Reviewed, UniProtKB/Swiss-Prot Q44473 (KPYK2_AGRVI)

Names and origin

Protein names

Recommended name:
    Pyruvate kinase
      Short name=PK
    EC=2.7.1.40

Gene names

Name:

ttuE

Encoded on

Plasmid pTrAB4

Organism

Agrobacterium vitis (Rhizobium vitis)

Taxonomic identifier

373 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium. Potassium.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer By similarity.
Induction	By tartrate.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Pyruvate
Molecular function	Kinase Transferase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 482	482	Pyruvate kinase		PRO_0000112052
Sites
	Active site	220	1	By similarity
	Metal binding	222	1	Magnesium By similarity
	Metal binding	243	1	Magnesium By similarity
	Metal binding	244	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q44473-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 2D2B614589CB46E7	FASTA	482	51,678
	10 20 30 40 50 60 MFIRNNRRSK IVATVGPASS SPDMLRSLFL AGVDTFRLNF SHGARADHAE VYRNIRALEQ 70 80 90 100 110 120 EHDAAIAVLQ DLQGPKIRIG VLAHGRLDLA RGSTIGFILG REGGEGMNDI PLPHREIFEV 130 140 150 160 170 180 AVPGMDLLID DGRIKVRIME VMDGRLVCEV LNGGALSNRK GVNVPGAVLD ISPLTAKDRE 190 200 210 220 230 240 DLEFGLELGV DWVALSFVQR ARDMIEARSL VGDRAGLIAK IEKPSALDDI EDIVRLSDSV 250 260 270 280 290 300 MVARGDLGVE IPPEDVPGKQ KEIIRACRLA AKPVIVATQM LDSMVSSPTP TRAEASDVAG 310 320 330 340 350 360 AIYDGADAVM LSAETATGAY PVEAVEIMNR IIEKTEKHKH YRPILEATEP DVAQSPPHAV 370 380 390 400 410 420 ATAAANVAVA LGSPVVVAYT SSGTTAARIS RARPALPILA LTPSEQVARR LNMFWGVVGV 430 440 450 460 470 480 RSQDVHTYEA SLIHAQQAVQ EAKLASPSDH IVIVAGFPFA QQGSTNNLRV VQIAATDNLE IA

References

[1]	"Sequence and mutational analysis of a tartrate utilization operon from Agrobacterium vitis." Crouzet P., Otten L. J. Bacteriol. 177:6518-6526(1995) [PubMed: 7592429] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AB4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U25634 Genomic DNA. Translation: AAA68700.1.
3D structure databases
HSSP	HSSP built from PDB template 1E0T based on UniProtKB P14178.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.1.40. 97084.
Family and domain databases
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit. G3DSA:3.40.1380.20. Pyrv_Knase_a/b. 1 hit.
PANTHER	PTHR11817. Pyruvate_kinase. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
ProDom	PD001009. Pyruvate_kinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK2_AGRVI

Accession

Primary (citable) accession number: Q44473

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)