Q45093 (Q45093_BACCI) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Beta-galactosidase PIRNR PIRNR001084
Short name=Beta-gal PIRNR PIRNR001084
EC=3.2.1.23 PIRNR PIRNR001084

Gene names

Name:

bgaB EMBL AAA22260.1

Organism

Bacillus circulans EMBL AAA22260.1

Taxonomic identifier

1397 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	690 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. PIRNR PIRNR001084
Sequence similarities	Belongs to the glycosyl hydrolase 42 family. PIRNR PIRNR001084

Ontologies

Keywords
Ligand	Metal-binding PIRSR PIRSR001084-3 Zinc PIRSR PIRSR001084-3
Molecular function	Glycosidase PIRNR PIRNR001084 Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	beta-galactosidase complex Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	158	1	Proton donor By similarity PIRSR PIRSR001084-1
	Active site	315	1	Nucleophile By similarity PIRSR PIRSR001084-1
	Metal binding	123	1	Zinc By similarity PIRSR PIRSR001084-3
	Metal binding	163	1	Zinc By similarity PIRSR PIRSR001084-3
	Metal binding	165	1	Zinc By similarity PIRSR PIRSR001084-3
	Metal binding	168	1	Zinc By similarity PIRSR PIRSR001084-3
	Binding site	119	1	Substrate By similarity PIRSR PIRSR001084-2
	Binding site	157	1	Substrate By similarity PIRSR PIRSR001084-2
	Binding site	323	1	Substrate By similarity PIRSR PIRSR001084-2

Sequences

	Sequence		Length	Mass (Da)
	Q45093 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: FAA00B13B87A39D5	FASTA	690	78,245
	10 20 30 40 50 60 MTYKYSPVSS KVPRMLHGAD YNPEQWLRYP EVLEEDIRLM KLAKCNVMSI GIFSWVSLEP 70 80 90 100 110 120 EEGVYTFEWL DQVLDRFAAN GIYAFLATPS GARPAWMSAK YPEVLRVGAN RVRNLHGFRH 130 140 150 160 170 180 NHCYTSPVYR EKVTAINTKL AERYSDHPAV IGWHISNEFG GDCHCDYCQD AFRGWVKNKY 190 200 210 220 230 240 GTLDELNHSW WTTFWSHTVT DWSQVESPAP HGETQVHAMN LDWRRFVTDQ TADFIVHETK 250 260 270 280 290 300 PLKAKNPDLP VTTNLMEFYE GLNYWKFADI LDFLSWDSYP TWHDADEEDK LASRIAMMHD 310 320 330 340 350 360 IVRSIKGGQP FLLMESTPSS TNWQEVSKLK KPGMHLLSSL QAVAHGSDSV QYFQWRKSRG 370 380 390 400 410 420 SSEKLHGAVV DHVGTEHTRV FQDVTDVGTA LEGMEAIVGT SVPAEVGIIF DWENRWAVND 430 440 450 460 470 480 SQGPRNIGVK YEQTVEAHYE AFWKKGVAVD VIDMDADLSK YKLLVAPMLY LVREGVGERI 490 500 510 520 530 540 EQFVENGGTF VATYWSGIVN ENDLCFLGGF PGPLRKTLGI WSEEIDGLHD RDLNGVIPVK 550 560 570 580 590 600 GNALQLNAEY DAIELCDLIH LEGAEALATY RSDFYAGRPA LTVNRLGAGK AYYIATRTKA 610 620 630 640 650 660 PFYDDFYGSL IADLGIERAL ETQLPAGVTA HIRTDGTADY VFVQNYTPET KQVQLDEQSY 670 680 690 SDLLSGDMME GSLELQPYDI QVLRRATERK

References

[1]	"Two new beta-d-galactosidases from Bacillus circulans: A common sequence homology surrounding the putative active site region in several B-glycosidase families." Nelms J., Fotheringham I.G. Submitted (OCT-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: G1 EMBL AAA22260.1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L03425 Genomic DNA. Translation: AAA22260.1.
3D structure databases
HSSP	HSSP built from PDB template 1KWG based on UniProtKB O69315.
ProteinModelPortal	Q45093.
ModBase	Search...
Protein family/group databases
CAZy	GH42. Glycoside Hydrolase Family 42.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR013739. Beta_galactosidase_C. IPR013738. Beta_galactosidase_Trimer. IPR003476. Glyco_hydro_42. IPR013529. Glyco_hydro_42_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF02449. Glyco_hydro_42. 1 hit. PF08533. Glyco_hydro_42C. 1 hit. PF08532. Glyco_hydro_42M. 1 hit. [Graphical view]
PIRSF	PIRSF001084. B-galactosidase. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q45093_BACCI

Accession

Primary (citable) accession number: Q45093

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1996
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)