Q45220 (ISPA_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable farnesyl diphosphate synthase
Short name=FPP synthase
EC=2.5.1.10

Alternative name(s):
(2E,6E)-farnesyl diphosphate synthase
Geranyltranstransferase

Gene names

Name:	fppS
Ordered Locus Names:	blr2148

Organism

Bradyrhizobium japonicum

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate.
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the FPP/GGPP synthase family.
Sequence caution	The sequence BAC47413.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	geranyltranstransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	Probable farnesyl diphosphate synthase		PRO_0000123987
Sites
	Metal binding	114	1	Magnesium 1 By similarity
	Metal binding	114	1	Magnesium 2 By similarity
	Metal binding	120	1	Magnesium 1 By similarity
	Metal binding	120	1	Magnesium 2 By similarity
	Metal binding	253	1	Magnesium 3 By similarity
	Binding site	75	1	Isopentenyl diphosphate By similarity
	Binding site	78	1	Isopentenyl diphosphate By similarity
	Binding site	107	1	Isopentenyl diphosphate By similarity
	Binding site	125	1	Geranyl diphosphate By similarity
	Binding site	126	1	Isopentenyl diphosphate By similarity
	Binding site	208	1	Geranyl diphosphate By similarity
	Binding site	209	1	Geranyl diphosphate By similarity
	Binding site	250	1	Geranyl diphosphate By similarity
	Binding site	267	1	Geranyl diphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q45220 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 47644FAF0220CE4C	FASTA	332	34,639
	10 20 30 40 50 60 MQTGSTPHDD RAGVSANGIL GAQARGASGR LLPEIWMQDG AKRVEEALAR LLCAEDDGET 70 80 90 100 110 120 ELMAAMRYAT LHGGKRTRAL LCLAAGALAD TPAHMLDDVG AAIEMMHACT LVHDDLPAMD 130 140 150 160 170 180 DDVLRRGLPT VHVKFGEATA ILVGDALQAH AFLTLASLNA PGDSPIALVR ELAQAVSAEG 190 200 210 220 230 240 AAGGQAIDLS LVGKHVELDR IVAMHRMKSG ALVRASVRMG ALCAVGVNAA HAALYCALDH 250 260 270 280 290 300 YSACFGLALQ VIDDILDVTA DTAALGKTPG KDAAAQKPTC ASIMGLQEAR QFALDLLRDA 310 320 330 GEAIAPLGPR AERLAQLIQR ANAYLFKHAP RA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and mutagenesis of a cytochrome P-450 locus from Bradyrhizobium japonicum that is expressed anaerobically and symbiotically." Tully R.E., Keister D.L. Appl. Environ. Microbiol. 59:4136-4142(1993) [PubMed: 16349113] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: USDA 110.
[2]	"Identification and sequencing of a cytochrome P450 gene cluster from Bradyrhizobium japonicum." Tully R.E., van Berkum P., Lovins K.W., Keister D.L. Biochim. Biophys. Acta 1398:243-255(1998) [PubMed: 9655913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: USDA 110.
[3]	"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U12678 Genomic DNA. Translation: AAC28894.1. BA000040 Genomic DNA. Translation: BAC47413.1. Different initiation.
PIR	I40213.
RefSeq	NP_768788.1. NC_004463.1.
3D structure databases
ProteinModelPortal	Q45220.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1055401.
GenomeReviews	Gene locus blr2148 in contig BA000040_GR.
KEGG	bja:blr2148.
NMPDR	fig|224911.1.peg.2148.
PATRIC	21187616. VBIBraJap65052_2070.
Phylogenomic databases
HOGENOM	HBG732667.
OMA	MPIAPAL.
PhylomeDB	Q45220.
ProtClustDB	CLSK506592.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR2148-MONOMER.
Family and domain databases
InterPro	IPR000092. Polyprenyl_synt. IPR017446. Polyprenyl_synth-rel. IPR008949. Terpenoid_synth. [Graphical view]
Gene3D	G3DSA:1.10.600.10. Terpenoid_synth. 1 hit.
KO	K00795.
PANTHER	PTHR12001. Polyprenyl_synt. 1 hit.
Pfam	PF00348. polyprenyl_synt. 1 hit. [Graphical view]
SUPFAM	SSF48576. Terpenoid_synth. 1 hit.
PROSITE	PS00723. POLYPRENYL_SYNTHASE_1. 1 hit. PS00444. POLYPRENYL_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPA_BRAJA

Accession

Primary (citable) accession number: Q45220

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program