Reviewed, UniProtKB/Swiss-Prot Q44737 (CHEA_BORBU)

Names and origin

Protein names

Recommended name:
    Chemotaxis protein cheA
    EC=2.7.13.3

Gene names

Name:	cheA
Ordered Locus Names:	BB_0669

Organism

Borrelia burgdorferi (Lyme disease spirochete) [Complete proteome] [HAMAP]

Taxonomic identifier

139 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	864 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. CheA is autophosphorylated; it can transfer its phosphate group to either cheB or cheY By similarity.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Contains 1 cheW-like domain. Contains 1 histidine kinase domain. Contains 1 HPt domain.

Ontologies

Keywords
Biological process	Chemotaxis Two-component regulatory system
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell motion Inferred from electronic annotation. Source: InterPro chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 864	864	Chemotaxis protein cheA		PRO_0000074712
Regions
	Domain	1 – 108	108	HPt
	Domain	480 – 725	246	Histidine kinase
	Domain	727 – 864	138	CheW-like
Amino acid modifications
	Modified residue	51	1	Phosphohistidine; by autocatalysis By similarity
Experimental info
	Sequence conflict	11	1	L → I in AAB96835. Ref.1
	Sequence conflict	182	1	S → H in AAB96835. Ref.1
	Sequence conflict	187	1	S → G in AAB96835. Ref.1
	Sequence conflict	239	1	L → S in AAB96835. Ref.1
	Sequence conflict	362	1	A → S in AAB96835. Ref.1
	Sequence conflict	559	1	S → P in AAB96835. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q44737-1 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: C1111DE0877BE624	FASTA	864	98,353
	10 20 30 40 50 60 MEILDLENEE LLGVFFEEAQ NLVDILEENI MSLEDDPNNS DTIDEIFRAA HTLKGSSASL 70 80 90 100 110 120 DMMELSDFTH IVEDVFDAIR DGKVNINNDL VDLLLSSLDV IKEMLALRID GKVYLNDISD 130 140 150 160 170 180 LKSKLKQFLV IDDQTFIKRF DGNSIKNNFC LSESDLEEIR EGLGIGQKVL RISVVFNSNS 190 200 210 220 230 240 NSEVENSGLK IFNILKNLGS VLHTIPKYEQ IIEDKFLKRV DYYLIYSDIE GVKKSLDSLN 250 260 270 280 290 300 LIESYLVDEF NVKEELKKLA DEEIKDVDLD SNFVLNDNFD FTEDEISDLL LEVENQKLFK 310 320 330 340 350 360 VRLDFVKDNP MATISGLQML QALKSLGKIF KSIPDSSELL ADKFFDFVIY YLISNTSEES 370 380 390 400 410 420 IAKKINLPDV VSHFEIKNVN LESLKSVRLK EDDEAPFKEN KNIKKNSPIS VNLIRIDSKK 430 440 450 460 470 480 IDYILNLVSE AVISKSSYNQ INSEMITLFY NFNYFYDYQE SFQRNFLIDL KIVFKDAGLT 490 500 510 520 530 540 LEDEIESHIN SLMSFKMEKA LKDISELRNS FFRLLQNFKM TSGRLSRIIT DLHESVLKTR 550 560 570 580 590 600 MLPISNIFSR FTRVVRDLSK KLNKIVNLKM EGEETELDKS VIDDLVDPLM HCVRNSMDHG 610 620 630 640 650 660 LETVEERVKR GKSKAGTIIL RAKNEGNVIS IEIEDDGIGI DPKVIRRKLI EKGTIKEDAI 670 680 690 700 710 720 YSDFELINLI FAPGFSTAVQ VTDLSGRGVG LDVVKKSIEK LNGTILVESE IGLGTIFKIK 730 740 750 760 770 780 LPLTLVIIQG LLVKSGSETY VIPLNNVLET HRITEHDIKL LENYHEVYNL RDEVISVLRL 790 800 810 820 830 840 DKLFNITRDD SLIEKFLIVV NTSNMKIAIV VDSILGEEDF VVKPIKDKFS SSAGIVGATT 850 860 LGNGKVVLII DVFKLFDLQK DTKE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A cheA cheW operon in Borrelia burgdorferi, the agent of Lyme disease." Trueba G.A., Old I.G., Saint-Girons I., Johnson R.C. Res. Microbiol. 148:191-200(1997) [PubMed: 9765799] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 212.
[2]	"Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi." Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L., Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B., Smith H.O., Venter J.C. Nature 390:580-586(1997) [PubMed: 9403685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.
[3]	"An unexpected flaA homolog is present and expressed in Borrelia burgdorferi." Ge Y., Charon N.W. J. Bacteriol. 179:552-556(1997) [PubMed: 8990312] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30. Strain: 212.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U28962 Genomic DNA. Translation: AAB96835.1. AE000783 Genomic DNA. Translation: AAC67024.1. U62900 Genomic DNA. Translation: AAC44771.1. X91907 Genomic DNA. Translation: CAA63002.1.
PIR	D70183.
RefSeq	NP_212803.1.
3D structure databases
HSSP	HSSP built from PDB template 1I59 based on UniProtKB Q56310.
ModBase	Search...
Genome annotation databases
GeneID	1195521.
GenomeReviews	Gene locus BB_0669 in contig AE000783_GR.
KEGG	bbu:BB0669.
NMPDR	fig|224326.1.peg.1053.
TIGR	BB_0669.
Phylogenomic databases
HOGENOM	Q44737.
OMA	Q44737. SHEVHIT.
Enzyme and pathway databases
BioCyc	BBUR224326:BB_0669-MON.
BRENDA	2.7.13.3. 142596.
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR002545. CheW. IPR004358. Sig_transdc_His_kin-like_C. IPR008207. Sig_transdc_His_kin_P-trf. IPR004105. Sig_transdc_His_kin_subgr_dim. IPR005467. Sig_transdc_His_kinase_core. IPR010808. Sig_transdc_His_kinase_P2-bd. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF01584. CheW. 1 hit. PF02895. H-kinase_dim. 1 hit. PF02518. HATPase_c. 1 hit. PF01627. Hpt. 1 hit. PF07194. P2. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
ProDom	PD003142. Hpt_N. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00260. CheW. 1 hit. SM00387. HATPase_c. 1 hit. SM00073. HPT. 1 hit. [Graphical view]
PROSITE	PS50851. CHEW. 1 hit. PS50109. HIS_KIN. 1 hit. PS50894. HPT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEA_BORBU

Accession

Primary (citable) accession number: Q44737
Secondary accession number(s): P70857, Q44877

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 15, 1998
Last modified:	June 16, 2009
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)