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Protein

Anthranilate synthase component 1

Gene

trpE

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia (By similarity).By similarity

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Enzyme regulationi

Feedback inhibited by tryptophan.By similarity

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Anthranilate synthase component 1 (trpE), Anthranilate synthase component 1 (trpE), Anthranilate synthase component 2 (trpG)
  2. Anthranilate phosphoribosyltransferase (trpD)
  3. Tryptophan biosynthesis protein TrpCF (trpC)
  4. Tryptophan biosynthesis protein TrpCF (trpC)
  5. Tryptophan synthase alpha chain (trpA), Tryptophan synthase beta chain (trpB)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401TryptophanBy similarity
Metal bindingi362 – 3621MagnesiumBy similarity
Binding sitei450 – 4501ChorismateBy similarity
Binding sitei470 – 4701ChorismateBy similarity
Binding sitei486 – 4861Chorismate; via amide nitrogenBy similarity
Metal bindingi499 – 4991MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-616-MONOMER.
UniPathwayiUPA00035; UER00040.

Names & Taxonomyi

Protein namesi
Recommended name:
Anthranilate synthase component 1 (EC:4.1.3.27)
Short name:
AS
Short name:
ASI
Gene namesi
Name:trpE
Ordered Locus Names:BUpT01
Encoded oniPlasmid pTrp (pBAp)0 Publication
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
Proteomesi
  • UP000001806 Componenti: Plasmid pTrp

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 521521Anthranilate synthase component 1PRO_0000154080Add
BLAST

Proteomic databases

PRIDEiQ44695.

Interactioni

Subunit structurei

Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ44695.
SMRiQ44695. Positions 9-516.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni292 – 2943Tryptophan bindingBy similarity
Regioni329 – 3302Chorismate bindingBy similarity
Regioni484 – 4863Chorismate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000025144.
KOiK01657.
OMAiMQDQDFT.
OrthoDBiEOG6D5G6B.

Family and domain databases

Gene3Di3.60.120.10. 1 hit.
InterProiIPR005801. ADC_synthase.
IPR019999. Anth_synth_I-like.
IPR006805. Anth_synth_I_N.
IPR005257. Anth_synth_I_TrpE.
IPR015890. Chorismate_C.
[Graphical view]
PfamiPF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
[Graphical view]
PIRSFiPIRSF001373. TrpE. 1 hit.
PRINTSiPR00095. ANTSNTHASEI.
SUPFAMiSSF56322. SSF56322. 1 hit.
TIGRFAMsiTIGR00565. trpE_proteo. 1 hit.

Sequencei

Sequence statusi: Complete.

Q44695-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLIEKRRKL IQKKANYHSD PTTVFNHLCG SRPATLLLET AEVNKKNNLE
60 70 80 90 100
SIMIVDSAIR VSAVKNSVKI TALSENGAEI LSILKENPHK KIKFFEKNKS
110 120 130 140 150
INLIFPSLDN NLDEDKKIFS LSVFDSFRFI MKSVNNTKRT SKAMFFGGLF
160 170 180 190 200
SYDLISNFES LPNVKKKQKC PDFCFYLAET LLVVDHQKKT CLIQSSLFGR
210 220 230 240 250
NVDEKNRIKK RTEEIEKKLE EKLTSIPKNK TTVPVQLTSN ISDFQYSSTI
260 270 280 290 300
KKLQKLIQKG EIFQVVPSRK FFLPCDNSLS AYQELKKSNP SPYMFFMQDE
310 320 330 340 350
DFILFGASPE SSLKYDEKNR QIELYPIAGT RPRGRKKDGT LDLDLDSRIE
360 370 380 390 400
LEMRTNHKEL AEHLMLVDLA RNDLARICEP GSRYVSDLVK VDKYSHVMHL
410 420 430 440 450
VSKVVGQLKY GLDALHAYSS CMNMGTLTGA PKVRAMQLIA EYEGEGRGSY
460 470 480 490 500
GGAIGYFTDL GNLDTCITIR SAYVESGVAT IQAGAGVVFN SIPEDEVKES
510 520
LNKAQAVINA IKKAHFTMGS S
Length:521
Mass (Da):58,793
Last modified:December 1, 2000 - v3
Checksum:i1F09602A47F0E7C5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 1010MFLIEKRRKL → MQKSPYEIEI in AAD09346 (PubMed:8642610).Curated
Sequence conflicti276 – 2761D → N in AAD09346 (PubMed:8642610).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43555 Genomic DNA. Translation: AAD09346.1.
AP001070 Genomic DNA. Translation: BAA95417.1.
RefSeqiNP_057962.1. NC_002252.1.
WP_010892289.1. NC_002252.1.

Genome annotation databases

EnsemblBacteriaiBAA95417; BAA95417; BAA95417.
GeneIDi1109444.
KEGGibuc:BUpT01.
PATRICi21243478. VBIBucAph127364_0001.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43555 Genomic DNA. Translation: AAD09346.1.
AP001070 Genomic DNA. Translation: BAA95417.1.
RefSeqiNP_057962.1. NC_002252.1.
WP_010892289.1. NC_002252.1.

3D structure databases

ProteinModelPortaliQ44695.
SMRiQ44695. Positions 9-516.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ44695.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA95417; BAA95417; BAA95417.
GeneIDi1109444.
KEGGibuc:BUpT01.
PATRICi21243478. VBIBucAph127364_0001.

Phylogenomic databases

HOGENOMiHOG000025144.
KOiK01657.
OMAiMQDQDFT.
OrthoDBiEOG6D5G6B.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.
BioCyciBAPH107806:GBZJ-616-MONOMER.

Family and domain databases

Gene3Di3.60.120.10. 1 hit.
InterProiIPR005801. ADC_synthase.
IPR019999. Anth_synth_I-like.
IPR006805. Anth_synth_I_N.
IPR005257. Anth_synth_I_TrpE.
IPR015890. Chorismate_C.
[Graphical view]
PfamiPF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
[Graphical view]
PIRSFiPIRSF001373. TrpE. 1 hit.
PRINTSiPR00095. ANTSNTHASEI.
SUPFAMiSSF56322. SSF56322. 1 hit.
TIGRFAMsiTIGR00565. trpE_proteo. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The tryptophan biosynthetic pathway of aphid endosymbionts (Buchnera): genetics and evolution of plasmid-associated anthranilate synthase (trpEG) within the aphididae."
    Rouhbakhsh D., Lai C.-Y., von Dohlen C.D., Clark M.A., Baumann L., Baumann P., Moran N.A., Voegtlin D.J.
    J. Mol. Evol. 42:414-421(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiTRPE_BUCAI
AccessioniPrimary (citable) accession number: Q44695
Secondary accession number(s): Q9KGQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: December 1, 2000
Last modified: January 20, 2016
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.