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Protein

Anthranilate synthase component 1

Gene

trpE

Organism
Buchnera aphidicola subsp. Schlechtendalia chinensis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia (By similarity).By similarity

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Enzyme regulationi

Feedback inhibited by tryptophan.By similarity

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Anthranilate synthase component 1 (trpE), Anthranilate synthase component 1 (XW81_02705)
  2. Anthranilate phosphoribosyltransferase (trpD), Anthranilate phosphoribosyltransferase (trpD)
  3. Tryptophan biosynthesis protein TrpCF (trpC)
  4. Tryptophan biosynthesis protein TrpCF (trpC), Indole-3-glycerol phosphate synthase (XW81_01310)
  5. Tryptophan synthase beta chain (trpB), Tryptophan synthase alpha chain (trpA), Tryptophan synthase beta chain (trpB), Tryptophan synthase alpha chain (trpA)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40TryptophanBy similarity1
Metal bindingi360MagnesiumBy similarity1
Binding sitei448ChorismateBy similarity1
Binding sitei468ChorismateBy similarity1
Binding sitei484Chorismate; via amide nitrogenBy similarity1
Metal bindingi497MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.

Names & Taxonomyi

Protein namesi
Recommended name:
Anthranilate synthase component 1 (EC:4.1.3.27)
Short name:
AS
Short name:
ASI
Gene namesi
Name:trpE
OrganismiBuchnera aphidicola subsp. Schlechtendalia chinensis
Taxonomic identifieri118110 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001540871 – 530Anthranilate synthase component 1Add BLAST530

Interactioni

Subunit structurei

Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ44598.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni290 – 292Tryptophan bindingBy similarity3
Regioni327 – 328Chorismate bindingBy similarity2
Regioni482 – 484Chorismate bindingBy similarity3

Sequence similaritiesi

Family and domain databases

Gene3Di3.60.120.10. 1 hit.
InterProiIPR005801. ADC_synthase.
IPR019999. Anth_synth_I-like.
IPR006805. Anth_synth_I_N.
IPR005257. Anth_synth_I_TrpE.
IPR015890. Chorismate_C.
[Graphical view]
PfamiPF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
[Graphical view]
PIRSFiPIRSF001373. TrpE. 1 hit.
PRINTSiPR00095. ANTSNTHASEI.
SUPFAMiSSF56322. SSF56322. 1 hit.
TIGRFAMsiTIGR00565. trpE_proteo. 1 hit.

Sequencei

Sequence statusi: Complete.

Q44598-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCEHLKSMDV LKVETFYQPN PTAIFHHICR NRSNTLLLES AEINNKKNLE
60 70 80 90 100
SMMIVDSALR ISALNMVVTL EALTKNGESL LPVFKSLLPK EVRILSNDNF
110 120 130 140 150
LKIKFPSIVK DIDEDKKLHV LSIFDSIRFL INSVKNNKGS KSMFFGGLFA
160 170 180 190 200
YDLVSSFENL PKLKTSQSCP DFCFYLSEIL LVLNHKRRTC YIQASLFSSS
210 220 230 240 250
NLERNKLQHR LLEVKNKLSQ NFYPLESTSL KKMILKCNRT DREYEKIIKE
260 270 280 290 300
MKKSIMIGEI FQVVPSRKFY LPCTNSLAAY DVLKKNNPSP YMFFMQDSNF
310 320 330 340 350
TLFGASPESS LKYDTLSRKI EIYPIAGTRP RARKIDGSID LDLDSRIELE
360 370 380 390 400
MRTNHKELSE HLMLVDLARN DLAKICDPGT RYVADLTQVD KYSHVMHLVS
410 420 430 440 450
RVVGKLRLDL DVFHAYQACM NMGTLSGAPK IRAMELISNA EKEKRGSYGG
460 470 480 490 500
SIGYFTASGT LDMCIIIRSA YVENNIATIQ VGAGIVLDSI PQLEVDESKN
510 520 530
KAKAVLQAIS ESHSCHIELN IENERHTFAG
Length:530
Mass (Da):60,189
Last modified:November 1, 1996 - v1
Checksum:i2AE8702A6DBE7AB5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09184 Genomic DNA. Translation: AAC31215.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09184 Genomic DNA. Translation: AAC31215.1.

3D structure databases

ProteinModelPortaliQ44598.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.

Family and domain databases

Gene3Di3.60.120.10. 1 hit.
InterProiIPR005801. ADC_synthase.
IPR019999. Anth_synth_I-like.
IPR006805. Anth_synth_I_N.
IPR005257. Anth_synth_I_TrpE.
IPR015890. Chorismate_C.
[Graphical view]
PfamiPF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
[Graphical view]
PIRSFiPIRSF001373. TrpE. 1 hit.
PRINTSiPR00095. ANTSNTHASEI.
SUPFAMiSSF56322. SSF56322. 1 hit.
TIGRFAMsiTIGR00565. trpE_proteo. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRPE_BUCSC
AccessioniPrimary (citable) accession number: Q44598
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.