Q44683 (SYR_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine--tRNA ligase
EC=6.1.1.19

Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	BU242

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Acyrthosiphon pisum)

Protein attributes

Sequence length	574 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP-Rule MF_00123
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 574	574	Arginine--tRNA ligase HAMAP-Rule MF_00123		PRO_0000151539
Regions
	Motif	121 – 131	11	"HIGH" region HAMAP-Rule MF_00123
Experimental info
	Sequence conflict	9	1	E → K in AAA72384. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q44683 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 7916BD29FCAB02C1	FASTA	574	66,686
	10 20 30 40 50 60 MNLKNTIKED IQDALIKIAI INCDPVITLN KKTKIGHYQL NNLIKIANIS NLKPFELANR 70 80 90 100 110 120 IILNIKKKYM YKEITFSQPG FINFLINPYW ISEQLEKIFL SPRLGINHVN AQNIVIDYSS 130 140 150 160 170 180 PNIAKEMHIG HLRSTIIGDV MARILDFLGH NVIRANHIGD WGTQFGMLIA YLEVKKLVNS 190 200 210 220 230 240 PLSLMKLEEY YCKAKKKYDI DQLFAEKSRE YVVKLQNGDQ YCYSIWKKLV SITMLENCKI 250 260 270 280 290 300 YKRLHVTLKK KHTMGESIYN KMLPNIIEDL KNKKIAIEKN GSTIVFLKEF KNRLGEPMGV 310 320 330 340 350 360 VIQKKDKGFL YSTTDIACLK YRYQVLHADR IIYYTDSRQH QHLLQAWTIA KKALYISQNL 370 380 390 400 410 420 LLEHHIFGMM LSKDKRPFKT RDGDTIKLSA LLDEATERAM RLIKNKQPNL SKKKLNQLSN 430 440 450 460 470 480 IIGVGAVKYA DLSKNRNTNY IFDWNEMLSF EGNTAPYIQY AYTRIVSILK KSNMPIKKLK 490 500 510 520 530 540 EKIFLTKESE INLAIKILEF EEIILLISQK GTPHILCKYL YHLATSFSHF YENCSILFPK 550 560 570 KIKTCKSRLK LSILTAKTLK KGLNMLGIRV VKKM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.
[2]	"Partial characterization of the ribosomal RNA operons of the pea aphid endosymbionts: evolution and physiological implications." Unterman B.M., Baumann P. (In) Campbell R.K., Eikenbary R.D. (eds.); Aphid-plant genotype interactions, pp.329-350, Elsevier, Amsterdam (1990) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-158.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12957.1. L18933 Genomic DNA. Translation: AAA72384.1.
RefSeq	NP_240071.1. NC_002528.1.
3D structure databases
ProteinModelPortal	Q44683.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB12957; BAB12957; BAB12957.
GeneID	1109685.
KEGG	buc:BU242.
PATRIC	21244006. VBIBucAph127364_0255.
Phylogenomic databases
eggNOG	COG0018.
HOGENOM	HOG000247212.
KO	K01887.
OMA	DRGESFY.
ProtClustDB	PRK01611.
Family and domain databases
Gene3D	3.30.1360.70. 1 hit. 3.40.50.620. 1 hit.
HAMAP	MF_00123. Arg_tRNA_synth.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-ligase_Ia. IPR015945. Arg-tRNA-synth_Ia_core. IPR005148. Arg-tRNA-synth_N. IPR008909. DALR_anticod-bd. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
PANTHER	PTHR11956. PTHR11956. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
SMART	SM01016. Arg_tRNA_synt_N. 1 hit. SM00836. DALR_1. 1 hit. [Graphical view]
SUPFAM	SSF55190. Arg-tRNA-synth_Ic_N. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00456. argS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_BUCAI

Accession

Primary (citable) accession number: Q44683

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program