Reviewed, UniProtKB/Swiss-Prot Q44524 (DHNO_AGRVI)

Names and origin

Protein names

Recommended name:
    D-nopaline dehydrogenase
    EC=1.5.1.19
Alternative name(s):
    Nopaline synthase

Gene names

Name:

nos

Encoded on

Plasmid pTiAB4

Organism

Agrobacterium vitis (Rhizobium vitis)

Taxonomic identifier

373 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	N(2)-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O = L-arginine + 2-oxoglutarate + NADPH.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the lysopine/nopaline/octopine/opine/vitopine dehydrogenases family.

Ontologies

Keywords
Disease	Crown gall tumor
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	glycerol-3-phosphate catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	D-nopaline dehydrogenase activity Inferred from electronic annotation. Source: EC NAD or NADH binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 412	412	D-nopaline dehydrogenase		PRO_0000179984

Sequences

	Sequence		Length	Mass (Da)
	Q44524-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: CEBD6858EA2AE395	FASTA	412	45,279
	10 20 30 40 50 60 MRLASSSTSL PVPAAGHHPL PLTVGVLGSG HAGTALAAWF ASRHVSTALW APADHPGSIS 70 80 90 100 110 120 AIKANEGVVT TEGVINGSFT VSASDDLLAV IRSSHLLIIV TRADVHDSFV SELANFNGEL 130 140 150 160 170 180 AEKDILVVCG HGFSMKYERQ LQCKRILETD NSPTTSKLSD KKKCKVNIKE MKASFGLSCF 190 200 210 220 230 240 PIHRNDVGVI DLPEDIKIIF AQLFTARIIP IPPLQVLFFS NYITHAVAAV MNIGSVRDPV 250 260 270 280 290 300 NSLTKRAEQW LLELDERTPR AETGFFFYGE GSNTYVCKVQ EQIDQERRKV AKACGLHLNS 310 320 330 340 350 360 LLQECNDEYG TDYATLREYC LAPSPHNVHY ACPDNMEHRY FSEELCSLED IAAIATITKI 370 380 390 400 410 EMPLTRAFIN IIHAGKGNFP PTDKTSSVIG NFRSGDLIRF GATIFVKDEK MK

References

[1]	"Agrobacterium vitis nopaline Ti plasmid pTiAB4: relationship to other Ti plasmids and T-DNA structure." Otten L., de Ruffray P. Mol. Gen. Genet. 245:493-505(1994) [PubMed: 7808399] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AB4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X77327 Genomic DNA. Translation: CAA54543.1.
PIR	S41895.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.5.1.19. 97084.
Family and domain databases
InterPro	IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. IPR011128. NAD-dep_Gly3P_DH_N. IPR003421. Opine_DH. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
Pfam	PF01210. NAD_Gly3P_dh_N. 1 hit. PF02317. Octopine_DH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHNO_AGRVI

Accession

Primary (citable) accession number: Q44524

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)