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Protein

Arginine--tRNA ligase

Gene

argS

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).

GO - Molecular functioni

  1. arginine-tRNA ligase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. arginyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-242-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Arginine--tRNA ligase (EC:6.1.1.19)
Alternative name(s):
Arginyl-tRNA synthetase
Short name:
ArgRS
Gene namesi
Name:argS
Ordered Locus Names:BU242
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 574574Arginine--tRNA ligasePRO_0000151539Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ44683.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi121 – 13111"HIGH" regionAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0018.
HOGENOMiHOG000247212.
KOiK01887.
OMAiVGFGTMM.
OrthoDBiEOG6JB13C.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.30.1360.70. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00123. Arg_tRNA_synth.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR001278. Arg-tRNA-ligase.
IPR005148. Arg-tRNA-synth_N.
IPR008909. DALR_anticod-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERiPTHR11956. PTHR11956. 1 hit.
PfamiPF03485. Arg_tRNA_synt_N. 1 hit.
PF05746. DALR_1. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
[Graphical view]
PRINTSiPR01038. TRNASYNTHARG.
SMARTiSM01016. Arg_tRNA_synt_N. 1 hit.
SM00836. DALR_1. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF55190. SSF55190. 1 hit.
TIGRFAMsiTIGR00456. argS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q44683-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLKNTIKED IQDALIKIAI INCDPVITLN KKTKIGHYQL NNLIKIANIS
60 70 80 90 100
NLKPFELANR IILNIKKKYM YKEITFSQPG FINFLINPYW ISEQLEKIFL
110 120 130 140 150
SPRLGINHVN AQNIVIDYSS PNIAKEMHIG HLRSTIIGDV MARILDFLGH
160 170 180 190 200
NVIRANHIGD WGTQFGMLIA YLEVKKLVNS PLSLMKLEEY YCKAKKKYDI
210 220 230 240 250
DQLFAEKSRE YVVKLQNGDQ YCYSIWKKLV SITMLENCKI YKRLHVTLKK
260 270 280 290 300
KHTMGESIYN KMLPNIIEDL KNKKIAIEKN GSTIVFLKEF KNRLGEPMGV
310 320 330 340 350
VIQKKDKGFL YSTTDIACLK YRYQVLHADR IIYYTDSRQH QHLLQAWTIA
360 370 380 390 400
KKALYISQNL LLEHHIFGMM LSKDKRPFKT RDGDTIKLSA LLDEATERAM
410 420 430 440 450
RLIKNKQPNL SKKKLNQLSN IIGVGAVKYA DLSKNRNTNY IFDWNEMLSF
460 470 480 490 500
EGNTAPYIQY AYTRIVSILK KSNMPIKKLK EKIFLTKESE INLAIKILEF
510 520 530 540 550
EEIILLISQK GTPHILCKYL YHLATSFSHF YENCSILFPK KIKTCKSRLK
560 570
LSILTAKTLK KGLNMLGIRV VKKM
Length:574
Mass (Da):66,686
Last modified:December 1, 2000 - v2
Checksum:i7916BD29FCAB02C1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91E → K in AAA72384 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12957.1.
L18933 Genomic DNA. Translation: AAA72384.1.
RefSeqiNP_240071.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12957; BAB12957; BAB12957.
GeneIDi1109685.
KEGGibuc:BU242.
PATRICi21244006. VBIBucAph127364_0255.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12957.1.
L18933 Genomic DNA. Translation: AAA72384.1.
RefSeqiNP_240071.1. NC_002528.1.

3D structure databases

ProteinModelPortaliQ44683.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB12957; BAB12957; BAB12957.
GeneIDi1109685.
KEGGibuc:BU242.
PATRICi21244006. VBIBucAph127364_0255.

Phylogenomic databases

eggNOGiCOG0018.
HOGENOMiHOG000247212.
KOiK01887.
OMAiVGFGTMM.
OrthoDBiEOG6JB13C.

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-242-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.30.1360.70. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00123. Arg_tRNA_synth.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR001278. Arg-tRNA-ligase.
IPR005148. Arg-tRNA-synth_N.
IPR008909. DALR_anticod-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERiPTHR11956. PTHR11956. 1 hit.
PfamiPF03485. Arg_tRNA_synt_N. 1 hit.
PF05746. DALR_1. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
[Graphical view]
PRINTSiPR01038. TRNASYNTHARG.
SMARTiSM01016. Arg_tRNA_synt_N. 1 hit.
SM00836. DALR_1. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF55190. SSF55190. 1 hit.
TIGRFAMsiTIGR00456. argS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.
  2. "Partial characterization of the ribosomal RNA operons of the pea aphid endosymbionts: evolution and physiological implications."
    Unterman B.M., Baumann P.
    (In) Campbell R.K., Eikenbary R.D. (eds.); Aphid-plant genotype interactions, pp.329-350, Elsevier, Amsterdam (1990)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-158.

Entry informationi

Entry nameiSYR_BUCAI
AccessioniPrimary (citable) accession number: Q44683
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: December 1, 2000
Last modified: January 7, 2015
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.