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Q44696 (TRPG_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Anthranilate synthase component 2

Short name=AS
Short name=ASII
EC=4.1.3.27
Alternative name(s):
Anthranilate synthase, GATase component
Anthranilate synthase, glutamine amidotransferase component
Gene names
Name:trpG
Ordered Locus Names:BUpT02
AND
Name:trpG2
Ordered Locus Names:BUpT04
Encoded onPlasmid pTrp (pBAp)
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length200 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia By similarity.

Catalytic activity

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Pathway

Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.

Subunit structure

Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE) By similarity.

Sequence similarities

Contains 1 glutamine amidotransferase type-1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 200200Anthranilate synthase component 2
PRO_0000056872

Regions

Domain3 – 196194Glutamine amidotransferase type-1
Region57 – 593Glutamine binding By similarity
Region134 – 1352Glutamine binding By similarity

Sites

Active site841For GATase activity By similarity
Active site1701For GATase activity By similarity
Active site1721For GATase activity By similarity
Binding site841Glutamine By similarity
Binding site881Glutamine By similarity

Experimental info

Sequence conflict691D → N in AAD09347. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q44696 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: FF16C661D5887B04

FASTA20022,569
        10         20         30         40         50         60 
MANILLLDNI DSFTYNLVEQ LRNQKNNVLV YRNTVSIDII FNSLKKLTHP ILMLSPGPSL 

        70         80         90        100        110        120 
PKHAGCMLDL IKKVKGDIPI VGICLGHQAI VEAYGGIIGY AGEIFHGKAS LIRHDGLEMF 

       130        140        150        160        170        180 
EGVPQPLPVA RYHSLICNKI PEKFVINSYF EKMIMSVRNN CDRVCGFQFH PESILTTHGD 

       190        200 
QILEKIIHWA SLKYITNKKQ 

« Hide

References

« Hide 'large scale' references
[1]"The tryptophan biosynthetic pathway of aphid endosymbionts (Buchnera): genetics and evolution of plasmid-associated anthranilate synthase (trpEG) within the aphididae."
Rouhbakhsh D., Lai C.-Y., von Dohlen C.D., Clark M.A., Baumann L., Baumann P., Moran N.A., Voegtlin D.J.
J. Mol. Evol. 42:414-421(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Genetic mechanisms for regulating nutrient provisioning by symbionts to hosts: the pTrp plasmid in Buchnera."
Plague G.R., Kaur S., Wilcox J.L., Bishop B., Moran N.A.
Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: APS.
[3]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L43555 Genomic DNA. Translation: AAD09347.1.
AY444795 Genomic DNA. Translation: AAS02054.1.
AP001070 Genomic DNA. Translation: BAA95418.1.
AP001070 Genomic DNA. Translation: BAA95419.1.
RefSeqNP_057963.1. NC_002252.1.
NP_057964.1. NC_002252.1.

3D structure databases

ProteinModelPortalQ44696.
SMRQ44696. Positions 2-193.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING107806.BUpT04.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA95418; BAA95418; BAA95418.
BAA95419; BAA95419; BAA95419.
GeneID1109446.
1109447.
KEGGbuc:BUpT02.
buc:BUpT04.
PATRIC21243480. VBIBucAph127364_0002.

Phylogenomic databases

HOGENOMHOG000025029.
KOK01658.
OMAANPMPVA.
OrthoDBEOG6M9F11.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-617-MONOMER.
BAPH107806:GBZJ-619-MONOMER.
UniPathwayUPA00035; UER00040.

Family and domain databases

Gene3D3.40.50.880. 1 hit.
InterProIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamPF00117. GATase. 1 hit.
[Graphical view]
SUPFAMSSF52317. SSF52317. 1 hit.
TIGRFAMsTIGR00566. trpG_papA. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRPG_BUCAI
AccessionPrimary (citable) accession number: Q44696
Secondary accession number(s): Q5GL25, Q9K2G8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: December 1, 2000
Last modified: June 11, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names