Reviewed, UniProtKB/Swiss-Prot Q44683 (SYR_BUCAI)

Names and origin

Protein names

Recommended name:
    Arginyl-tRNA synthetase
    EC=6.1.1.19
Alternative name(s):
    Arginine--tRNA ligase
      Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	BU242

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	574 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP MF_00123
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00123
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 574	574	Arginyl-tRNA synthetase HAMAP MF_00123		PRO_0000151539
Regions
	Motif	121 – 131	11	"HIGH" region HAMAP MF_00123
Experimental info
	Sequence conflict	9	1	E → K in AAA72384. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q44683-1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 7916BD29FCAB02C1	FASTA	574	66,686
	10 20 30 40 50 60 MNLKNTIKED IQDALIKIAI INCDPVITLN KKTKIGHYQL NNLIKIANIS NLKPFELANR 70 80 90 100 110 120 IILNIKKKYM YKEITFSQPG FINFLINPYW ISEQLEKIFL SPRLGINHVN AQNIVIDYSS 130 140 150 160 170 180 PNIAKEMHIG HLRSTIIGDV MARILDFLGH NVIRANHIGD WGTQFGMLIA YLEVKKLVNS 190 200 210 220 230 240 PLSLMKLEEY YCKAKKKYDI DQLFAEKSRE YVVKLQNGDQ YCYSIWKKLV SITMLENCKI 250 260 270 280 290 300 YKRLHVTLKK KHTMGESIYN KMLPNIIEDL KNKKIAIEKN GSTIVFLKEF KNRLGEPMGV 310 320 330 340 350 360 VIQKKDKGFL YSTTDIACLK YRYQVLHADR IIYYTDSRQH QHLLQAWTIA KKALYISQNL 370 380 390 400 410 420 LLEHHIFGMM LSKDKRPFKT RDGDTIKLSA LLDEATERAM RLIKNKQPNL SKKKLNQLSN 430 440 450 460 470 480 IIGVGAVKYA DLSKNRNTNY IFDWNEMLSF EGNTAPYIQY AYTRIVSILK KSNMPIKKLK 490 500 510 520 530 540 EKIFLTKESE INLAIKILEF EEIILLISQK GTPHILCKYL YHLATSFSHF YENCSILFPK 550 560 570 KIKTCKSRLK LSILTAKTLK KGLNMLGIRV VKKM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.
[2]	"Partial characterization of the ribosomal RNA operons of the pea aphid endosymbionts: evolution and physiological implications." Unterman B.M., Baumann P. (In) Campbell R.K., Eikenbary R.D. (eds.); Aphid-plant genotype interactions, pp.329-350, Elsevier, Amsterdam (1990) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-158.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB12957.1. L18933 Genomic DNA. Translation: AAA72384.1.
RefSeq	NP_240071.1.
3D structure databases
HSSP	HSSP built from PDB template 1IQ0 based on UniProtKB Q93RP5.
ModBase	Search...
Genome annotation databases
GeneID	1109685.
GenomeReviews	Gene locus BU242 in contig BA000003_GR.
KEGG	buc:BU242.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q44683.
OMA	Q44683. YNDDLQP.
Enzyme and pathway databases
BioCyc	BSP107806:BU242-MON.
Family and domain databases
HAMAP	MF_00123. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-synth_Ic. IPR015945. Arg-tRNA-synth_Ic_core. IPR005148. Arg-tRNA-synth_Ic_N. IPR008909. DALR_anticod_bd. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11956. Arg_tRNA-synt_1c. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
TIGRFAMs	TIGR00456. argS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_BUCAI

Accession

Primary (citable) accession number: Q44683

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	December 1, 2000
Last modified:	June 16, 2009
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)