Q45066 (PARC_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA topoisomerase 4 subunit A
EC=5.99.1.-

Alternative name(s):
Topoisomerase IV subunit A

Gene names

Name:	parC
Synonyms:	grlA
Ordered Locus Names:	BSU18100

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	806 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule By similarity.
Subunit structure	Composed of two subunits: ParC and ParE.
Subcellular location	Cytoplasm. Note: Bipolarly localized.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	DNA-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 806	806	DNA topoisomerase 4 subunit A		PRO_0000145394
Sites
	Active site	121	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Experimental info
	Sequence conflict	210	1	V → L in CAA97607. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q45066 [UniParc]. Last modified June 16, 2009. Version 2. Checksum: 2CFC919CFC7D0BAF	FASTA	806	91,321
	10 20 30 40 50 60 MSQPELFHDL PLEEVIGDRF GRYSKYIIQD RALPDARDGL KPVQRRILYA MHTDGNTFDK 70 80 90 100 110 120 NFRKAAKTVG NVIGNYHPHG DSSVYEAMVR MSQDWKVRNV LIEMHGNNGS IDGDPPAAMR 130 140 150 160 170 180 YTEARLSPIA SELLRDIDKN TVEFVPNFDD TSKEPVVLPA MFPNLLVNGS TGISAGYATD 190 200 210 220 230 240 IPPHHLGEVI DAVIKRIQMP SCSVDELMEV IKGPDFPTGG IIQGVDGIRK AYETGKGKII 250 260 270 280 290 300 IRGKAEIETI RGGREQIVIT EIPFEVNKAN LVKKMDEFRI DKKVEGISEV RDETDRTGLR 310 320 330 340 350 360 VVIELKKEAD AKGILNFLYK NTDLQITYNF NMVAIHNRRP MLMSLPSILD AYIGHQKEVV 370 380 390 400 410 420 TNRSVYELQK AKDRHHIVEG LMKALSILDE VIATIRSSSD KRDAKNNLIA KYEFTEPQAE 430 440 450 460 470 480 AIVSLQLYRL TNTDITALKE EAEELGKKIE ELESILSNDK KLLKVITNSL KALKKKYADT 490 500 510 520 530 540 RRSVIEEKIE EIKINLEVMV ASEDVYVTVT KDGYLKRTSQ RSFAASNGQD FGMKDTDRML 550 560 570 580 590 600 HQFEMNTTDV LLLFTNKGSY IYCPVHQLPD IRWKDMGQHF SNLITIDRDE TIVKAIPIKE 610 620 630 640 650 660 FDPSAYLLFF TKNGMVKKTE LTHYKAQRYS KALVALNLKG EDELIDVHVT NGESQIFMAT 670 680 690 700 710 720 HLGYGLWFGE DEVNVVGARA AGVKGINLKE DDFVVSGEIL QQSDSIVLFT QRGAVKRMSL 730 740 750 760 770 780 SEFEKTSRAK RGVVMLRELK KNPHRVVALF ACGLEQRLMA ETEKGDRKEL QTKELRTNDR 790 800 YSNGSFFFDE EESGKVTAVW RLHTEQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"New genes in the 170 degrees region of the Bacillus subtilis genome encode DNA gyrase subunits, a thioredoxin, a xylanase and an amino acid transporter." Rose M., Entian K.-D. Microbiology 142:3097-3101(1996) [PubMed: 8969507] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"Bipolar localization of Bacillus subtilis topoisomerase IV, an enzyme required for chromosome segregation." Huang W.M., Libbey J.L., van de Hoeven P., Yu S.X. Proc. Natl. Acad. Sci. U.S.A. 95:4652-4657(1998) [PubMed: 9539793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / CB10.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 210.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z73234 Genomic DNA. Translation: CAA97607.1. AF024713 Genomic DNA. Translation: AAB81615.1. AL009126 Genomic DNA. Translation: CAB13693.2.
PIR	B69637.
RefSeq	NP_389692.2. NC_000964.3.
3D structure databases
ProteinModelPortal	Q45066.
SMR	Q45066. Positions 32-491, 499-805.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000001040; EBBACP00000001040; EBBACG00000001038.
GeneID	937107.
GenomeReviews	Gene locus BSU18100 in contig AL009126_GR.
KEGG	bsu:BSU18100.
NMPDR	fig|224308.1.peg.1813.
PATRIC	18975451. VBIBacSub10457_1917.
Organism-specific databases
GenoList	BSU18100. [Micado]
Phylogenomic databases
GeneTree	EBGT00050000002933.
HOGENOM	HBG635183.
PhylomeDB	Q45066.
ProtClustDB	PRK05561.
Enzyme and pathway databases
BioCyc	BSUB:BSU18100-MONOMER.
Family and domain databases
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013760. Topo_IIA_cen. IPR005741. TopoIV_A_Gpos. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 2 hits.
KO	K02621.
Pfam	PF03989. DNA_gyraseA_C. 5 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit. TIGR01061. ParC_Gpos. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PARC_BACSU

Accession

Primary (citable) accession number: Q45066
Secondary accession number(s): O30998

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 16, 2009
Last modified:	January 25, 2012
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program