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Protein

Anthranilate synthase component 1

Gene

trpE

Organism
Buchnera aphidicola subsp. Rhopalosiphum padi
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia (By similarity).By similarity

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Enzyme regulationi

Feedback inhibited by tryptophan.By similarity

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Anthranilate synthase component 1 (trpE)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Tryptophan synthase beta chain (trpB)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401TryptophanBy similarity
Metal bindingi360 – 3601MagnesiumBy similarity
Binding sitei448 – 4481ChorismateBy similarity
Binding sitei468 – 4681ChorismateBy similarity
Binding sitei484 – 4841Chorismate; via amide nitrogenBy similarity
Metal bindingi497 – 4971MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.

Names & Taxonomyi

Protein namesi
Recommended name:
Anthranilate synthase component 1 (EC:4.1.3.27)
Short name:
AS
Short name:
ASI
Gene namesi
Name:trpE
Encoded oniPlasmid pBRp0 Publication
OrganismiBuchnera aphidicola subsp. Rhopalosiphum padi
Taxonomic identifieri98793 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 514514Anthranilate synthase component 1PRO_0000154086Add
BLAST

Interactioni

Subunit structurei

Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ44691.
SMRiQ44691. Positions 7-513.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni290 – 2923Tryptophan bindingBy similarity
Regioni327 – 3282Chorismate bindingBy similarity
Regioni482 – 4843Chorismate bindingBy similarity

Sequence similaritiesi

Family and domain databases

Gene3Di3.60.120.10. 1 hit.
InterProiIPR005801. ADC_synthase.
IPR019999. Anth_synth_I-like.
IPR006805. Anth_synth_I_N.
IPR005257. Anth_synth_I_TrpE.
IPR015890. Chorismate_C.
[Graphical view]
PfamiPF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
[Graphical view]
PIRSFiPIRSF001373. TrpE. 1 hit.
PRINTSiPR00095. ANTSNTHASEI.
SUPFAMiSSF56322. SSF56322. 1 hit.
TIGRFAMsiTIGR00565. trpE_proteo. 1 hit.

Sequencei

Sequence statusi: Complete.

Q44691-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKSAYPIEI IQKQAPYHPD PTMIFNHLCE SRSETLLLET ADNKKKRSRK
60 70 80 90 100
IMIIDSAMRI SSEHNAVKLT PLSINGMEIL LVLKKIISKK IEIYRRKKNT
110 120 130 140 150
ILIFPNIEKD LDEDKKLFSL SVFDAFRLII KTFENREKKS KAMFFGGLFS
160 170 180 190 200
YDLISVFETL PKLKGNQKCS NFCFYLAETL LVLDHQQKTC LIQSSLFTKN
210 220 230 240 250
SNEKKRIEER SVEIKQKLNQ KLNSIPKIKI KDINLTSNMN NFEYGSIIKK
260 270 280 290 300
LQKLIQKGEI FQVVPSRKFY LPCPNPLSAY QKLKKSNPSP YMFFMQDQDF
310 320 330 340 350
TLFGASPESS LKYDEKTRKI ELYPIAGTRP RGRTKDGNLD LDLDSRIELE
360 370 380 390 400
MRTNHKELAE HLMLVDLARN DLARICKPGS RYVSDLVRVD RYSHVMHLVS
410 420 430 440 450
RVIGELREGL DALHAYASCM NMGTLTGAPK VCAMQLIAEY EKEKRGSYGG
460 470 480 490 500
AIGYFTDLGN LDTCITIRSA YVEKRYCYNQ AGAGIVYNSI PEDEVNESLN
510
KAQAVINAIK NAHY
Length:514
Mass (Da):58,985
Last modified:November 1, 1996 - v1
Checksum:i3B35BCF38DB434B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43551 Genomic DNA. Translation: AAB05986.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43551 Genomic DNA. Translation: AAB05986.1.

3D structure databases

ProteinModelPortaliQ44691.
SMRiQ44691. Positions 7-513.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.

Family and domain databases

Gene3Di3.60.120.10. 1 hit.
InterProiIPR005801. ADC_synthase.
IPR019999. Anth_synth_I-like.
IPR006805. Anth_synth_I_N.
IPR005257. Anth_synth_I_TrpE.
IPR015890. Chorismate_C.
[Graphical view]
PfamiPF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
[Graphical view]
PIRSFiPIRSF001373. TrpE. 1 hit.
PRINTSiPR00095. ANTSNTHASEI.
SUPFAMiSSF56322. SSF56322. 1 hit.
TIGRFAMsiTIGR00565. trpE_proteo. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The tryptophan biosynthetic pathway of aphid endosymbionts (Buchnera): genetics and evolution of plasmid-associated anthranilate synthase (trpEG) within the aphididae."
    Rouhbakhsh D., Lai C.-Y., von Dohlen C.D., Clark M.A., Baumann L., Baumann P., Moran N.A., Voegtlin D.J.
    J. Mol. Evol. 42:414-421(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiTRPE_BUCRP
AccessioniPrimary (citable) accession number: Q44691
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: January 20, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.