Q44316 (TREZ_ARTSQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malto-oligosyltrehalose trehalohydrolase
Short name=MTHase
EC=3.2.1.141

Alternative name(s):
4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase
Maltooligosyl trehalose trehalohydrolase

Gene names

Name:

treZ

Organism

Arthrobacter sp. (strain Q36)

Taxonomic identifier

104027 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	598 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan.
Pathway	Glycan biosynthesis; trehalose biosynthesis.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological_process	trehalose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 598	598	Malto-oligosyltrehalose trehalohydrolase		PRO_0000054321
Regions
	Region	329 – 333	5	Substrate binding By similarity
Sites
	Active site	267	1	Nucleophile By similarity
	Active site	304	1	Proton donor By similarity
	Site	400	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q44316 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 8B5C610AD3766947	FASTA	598	65,832
	10 20 30 40 50 60 MTHTYPREAA KPVLGPARYD VWAPNAESVT LLAGGERYAM QRRAETGPED AGWWTAAGAP 70 80 90 100 110 120 TDGNVDYGYL LDGDETPLPD PRTRRQPDGV HALSRTFDPS AYSWQDDAWQ GRELQGAVIY 130 140 150 160 170 180 ELHLGTFTPE GTLEAAAGKL DYLAGLGVDF IELLPVNAFN GTHNWGYDGV QWFAVHEAYG 190 200 210 220 230 240 GPEAYQRFVD AAHAAGLGVI QDVVYNHLGP SGNYLPRFGP YLKQGEGNTW GDSVNLDGPG 250 260 270 280 290 300 SDHVRRYILD NLAMWLRDYR VDGLRLDAVH ALKDERAVHI LEDFGALADQ ISAEVGRPLT 310 320 330 340 350 360 LIAESDLNNP RLLYPRDVNG YGLEGQWSDD FHHAVHVNVT GETTGYYSDF DSLAALAKVL 370 380 390 400 410 420 RDGFFHDGSY SSFRERHHGR PINFSAVHPA ALVVCSQNHD QIGNRATGDR LSQTLPYGSL 430 440 450 460 470 480 ALAAVLTLTG PFTPMLLMGE EYGASTPWQF FTSHPEPELG KATAEGRIKE FERMGWDPAV 490 500 510 520 530 540 VPDPQDPETF RRSKLDWAEA AEGDHARLLE LYRSLTALRR STPDLTKLGF EDTQVAFDED 550 560 570 580 590 ARWLRFRRGG VQVLLNFSEQ PVSLDGAGTA LLLATDDAVR LEGERAELGP LSAAVVSD

References

[1]	"Cloning and sequencing of trehalose biosynthesis genes from Arthrobacter sp. Q36." Maruta K., Hattori K., Nakada T., Kubota M., Sugimoto T., Kurimoto M. Biochim. Biophys. Acta 1289:10-13(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	D63343 Genomic DNA. Translation: BAA09668.1.
PIR	S65770.
3D structure databases
ProteinModelPortal	Q44316.
ModBase	Search...
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-6022.
UniPathway	UPA00299.
Family and domain databases
Gene3D	2.60.40.10. 1 hit. 3.20.20.80. 2 hits.
InterPro	IPR022567. DUF3459. IPR015902. Glyco_hydro_13. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR012768. Trehalose_TreZ. [Graphical view]
PANTHER	PTHR10357. PTHR10357. 1 hit. PTHR10357:SF21. PTHR10357:SF21. 1 hit.
Pfam	PF00128. Alpha-amylase. 2 hits. PF02922. CBM_48. 1 hit. PF11941. DUF3459. 1 hit. [Graphical view]
PIRSF	PIRSF006337. Trehalose_TreZ. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR02402. trehalose_TreZ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TREZ_ARTSQ

Accession

Primary (citable) accession number: Q44316

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program