Q44681 (RISB_BACAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6,7-dimethyl-8-ribityllumazine synthase
Short name=DMRL synthase
Short name=Lumazine synthase
EC=2.5.1.9

Alternative name(s):
Riboflavin synthase beta chain

Gene names

Name:

ribH

Organism

Bacillus amyloliquefaciens (Bacillus velezensis)

Taxonomic identifier

1390 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	154 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate yielding 6,7-dimethyl-8-lumazine By similarity. HAMAP MF_00178
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine. HAMAP MF_00178
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2. HAMAP MF_00178
Sequence similarities	Belongs to the DMRL synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Molecular function	Transferase
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	riboflavin synthase complex Inferred from electronic annotation. Source: InterPro
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 154	154	6,7-dimethyl-8-ribityllumazine synthase HAMAP MF_00178		PRO_0000134711

Sequences

	Sequence		Length	Mass (Da)
	Q44681 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: DA385F242E0399C8	FASTA	154	16,219
	10 20 30 40 50 60 MNIIQGNLVG TGLKIGIVVG RFNEFITSKL LSGAEDTLIR HGVESNDIDV AWVPGAFEIP 70 80 90 100 110 120 FAAKKMAETK KYDAVITLGT VIRGATTHYD YVCNEAAKGI AQAGTATGVP VIFGIVTTET 130 140 150 IEQAIERAGT KAGNKGADCA VSAIEMANLN RSFE

References

[1]	"Riboflavin biosynthetic genes in Bacillus amyloliquefaciens: primary structure, organization and regulation of activity." Gusarov I.I., Kreneva R.A., Podcharniaev D.A., Iomantas I.U.V., Abalakina E.G., Stoinova N.V., Perumov D.A., Kozlov I.U.I. Mol. Biol. (Mosk.) 31:446-453(1997) [PubMed: 9297088] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A 50.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X95955 Genomic DNA. Translation: CAA65192.1.
PIR	T50544.
3D structure databases
ProteinModelPortal	Q44681.
SMR	Q44681. Positions 1-154.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_00178. Lumazine_synth. [Tree]
InterPro	IPR002180. DMRL_synthase. [Graphical view]
Gene3D	G3DSA:3.40.50.960. DMRL_synthase. 1 hit.
PANTHER	PTHR21058. DMRL_synthase. 1 hit.
Pfam	PF00885. DMRL_synthase. 1 hit. [Graphical view]
SUPFAM	SSF52121. DMRL_synthase. 1 hit.
TIGRFAMs	TIGR00114. Lumazine-synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RISB_BACAM

Accession

Primary (citable) accession number: Q44681

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 31, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program