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Q44696

- TRPG_BUCAI

UniProt

Q44696 - TRPG_BUCAI

Protein

Anthranilate synthase component 2

Gene

trpG

more
Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 2 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia By similarity.By similarity

    Catalytic activityi

    Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei84 – 841For GATase activityPROSITE-ProRule annotation
    Binding sitei84 – 841GlutamineBy similarity
    Binding sitei88 – 881GlutamineBy similarity
    Active sitei170 – 1701For GATase activityPROSITE-ProRule annotation
    Active sitei172 – 1721For GATase activityPROSITE-ProRule annotation

    GO - Molecular functioni

    1. anthranilate synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine metabolic process Source: UniProtKB-KW
    2. tryptophan biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

    Enzyme and pathway databases

    BioCyciBAPH107806:GBZJ-617-MONOMER.
    BAPH107806:GBZJ-619-MONOMER.
    UniPathwayiUPA00035; UER00040.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Anthranilate synthase component 2 (EC:4.1.3.27)
    Short name:
    AS
    Short name:
    ASII
    Alternative name(s):
    Anthranilate synthase, GATase component
    Anthranilate synthase, glutamine amidotransferase component
    Gene namesi
    Name:trpG
    Ordered Locus Names:BUpT02
    AND
    Name:trpG2
    Ordered Locus Names:BUpT04
    Encoded oniPlasmid pTrp (pBAp)0 Publication
    OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
    Taxonomic identifieri107806 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000001806: Plasmid pTrp

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 200200Anthranilate synthase component 2PRO_0000056872Add
    BLAST

    Interactioni

    Subunit structurei

    Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).By similarity

    Protein-protein interaction databases

    STRINGi107806.BUpT04.

    Structurei

    3D structure databases

    ProteinModelPortaliQ44696.
    SMRiQ44696. Positions 2-193.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 196194Glutamine amidotransferase type-1PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni57 – 593Glutamine bindingBy similarity
    Regioni134 – 1352Glutamine bindingBy similarity

    Sequence similaritiesi

    Contains 1 glutamine amidotransferase type-1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    HOGENOMiHOG000025029.
    KOiK01658.
    OMAiANPMPVA.
    OrthoDBiEOG6M9F11.

    Family and domain databases

    Gene3Di3.40.50.880. 1 hit.
    InterProiIPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR006221. TrpG/PapA_dom.
    [Graphical view]
    PfamiPF00117. GATase. 1 hit.
    [Graphical view]
    SUPFAMiSSF52317. SSF52317. 1 hit.
    TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
    PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q44696-1 [UniParc]FASTAAdd to Basket

    « Hide

    MANILLLDNI DSFTYNLVEQ LRNQKNNVLV YRNTVSIDII FNSLKKLTHP    50
    ILMLSPGPSL PKHAGCMLDL IKKVKGDIPI VGICLGHQAI VEAYGGIIGY 100
    AGEIFHGKAS LIRHDGLEMF EGVPQPLPVA RYHSLICNKI PEKFVINSYF 150
    EKMIMSVRNN CDRVCGFQFH PESILTTHGD QILEKIIHWA SLKYITNKKQ 200
    Length:200
    Mass (Da):22,569
    Last modified:December 1, 2000 - v2
    Checksum:iFF16C661D5887B04
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti69 – 691D → N in AAD09347. (PubMed:8642610)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43555 Genomic DNA. Translation: AAD09347.1.
    AY444795 Genomic DNA. Translation: AAS02054.1.
    AP001070 Genomic DNA. Translation: BAA95418.1.
    AP001070 Genomic DNA. Translation: BAA95419.1.
    RefSeqiNP_057963.1. NC_002252.1.
    NP_057964.1. NC_002252.1.
    WP_010892290.1. NC_002252.1.

    Genome annotation databases

    EnsemblBacteriaiBAA95418; BAA95418; BAA95418.
    BAA95419; BAA95419; BAA95419.
    GeneIDi1109446.
    1109447.
    KEGGibuc:BUpT02.
    buc:BUpT04.
    PATRICi21243480. VBIBucAph127364_0002.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43555 Genomic DNA. Translation: AAD09347.1 .
    AY444795 Genomic DNA. Translation: AAS02054.1 .
    AP001070 Genomic DNA. Translation: BAA95418.1 .
    AP001070 Genomic DNA. Translation: BAA95419.1 .
    RefSeqi NP_057963.1. NC_002252.1.
    NP_057964.1. NC_002252.1.
    WP_010892290.1. NC_002252.1.

    3D structure databases

    ProteinModelPortali Q44696.
    SMRi Q44696. Positions 2-193.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 107806.BUpT04.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA95418 ; BAA95418 ; BAA95418 .
    BAA95419 ; BAA95419 ; BAA95419 .
    GeneIDi 1109446.
    1109447.
    KEGGi buc:BUpT02.
    buc:BUpT04.
    PATRICi 21243480. VBIBucAph127364_0002.

    Phylogenomic databases

    HOGENOMi HOG000025029.
    KOi K01658.
    OMAi ANPMPVA.
    OrthoDBi EOG6M9F11.

    Enzyme and pathway databases

    UniPathwayi UPA00035 ; UER00040 .
    BioCyci BAPH107806:GBZJ-617-MONOMER.
    BAPH107806:GBZJ-619-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.880. 1 hit.
    InterProi IPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR006221. TrpG/PapA_dom.
    [Graphical view ]
    Pfami PF00117. GATase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52317. SSF52317. 1 hit.
    TIGRFAMsi TIGR00566. trpG_papA. 1 hit.
    PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The tryptophan biosynthetic pathway of aphid endosymbionts (Buchnera): genetics and evolution of plasmid-associated anthranilate synthase (trpEG) within the aphididae."
      Rouhbakhsh D., Lai C.-Y., von Dohlen C.D., Clark M.A., Baumann L., Baumann P., Moran N.A., Voegtlin D.J.
      J. Mol. Evol. 42:414-421(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Genetic mechanisms for regulating nutrient provisioning by symbionts to hosts: the pTrp plasmid in Buchnera."
      Plague G.R., Kaur S., Wilcox J.L., Bishop B., Moran N.A.
      Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: APS.
    3. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
      Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
      Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: APS.

    Entry informationi

    Entry nameiTRPG_BUCAI
    AccessioniPrimary (citable) accession number: Q44696
    Secondary accession number(s): Q5GL25, Q9K2G8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Plasmid, Reference proteome

    Documents

    1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
      Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3