Reviewed, UniProtKB/Swiss-Prot Q44674 (BLAC_BACAM)

Names and origin

Protein names

Recommended name:
    Beta-lactamase
    EC=3.5.2.6
Alternative name(s):
    Penicillinase

Gene names

Name:

penP

Organism

Bacillus amyloliquefaciens

Taxonomic identifier

1390 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein is a beta-lactamase with a substrate specificity for penicillins.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34	By similarity
	Chain	35 – 306	272	Beta-lactamase		PRO_0000016969
Regions
	Region	251 – 253	3	Substrate binding By similarity
Sites
	Active site	89	1	Acyl-ester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q44674-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: BEC57A283995E415	FASTA	306	33,409
	10 20 30 40 50 60 MNVKRKATLK FGICIGLLCV SFTGFNSLFG STHAEAKSIE NTKMTSCITN QKFVQLEKKF 70 80 90 100 110 120 DARLGVYAID TGSNKTIAYR PNERFAYAST YKVLAAAAVL KQKPIEKLND VIRYTKEDLV 130 140 150 160 170 180 TYSPITEKHL DTGMSLKEIS EAAIRYSDNT AGNILLQQLG GPKGFEKSLK QIGDHVTKAD 190 200 210 220 230 240 RFETDLNSAI PGDIRDTSTA KALATDLKAF TLGNTLTTDK RTILTDWMRG NATGDELIRA 250 260 270 280 290 300 GAPAGWEVGD KSGAGSYGTR NDIAIVWPPD RAPIVLAILT KRFTKDAEYD NALIAEAAKV ALDDLK

References

[1]	"Identification of penicillinase-encoding genes of Bacillus amyloliquefaciens and Bacillus subtilis." van Dijl J.M., de Jong A., Nauta A., Venema G., Bron S. Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z35653 Genomic DNA. Translation: CAA84712.1.
PIR	S47330.
3D structure databases
HSSP	HSSP built from PDB template 1I2W based on UniProtKB P00808.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.5.2.6. 1130.
Family and domain databases
InterPro	IPR001466. Beta_lactamase-related. IPR000871. Beta_lactamase_A/D. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLAC_BACAM

Accession

Primary (citable) accession number: Q44674

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)