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Protein

Anthranilate synthase component 2

Gene

trpG

more
Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia (By similarity).By similarity

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei84 – 841For GATase activityPROSITE-ProRule annotation
Binding sitei84 – 841GlutamineBy similarity
Binding sitei88 – 881GlutamineBy similarity
Active sitei170 – 1701For GATase activityPROSITE-ProRule annotation
Active sitei172 – 1721For GATase activityPROSITE-ProRule annotation

GO - Molecular functioni

  1. anthranilate synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine metabolic process Source: UniProtKB-KW
  2. tryptophan biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-617-MONOMER.
BAPH107806:GBZJ-619-MONOMER.
UniPathwayiUPA00035; UER00040.

Protein family/group databases

MEROPSiC26.960.

Names & Taxonomyi

Protein namesi
Recommended name:
Anthranilate synthase component 2 (EC:4.1.3.27)
Short name:
AS
Short name:
ASII
Alternative name(s):
Anthranilate synthase, GATase component
Anthranilate synthase, glutamine amidotransferase component
Gene namesi
Name:trpG
Ordered Locus Names:BUpT02
AND
Name:trpG2
Ordered Locus Names:BUpT04
Encoded oniPlasmid pTrp (pBAp)0 Publication
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Plasmid pTrp

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 200200Anthranilate synthase component 2PRO_0000056872Add
BLAST

Interactioni

Subunit structurei

Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).By similarity

Protein-protein interaction databases

STRINGi107806.BUpT04.

Structurei

3D structure databases

ProteinModelPortaliQ44696.
SMRiQ44696. Positions 2-193.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 196194Glutamine amidotransferase type-1PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni57 – 593Glutamine bindingBy similarity
Regioni134 – 1352Glutamine bindingBy similarity

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

HOGENOMiHOG000025029.
KOiK01658.
OMAiKVERAPF.
OrthoDBiEOG6M9F11.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q44696-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANILLLDNI DSFTYNLVEQ LRNQKNNVLV YRNTVSIDII FNSLKKLTHP
60 70 80 90 100
ILMLSPGPSL PKHAGCMLDL IKKVKGDIPI VGICLGHQAI VEAYGGIIGY
110 120 130 140 150
AGEIFHGKAS LIRHDGLEMF EGVPQPLPVA RYHSLICNKI PEKFVINSYF
160 170 180 190 200
EKMIMSVRNN CDRVCGFQFH PESILTTHGD QILEKIIHWA SLKYITNKKQ
Length:200
Mass (Da):22,569
Last modified:December 1, 2000 - v2
Checksum:iFF16C661D5887B04
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti69 – 691D → N in AAD09347 (PubMed:8642610).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43555 Genomic DNA. Translation: AAD09347.1.
AY444795 Genomic DNA. Translation: AAS02054.1.
AP001070 Genomic DNA. Translation: BAA95418.1.
AP001070 Genomic DNA. Translation: BAA95419.1.
RefSeqiNP_057963.1. NC_002252.1.
NP_057964.1. NC_002252.1.
WP_010892290.1. NC_002252.1.

Genome annotation databases

EnsemblBacteriaiBAA95418; BAA95418; BAA95418.
BAA95419; BAA95419; BAA95419.
GeneIDi1109446.
1109447.
KEGGibuc:BUpT02.
buc:BUpT04.
PATRICi21243480. VBIBucAph127364_0002.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43555 Genomic DNA. Translation: AAD09347.1.
AY444795 Genomic DNA. Translation: AAS02054.1.
AP001070 Genomic DNA. Translation: BAA95418.1.
AP001070 Genomic DNA. Translation: BAA95419.1.
RefSeqiNP_057963.1. NC_002252.1.
NP_057964.1. NC_002252.1.
WP_010892290.1. NC_002252.1.

3D structure databases

ProteinModelPortaliQ44696.
SMRiQ44696. Positions 2-193.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi107806.BUpT04.

Protein family/group databases

MEROPSiC26.960.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA95418; BAA95418; BAA95418.
BAA95419; BAA95419; BAA95419.
GeneIDi1109446.
1109447.
KEGGibuc:BUpT02.
buc:BUpT04.
PATRICi21243480. VBIBucAph127364_0002.

Phylogenomic databases

HOGENOMiHOG000025029.
KOiK01658.
OMAiKVERAPF.
OrthoDBiEOG6M9F11.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.
BioCyciBAPH107806:GBZJ-617-MONOMER.
BAPH107806:GBZJ-619-MONOMER.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The tryptophan biosynthetic pathway of aphid endosymbionts (Buchnera): genetics and evolution of plasmid-associated anthranilate synthase (trpEG) within the aphididae."
    Rouhbakhsh D., Lai C.-Y., von Dohlen C.D., Clark M.A., Baumann L., Baumann P., Moran N.A., Voegtlin D.J.
    J. Mol. Evol. 42:414-421(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genetic mechanisms for regulating nutrient provisioning by symbionts to hosts: the pTrp plasmid in Buchnera."
    Plague G.R., Kaur S., Wilcox J.L., Bishop B., Moran N.A.
    Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: APS.
  3. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiTRPG_BUCAI
AccessioniPrimary (citable) accession number: Q44696
Secondary accession number(s): Q5GL25, Q9K2G8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: December 1, 2000
Last modified: February 4, 2015
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.