names

Name:	galE
Ordered Locus Names:	Cgl1921, cg2104

Organism

Corynebacterium glutamicum (Brevibacterium flavum) [Complete proteome] [HAMAP]

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	UDP-glucose = UDP-galactose.
Cofactor	NAD.
Pathway	Carbohydrate metabolism; galactose metabolism.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the sugar epimerase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Galactose metabolism
Ligand	NAD
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	galactose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	UDP-glucose 4-epimerase activity Inferred from electronic annotation. Source: EC coenzyme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	UDP-glucose 4-epimerase		PRO_0000183202
Regions
	Nucleotide binding	2 – 33	32	NAD By similarity
Sites
	Active site	139	1	Proton acceptor By similarity
	Binding site	115	1	Substrate By similarity
Experimental info
	Sequence conflict	16	1	A → R in CAA89986. Ref.1
	Sequence conflict	61	1	S → L in CAA89986. Ref.1
	Sequence conflict	201	1	T → I in CAA89986. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q45291-1 [UniParc]. Last modified July 11, 2002. Version 2. Checksum: 3411E25C83DBC553	FASTA	329	35,263
	10 20 30 40 50 60 MKLLVTGGAG YVGSVAAAVL LEHGHDVTII DNFSTGNREA VPADARLIEG DVNDVVEEVL 70 80 90 100 110 120 SEGGFEGVVH FAARSLVGES VEKPNEYWHD NVVTALTLLD AMRAHGVNNL VFSSTAATYG 130 140 150 160 170 180 EPDVVPITED MPTQPTNAYG ATKLSIDYAI TSYAAAFGLA ATSLRYFNVA GAYGNIGENR 190 200 210 220 230 240 EVETHLIPLV LQVATGHREK TFMFGDDWPT PDGTAVRDYI HILDLAKAHV LALESNEAGK 250 260 270 280 290 300 HRIFNLGSGD GYSVKQVVEM CREVTGHPIP AEVAPRRAGD PATLIASSEK AKQELGWTPE 310 320 HTDLRTIVED AWAFTSALGD RSHAAKKKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The galE gene encoding the UDP-galactose 4-epimerase of Brevibacterium lactofermentum is coupled transcriptionally to the dmdR gene." Oguiza J.A., Marcos A.T., Malumbres M., Martin J.F. Gene 177:103-107(1996) [PubMed: 8921853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13869 / DSMZ 1412 / NCIMB 9567.
[2]	"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z49823 Genomic DNA. Translation: CAA89986.1. BA000036 Genomic DNA. Translation: BAB99314.1. BX927153 Genomic DNA. Translation: CAF20262.1.
PIR	JC5168.
RefSeq	NP_601127.1. YP_226163.1.
3D structure databases
HSSP	HSSP built from PDB template 1EK6 based on UniProtKB Q14376.
ModBase	Search...
Genome annotation databases
GeneID	1019878. 3343869.
GenomeReviews	Gene locus Cgl1921 in contig BA000036_GR. Gene locus cg2104 in contig BX927147_GR.
KEGG	cgb:cg2104. cgl:NCgl1846.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q45291.
OMA	Q45291. DPQVPTN.
Enzyme and pathway databases
BioCyc	CGLU196627-1:CG2104-MON.
BRENDA	5.1.3.2. 812.
Family and domain databases
InterPro	IPR001509. Epimerase_deHydtase. IPR005886. GalE. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR10366:SF39. GalE. 1 hit.
Pfam	PF01370. Epimerase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01179. galE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GALE_CORGL

Accession

Primary (citable) accession number: Q45291

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 11, 2002
Last modified:	June 16, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)