Q44603 (TRPC_BUCSC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan biosynthesis protein TrpCF

Including the following 2 domains:

1. Indole-3-glycerol phosphate synthase
   Short name=IGPS
   EC=4.1.1.48
2. N-(5'-phospho-ribosyl)anthranilate isomerase
   Short name=PRAI
   EC=5.3.1.24

Gene names

Name:	trpC
Synonyms:	trpC/F

Organism

Buchnera aphidicola subsp. Schlechtendalia chinensis

Taxonomic identifier

118110 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes two sequential steps of tryptophan biosynthetic pathway. The first reaction is catalyzed by the isomerase, coded by the TrpF domain; the second reaction is catalyzed by the synthase, coded by the TrpC domain By similarity. HAMAP-Rule MF_00134_B
Catalytic activity	N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate. HAMAP-Rule MF_00134_B 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O. HAMAP-Rule MF_00134_B
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5. HAMAP-Rule MF_00134_B Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.
Sequence similarities	In the N-terminal section; belongs to the TrpC family. In the C-terminal section; belongs to the TrpF family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Decarboxylase Isomerase Lyase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological_process	tryptophan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	indole-3-glycerol-phosphate synthase activity Inferred from electronic annotation. Source: EC phosphoribosylanthranilate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	Tryptophan biosynthesis protein TrpCF HAMAP-Rule MF_00134_B		PRO_0000154275
Regions
	Region	1 – 258	258	Indole-3-glycerol phosphate synthase HAMAP-Rule MF_00134_B
	Region	259 – 461	203	N-(5'-phosphoribosyl)anthranilate isomerase HAMAP-Rule MF_00134_B

Sequences

	Sequence		Length	Mass (Da)
	Q44603 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F202C81D6419D68D	FASTA	461	52,784
	10 20 30 40 50 60 MVLENILEKI VKSKINWIKH RKKIQPLSSF QHNITLSDRN FIQALKNIHP ALILEFKKHS 70 80 90 100 110 120 PSLGILNDFN PEFVAKIYKK YASAISVLTD EKYFHGKFEF IPIIRNIAVQ QPILCKDFFI 130 140 150 160 170 180 DPYQIYLARY YQADSILLML SILKDNQYRA LEKLAYSLNM AVLTEINNKM ELDRAINLNA 190 200 210 220 230 240 KIIGINNRNL KNFSISTSNT YKLASKISKN TIVISESGIN SYNQLRKFKN LVQGFLIGSA 250 260 270 280 290 300 LMSKKDLEHA VHKIITGNNK ICGLTRVEDA RMSKDFGAIY GGFIFCKSSK RYVNLKKAMN 310 320 330 340 350 360 ITKNVHMKYI GVFCNENIST ISYIIDKIPL YAIQLHGNEN QFYIDCLKKK IPKCVRVWKA 370 380 390 400 410 420 ISLNGEKKHA NNLFDNVNKH VFDNIHGGSG TPFNWYLLKN YNLKNVILAG GLNIKNCISA 430 440 450 460 SDLGCFGLDF NSGIEISPGL KDKKKTFLIF RSLREHKTII H

References

[1]	"Genetics of the tryptophan biosynthetic pathway of the prokaryotic endosymbiont (Buchnera) of the aphid Schlechtendalia chinensis." Lai C.-Y., Baumann P., Moran N.A. Insect Mol. Biol. 4:47-59(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U09185 Genomic DNA. Translation: AAA92795.1.
3D structure databases
ProteinModelPortal	Q44603.
SMR	Q44603. Positions 3-261.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00035; UER00042. UPA00035; UER00043.
Family and domain databases
Gene3D	3.20.20.70. 2 hits.
HAMAP	MF_00134_B. IGPS_B. Fused. MF_00135. PRAI. Fused.
InterPro	IPR013785. Aldolase_TIM. IPR013798. Indole-3-glycerol_P_synth. IPR001468. Indole-3-GlycerolPSynthase_CS. IPR001240. PRAI_dom. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Pfam	PF00218. IGPS. 1 hit. PF00697. PRAI. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 2 hits.
PROSITE	PS00614. IGPS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPC_BUCSC

Accession

Primary (citable) accession number: Q44603

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program