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Protein

Malto-oligosyltrehalose trehalohydrolase

Gene

treZ

Organism
Arthrobacter sp. (strain Q36)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan.By similarity

Pathwayi: trehalose biosynthesis

This protein is involved in the pathway trehalose biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway trehalose biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei267NucleophileBy similarity1
Active sitei304Proton donorBy similarity1
Sitei400Transition state stabilizerBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-6022.
UniPathwayiUPA00299.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Malto-oligosyltrehalose trehalohydrolase (EC:3.2.1.141By similarity)
Short name:
MTHase
Alternative name(s):
4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase
Maltooligosyl trehalose trehalohydrolase
Gene namesi
Name:treZ
OrganismiArthrobacter sp. (strain Q36)
Taxonomic identifieri104027 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrococcaceaeArthrobacter

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000543211 – 598Malto-oligosyltrehalose trehalohydrolaseAdd BLAST598

Structurei

3D structure databases

ProteinModelPortaliQ44316.
SMRiQ44316.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni265 – 270Substrate bindingBy similarity6
Regioni329 – 333Substrate bindingBy similarity5
Regioni399 – 404Substrate bindingBy similarity6

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiView protein in InterPro
IPR022567. DUF3459.
IPR006047. Glyco_hydro_13_cat_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR012768. Trehalose_TreZ.
PfamiView protein in Pfam
PF00128. Alpha-amylase. 1 hit.
PF11941. DUF3459. 1 hit.
PIRSFiPIRSF006337. Trehalose_TreZ. 1 hit.
SMARTiView protein in SMART
SM00642. Aamy. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR02402. trehalose_TreZ. 1 hit.

Sequencei

Sequence statusi: Complete.

Q44316-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHTYPREAA KPVLGPARYD VWAPNAESVT LLAGGERYAM QRRAETGPED
60 70 80 90 100
AGWWTAAGAP TDGNVDYGYL LDGDETPLPD PRTRRQPDGV HALSRTFDPS
110 120 130 140 150
AYSWQDDAWQ GRELQGAVIY ELHLGTFTPE GTLEAAAGKL DYLAGLGVDF
160 170 180 190 200
IELLPVNAFN GTHNWGYDGV QWFAVHEAYG GPEAYQRFVD AAHAAGLGVI
210 220 230 240 250
QDVVYNHLGP SGNYLPRFGP YLKQGEGNTW GDSVNLDGPG SDHVRRYILD
260 270 280 290 300
NLAMWLRDYR VDGLRLDAVH ALKDERAVHI LEDFGALADQ ISAEVGRPLT
310 320 330 340 350
LIAESDLNNP RLLYPRDVNG YGLEGQWSDD FHHAVHVNVT GETTGYYSDF
360 370 380 390 400
DSLAALAKVL RDGFFHDGSY SSFRERHHGR PINFSAVHPA ALVVCSQNHD
410 420 430 440 450
QIGNRATGDR LSQTLPYGSL ALAAVLTLTG PFTPMLLMGE EYGASTPWQF
460 470 480 490 500
FTSHPEPELG KATAEGRIKE FERMGWDPAV VPDPQDPETF RRSKLDWAEA
510 520 530 540 550
AEGDHARLLE LYRSLTALRR STPDLTKLGF EDTQVAFDED ARWLRFRRGG
560 570 580 590
VQVLLNFSEQ PVSLDGAGTA LLLATDDAVR LEGERAELGP LSAAVVSD
Length:598
Mass (Da):65,832
Last modified:November 1, 1996 - v1
Checksum:i8B5C610AD3766947
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63343 Genomic DNA. Translation: BAA09668.1.
PIRiS65770.

Similar proteinsi

Entry informationi

Entry nameiTREZ_ARTSQ
AccessioniPrimary (citable) accession number: Q44316
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: November 1, 1996
Last modified: August 30, 2017
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families