Q44678 (PURK_CORAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N5-carboxyaminoimidazole ribonucleotide synthase
Short name=N5-CAIR synthase
EC=6.3.4.18

Alternative name(s):
5-(carboxyamino)imidazole ribonucleotide synthetase

Gene names

Name:

purK

Organism

Corynebacterium ammoniagenes (Brevibacterium ammoniagenes)

Taxonomic identifier

1697 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO3- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) By similarity.
Catalytic activity	ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3- = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 1/2.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the purK/purT family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	5-(carboxyamino)imidazole ribonucleotide synthase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: InterPro phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 413	413	N5-carboxyaminoimidazole ribonucleotide synthase		PRO_0000074996
Regions
	Domain	126 – 319	194	ATP-grasp
	Nucleotide binding	199 – 202	4	ATP By similarity
	Nucleotide binding	289 – 290	2	ATP By similarity
Sites
	Binding site	122	1	ATP By similarity
	Binding site	162	1	ATP By similarity
	Binding site	207	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q44678 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F738B230DF5D119A	FASTA	413	44,111
	10 20 30 40 50 60 MKRVSEQAGN PDGNPQAHVP GMPVIAVIGD GQLARMMQTA AIELGQSLRL LAGARDASAA 70 80 90 100 110 120 QVCADVVLGD YTNYDDLLKA VDGATAVTFD HEHVPNEHLT ALIDAGYNDQ PQPAALINAQ 130 140 150 160 170 180 DKLVMRERLA ELGAPVPRFA PIESAQDAYD FWTLTSGQVC LKARRGGYDG KGVWFPNNES 190 200 210 220 230 240 ELTALVSDLS RRGVALMAEE KVGWSRELSV LVARTPSGEV ATWPLTESVQ RNGVCAEAVA 250 260 270 280 290 300 PAPGVDPQLQ QRAETLGEKI ATELGVTGVL AVELFAFANE SGAEDIAVNE LAMRPHNTGH 310 320 330 340 350 360 WTLVGSVTSQ FEQHLRAVMD EPLGDTSTLA PVTVMANVLG ADEDQRCQWA SVPRSGAPVP 370 380 390 400 410 ATKVHLYGKG IAQGRKIGHV NLTGEDEEAT RRDARLAADF LVNAAWSDNW SAK

References

[1]	"Genomic organization of purK and purE in Brevibacterium ammoniagenes ATCC 6872: purE locus provides a clue for genomic evolution." Chung S.O., Lee J.H., Lee S.Y., Lee D.S. FEMS Microbiol. Lett. 137:265-268(1996) [PubMed: 8998996] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 6872 / DSM 20305 / KCTC 1019 / NCTC 2399.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X91189 Genomic DNA. Translation: CAA62598.1.
3D structure databases
ProteinModelPortal	Q44678.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR005875. AIR_COase_ATPase-su. IPR011761. ATP-grasp. IPR003135. ATP-grasp_carboxylate-amine. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF02222. ATP-grasp. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR01161. PurK. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURK_CORAM

Accession

Primary (citable) accession number: Q44678

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	September 21, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program