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Protein

Glucuronoxylanase XynC

Gene

xynC

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the depolymerization of methylglucuronoxylan (MeGAXn) from different sources. It cleaves the beta-1,4-xylosidic bond penultimate to that linking carbon one of the xylose residue substituted with alpha-1,2-linked 4-O-methyl-D-glucuronate (MeGA).1 Publication

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-xylosyl links in some glucuronoarabinoxylans.

Kineticsi

The Km value for methylglucuronoxylan from sweetgum is 1.63 mg/ml. The activity is directly correlated to the degree of substitution of the glucuronosyl moiety on the xylan chain.
    1. Vmax=59.5 µmol/min/mg enzyme with methylglucuronoxylan from sweetgum as substrate (at 37 degrees Celsius and pH 6)1 Publication

    pH dependencei

    Optimum pH is 6.1 Publication

    Temperature dependencei

    Optimum temperature is 65 degrees Celsius. Half the activity is retained for 25 hours at 40 degrees Celsius and for 5 hours at 50 degrees Celsius.1 Publication

    Pathwayi: xylan degradation

    This protein is involved in the pathway xylan degradation, which is part of Glycan degradation.
    View all proteins of this organism that are known to be involved in the pathway xylan degradation and in Glycan degradation.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei171Proton donor1 Publication1
    Active sitei260Nucleophile1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosidase, Hydrolase
    Biological processCarbohydrate metabolism, Polysaccharide degradation, Xylan degradation

    Enzyme and pathway databases

    BioCyciBSUB:BSU18150-MONOMER
    MetaCyc:BSU18150-MONOMER
    BRENDAi3.2.1.136 658
    SABIO-RKQ45070
    UniPathwayiUPA00114

    Protein family/group databases

    CAZyiGH30 Glycoside Hydrolase Family 30

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucuronoxylanase XynC (EC:3.2.1.136)
    Alternative name(s):
    Endoxylanase XynC
    Glucuronoxylan xylanohydrolase
    Gene namesi
    Name:xynC
    Synonyms:ynfF
    Ordered Locus Names:BSU18150
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    Proteomesi
    • UP000001570 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 32Sequence analysisAdd BLAST32
    ChainiPRO_000027864633 – 422Glucuronoxylanase XynCAdd BLAST390

    Proteomic databases

    PaxDbiQ45070
    PRIDEiQ45070

    Expressioni

    Inductioni

    Constitutively expressed.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100009996

    Structurei

    Secondary structure

    1422
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi36 – 46Combined sources11
    Beta strandi49 – 53Combined sources5
    Helixi56 – 59Combined sources4
    Helixi64 – 71Combined sources8
    Beta strandi82 – 87Combined sources6
    Helixi91 – 97Combined sources7
    Helixi98 – 106Combined sources9
    Beta strandi110 – 116Combined sources7
    Helixi120 – 122Combined sources3
    Beta strandi123 – 128Combined sources6
    Beta strandi131 – 137Combined sources7
    Helixi139 – 141Combined sources3
    Helixi142 – 158Combined sources17
    Beta strandi164 – 168Combined sources5
    Helixi183 – 192Combined sources10
    Helixi194 – 196Combined sources3
    Beta strandi198 – 208Combined sources11
    Helixi211 – 218Combined sources8
    Helixi221 – 225Combined sources5
    Beta strandi228 – 233Combined sources6
    Helixi239 – 241Combined sources3
    Helixi245 – 250Combined sources6
    Beta strandi255 – 261Combined sources7
    Helixi270 – 272Combined sources3
    Turni274 – 277Combined sources4
    Helixi278 – 289Combined sources12
    Beta strandi294 – 303Combined sources10
    Beta strandi306 – 308Combined sources3
    Beta strandi312 – 314Combined sources3
    Helixi316 – 325Combined sources10
    Beta strandi333 – 337Combined sources5
    Beta strandi345 – 352Combined sources8
    Beta strandi355 – 362Combined sources8
    Beta strandi364 – 366Combined sources3
    Beta strandi368 – 376Combined sources9
    Beta strandi379 – 388Combined sources10
    Beta strandi391 – 395Combined sources5
    Beta strandi405 – 410Combined sources6
    Beta strandi412 – 422Combined sources11

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3GTNX-ray2.68A/B33-422[»]
    3KL0X-ray1.64A/B/C/D33-422[»]
    3KL3X-ray2.33A/B/C/D33-422[»]
    3KL5X-ray2.59A/B/C/D33-422[»]
    ProteinModelPortaliQ45070
    SMRiQ45070
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ45070

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 30 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG4108MYR Bacteria
    ENOG410XT5H LUCA
    HOGENOMiHOG000245812
    InParanoidiQ45070
    KOiK15924
    OMAiAWIGDLT
    PhylomeDBiQ45070

    Family and domain databases

    Gene3Di2.60.40.1180, 2 hits
    InterProiView protein in InterPro
    IPR033452 GH30_C
    IPR001139 Glyco_hydro_30
    IPR013780 Glyco_hydro_b
    IPR017853 Glycoside_hydrolase_SF
    PANTHERiPTHR11069 PTHR11069, 1 hit
    PfamiView protein in Pfam
    PF17189 Glyco_hydro_30C, 1 hit
    SUPFAMiSSF51445 SSF51445, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q45070-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MIPRIKKTIC VLLVCFTMLS VMLGPGATEV LAASDVTVNV SAEKQVIRGF
    60 70 80 90 100
    GGMNHPAWAG DLTAAQRETA FGNGQNQLGF SILRIHVDEN RNNWYKEVET
    110 120 130 140 150
    AKSAVKHGAI VFASPWNPPS DMVETFNRNG DTSAKRLKYN KYAAYAQHLN
    160 170 180 190 200
    DFVTFMKNNG VNLYAISVQN EPDYAHEWTW WTPQEILRFM RENAGSINAR
    210 220 230 240 250
    VIAPESFQYL KNLSDPILND PQALANMDIL GTHLYGTQVS QFPYPLFKQK
    260 270 280 290 300
    GAGKDLWMTE VYYPNSDTNS ADRWPEALDV SQHIHNAMVE GDFQAYVWWY
    310 320 330 340 350
    IRRSYGPMKE DGTISKRGYN MAHFSKFVRP GYVRIDATKN PNANVYVSAY
    360 370 380 390 400
    KGDNKVVIVA INKSNTGVNQ NFVLQNGSAS NVSRWITSSS SNLQPGTNLT
    410 420
    VSGNHFWAHL PAQSVTTFVV NR
    Length:422
    Mass (Da):47,337
    Last modified:November 1, 1996 - v1
    Checksum:iFEEB750E3B9DAAB8
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z73234 Genomic DNA Translation: CAA97612.1
    AL009126 Genomic DNA Translation: CAB13698.1
    PIRiE69892
    RefSeqiNP_389697.1, NC_000964.3
    WP_003231534.1, NZ_JNCM01000035.1

    Genome annotation databases

    EnsemblBacteriaiCAB13698; CAB13698; BSU18150
    GeneIDi937052
    KEGGibsu:BSU18150
    PATRICifig|224308.179.peg.1979

    Similar proteinsi

    Entry informationi

    Entry nameiXYNC_BACSU
    AccessioniPrimary (citable) accession number: Q45070
    Secondary accession number(s): Q796G4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
    Last sequence update: November 1, 1996
    Last modified: May 23, 2018
    This is version 106 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health