Reviewed, UniProtKB/Swiss-Prot Q44695 (TRPE_BUCAI)

Names and origin

Protein names

Recommended name:
    Anthranilate synthase component 1
    EC=4.1.3.27
Alternative name(s):
    Anthranilate synthase component I

Gene names

Name:	trpE
Ordered Locus Names:	BUpT01

Encoded on

Plasmid pTrp (pBAp)

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	521 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.
Subunit structure	Tetramer of two components I and two components II By similarity.
Miscellaneous	Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.
Sequence similarities	Belongs to the anthranilate synthase component I family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Lyase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	tryptophan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	anthranilate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 521	521	Anthranilate synthase component 1		PRO_0000154080
Experimental info
	Sequence conflict	1 – 10	10	MFLIEKRRKL → MQKSPYEIEI Ref.1
	Sequence conflict	276	1	D → N in AAD09346. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q44695-1 [UniParc]. Last modified December 1, 2000. Version 3. Checksum: 1F09602A47F0E7C5	FASTA	521	58,793
	10 20 30 40 50 60 MFLIEKRRKL IQKKANYHSD PTTVFNHLCG SRPATLLLET AEVNKKNNLE SIMIVDSAIR 70 80 90 100 110 120 VSAVKNSVKI TALSENGAEI LSILKENPHK KIKFFEKNKS INLIFPSLDN NLDEDKKIFS 130 140 150 160 170 180 LSVFDSFRFI MKSVNNTKRT SKAMFFGGLF SYDLISNFES LPNVKKKQKC PDFCFYLAET 190 200 210 220 230 240 LLVVDHQKKT CLIQSSLFGR NVDEKNRIKK RTEEIEKKLE EKLTSIPKNK TTVPVQLTSN 250 260 270 280 290 300 ISDFQYSSTI KKLQKLIQKG EIFQVVPSRK FFLPCDNSLS AYQELKKSNP SPYMFFMQDE 310 320 330 340 350 360 DFILFGASPE SSLKYDEKNR QIELYPIAGT RPRGRKKDGT LDLDLDSRIE LEMRTNHKEL 370 380 390 400 410 420 AEHLMLVDLA RNDLARICEP GSRYVSDLVK VDKYSHVMHL VSKVVGQLKY GLDALHAYSS 430 440 450 460 470 480 CMNMGTLTGA PKVRAMQLIA EYEGEGRGSY GGAIGYFTDL GNLDTCITIR SAYVESGVAT 490 500 510 520 IQAGAGVVFN SIPEDEVKES LNKAQAVINA IKKAHFTMGS S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The tryptophan biosynthetic pathway of aphid endosymbionts (Buchnera): genetics and evolution of plasmid-associated anthranilate synthase (trpEG) within the aphididae." Rouhbakhsh D., Lai C.-Y., von Dohlen C.D., Clark M.A., Baumann L., Baumann P., Moran N.A., Voegtlin D.J. J. Mol. Evol. 42:414-421(1996) [PubMed: 8642610] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L43555 Genomic DNA. Translation: AAD09346.1. AP001070 Genomic DNA. Translation: BAA95417.1.
RefSeq	NP_057962.1.
3D structure databases
HSSP	HSSP built from PDB template 1I1Q based on UniProtKB P00898.
ModBase	Search...
Genome annotation databases
GeneID	1109444.
GenomeReviews	Gene locus BUpT01 in contig AP001070_GR.
KEGG	buc:BUpT01.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q44695.
OMA	Q44695. HKELAEH.
Enzyme and pathway databases
BioCyc	BSP107806:BUPT01-MON.
Family and domain databases
InterPro	IPR005801. ADC_synthase. IPR006805. Anth_synth_I_N. IPR019999. Anthranilate_synth_I_C. IPR015890. Chorismate-bd_C. IPR005257. TrpE_synth. [Graphical view]
Gene3D	G3DSA:3.60.120.10. TRPE_1_chor_bd. 1 hit.
PANTHER	PTHR11236. TRPE_1_chor_bd. 1 hit.
Pfam	PF04715. Anth_synt_I_N. 1 hit. PF00425. Chorismate_bind. 1 hit. [Graphical view]
PRINTS	PR00095. ANTSNTHASEI.
ProDom	PD000779. Anth_synth_chor. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00565. trpE_proteo. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRPE_BUCAI

Accession

Primary (citable) accession number: Q44695
Secondary accession number(s): Q9KGQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	December 1, 2000
Last modified:	June 16, 2009
This is version 67 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)