Reviewed, UniProtKB/Swiss-Prot Q44607 (AROE_BUCAI)

Names and origin

Protein names

Recommended name:
    Shikimate dehydrogenase
    EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	BU493

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and PEP: step 4/7. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.
Sequence caution	The sequence AAA79125.1 differs from that shown. Reason: Frameshift at position 265.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro shikimate 5-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	Shikimate dehydrogenase HAMAP MF_00222		PRO_0000135995
Regions
	Nucleotide binding	131 – 135	5	NADP By similarity
Sites
	Active site	70	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	Q44607-1 [UniParc]. Last modified December 1, 2000. Version 3. Checksum: 4EAA9019E77213F9	FASTA	273	31,002
	10 20 30 40 50 60 MFKCKNFNYA VFGNPINHSK SPEIHSLFSK QTGISHFYKS CNVPLNSLYA VLQDFFKKDG 70 80 90 100 110 120 RGANITAPFK QEAYFFCNKL TKRAEVAQSV NTLKKIDNCN ILGDNTDGIG LLSDLIRLNF 130 140 150 160 170 180 IKKNYSILII GAGGAARGVL FPLLSYGCSI CIFNRTVLNA EKLVLQFHKY GNINVFNTNS 190 200 210 220 230 240 LHVKSFDLII NATSHFIQDK DNFIPFSCVS SKTCFYDMNY QTDNTFFFDW SRKTGSNFFS 250 260 270 NGIGMLVFQA AHSFFLWHNV LPEIDYIIDL LNK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.
[2]	"Characterization of a putative 23S-5S rRNA operon of Buchnera aphidicola (endosymbiont of aphids) unlinked to the 16S rRNA-encoding gene." Rouhbakhsh D., Baumann P. Gene 155:107-112(1995) [PubMed: 7535281] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 253-273.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB13186.1. U10496 Genomic DNA. Translation: AAA79125.1. Frameshift.
PIR	I40062.
RefSeq	NP_240300.1.
3D structure databases
HSSP	HSSP built from PDB template 1NYT based on UniProtKB P15770.
ModBase	Search...
Genome annotation databases
GeneID	1109836.
GenomeReviews	Gene locus BU493 in contig BA000003_GR.
KEGG	buc:BU493.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q44607.
OMA	Q44607. YDMMYGR.
Enzyme and pathway databases
BioCyc	BSP107806:BU493-MON.
Family and domain databases
HAMAP	MF_00222. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR011342. Quinate/shikimate_5-DH. IPR013708. Shikimate_DH-bd_N. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00507. aroE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROE_BUCAI

Accession

Primary (citable) accession number: Q44607

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	December 1, 2000
Last modified:	June 16, 2009
This is version 61 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)