Reviewed, UniProtKB/Swiss-Prot Q44951 (SYM_BORBU)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	BB_0587

Organism

Borrelia burgdorferi (Lyme disease spirochete) [Complete proteome] [HAMAP]

Taxonomic identifier

139 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	734 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_00098
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00098
Subunit structure	Homodimer By similarity. HAMAP MF_00098
Subcellular location	Cytoplasm HAMAP MF_00098.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 734	734	Methionyl-tRNA synthetase HAMAP MF_00098		PRO_0000139107
Regions
	Domain	570 – 675	106	tRNA-binding
	Motif	12 – 22	11	"HIGH" region HAMAP MF_00098
	Motif	330 – 334	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	143	1	Zinc By similarity
	Metal binding	146	1	Zinc By similarity
	Metal binding	155	1	Zinc By similarity
	Metal binding	158	1	Zinc By similarity
	Binding site	333	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q44951-1 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 585508811CED9CFB	FASTA	734	85,215
	10 20 30 40 50 60 MKKMNLVTAA LPYVNNIPHL GNLVQVLSAD AFARYSKMSG IETLYVCGTD EYGTATETKA 70 80 90 100 110 120 LIENTTPLEL CNKYYEIHKS IYKWFNIEFD IFGRTTNKNH QDIVQNFFLQ LEKNGYIKER 130 140 150 160 170 180 ETEQFYCNKD SMFLADRYVI GECPECQSMA KGDQCDNCSK LLNPTDLINP KCIICKNKPI 190 200 210 220 230 240 LKKTNHLYLD LPKIKTKLEK WIKNPDTSKN WNTNALKMTK AFLRDGLKER AITRDLKWGI 250 260 270 280 290 300 PVPKKGFENK VFYVWFDAPI GYISITKNII KNWESWWKNN DQVNLVQFIG KDNILFHTII 310 320 330 340 350 360 FPCIEIGSEE NWTILNQLSS SEYLNYENLK FSKSEGTGIF GNDAITTGIP SDIWRFYIYY 370 380 390 400 410 420 NRPEKSDFQF MWQDLMERVN TELIDNFSNL VNRVLTFQRK FFGDVIETIE IQNKFWKQIT 430 440 450 460 470 480 PKYNKILNLF KKTELKSALK EILKISSLGN KIFQDNEPWK RKNNSPQETK ELISNLIYLI 490 500 510 520 530 540 RDLSILMMPF IPETSKKIQQ FFGNSYQFST KILGTKSGIK KIEFTEILFN KLEQKKINNL 550 560 570 580 590 600 KLKYSGDKNM KENEQAENLP IAKEQPENLF REKVLLRVVK INKIERNPEA KNLFILKLDD 610 620 630 640 650 660 GTNKDKQIVS GLEGYYTEEE LLGKHIIIVD NLKPAKFRGI KSEGMLIAAE DKNKNFKVII 670 680 690 700 710 720 VEDSIQNPIA GERIILENDQ NKDLACPPKI DINKFLKANI VAENGELKIN GINLILENSK 730 NKILSKDIPN GTVC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi." Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L., Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B., Smith H.O., Venter J.C. Nature 390:580-586(1997) [PubMed: 9403685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.
[2]	"Conservation of gene arrangement and an unusual organization of rRNA genes in the linear chromosomes of the Lyme disease spirochaetes Borrelia burgdorferi, B. garinii and B. afzelii." Ojaimi C., Davidson B.E., Saint-Girons I., Old I.G. Microbiology 140:2931-2940(1994) [PubMed: 7812434] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 246-360. Strain: 212.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000783 Genomic DNA. Translation: AAB91520.1. L32146 Genomic DNA. Translation: AAC41404.1.
PIR	B70173.
RefSeq	NP_212721.1.
3D structure databases
SMR	Q44951. Positions 5-537, 434-652, 566-733.
ModBase	Search...
Genome annotation databases
GeneID	1195434.
GenomeReviews	Gene locus BB_0587 in contig AE000783_GR.
KEGG	bbu:BB0587.
NMPDR	fig|224326.1.peg.971.
TIGR	BB_0587.
Phylogenomic databases
HOGENOM	HBG721667.
OMA	SAYQPEQ.
PhylomeDB	Q44951.
Enzyme and pathway databases
BioCyc	BBUR224326:BB_0587-MONOMER.
BRENDA	6.1.1.10. 142596.
Family and domain databases
HAMAP	MF_00098. Met_tRNA_synth_type1. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR014729. Rossmann-like_a/b/a_fold. IPR002547. tRNA-bd_dom. IPR014758. tRNA-synt_met_N. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit. TIGR00399. metG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_BORBU

Accession

Primary (citable) accession number: Q44951

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	December 15, 1998
Last modified:	February 9, 2010
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)