Reviewed, UniProtKB/Swiss-Prot Q44315 (TREY_ARTSQ)

Names and origin

Protein names

Recommended name:
    Maltooligosyl trehalose synthase
    EC=5.4.99.15
Alternative name(s):
    (1,4)-alpha-D-glucan 1-alpha-D-glucosylmutase

Gene names

Name:

treY

Organism

Arthrobacter sp. (strain Q36)

Taxonomic identifier

104027 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	775 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the conversion of maltooligosaccharide into the non-reducing saccharide, maltooligosyl trehalose (alpha-maltooligosyl alpha-D-glucoside) by intramolecular transglycosylation.
Catalytic activity	4-((1->4)-alpha-D-glucosyl)(n-1)-D-glucose = 1-alpha-D-((1->4)-alpha-D-glucosyl)(n-1)-alpha-D-glucopyranoside.
Subunit structure	Monomer.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Molecular function	Isomerase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	(1,4)-alpha-D-glucan 1-alpha-D-glucosylmutase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 775	775	Maltooligosyl trehalose synthase		PRO_0000054341

Sequences

	Sequence		Length	Mass (Da)
	Q44315-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 1BBD23024B2280FC	FASTA	775	85,356
	10 20 30 40 50 60 MRTPVSTYRL QIRKGFTLFD AAKTVPYLHS LGVDWVYLSP VLTAEQGSDH GYDVTDPSAV 70 80 90 100 110 120 DPERGGPEGL AAVSKAARAA GMGVLIDIVP NHVGVATPAQ NPWWWSLLKE GRQSRYAEAF 130 140 150 160 170 180 DVDWDLAGGR IRLPVLGSDD DLDQLEIRDG ELRYYDHRFP LAEGTYAEGD APRDVHARQH 190 200 210 220 230 240 YELIGWRRAD NELNYRRFFA VNTLAGVRVE IPAVFDEAHQ EVVRWFREDL ADGLRIDHPD 250 260 270 280 290 300 GLADPEGYLK RLREVTGGAY LLIEKILEPG EQLPASFECE GTTGYDALAD VDRVLVDPRG 310 320 330 340 350 360 QEPLDRLDAS LRGGEPADYQ DMIRGTKRRI TDGILHSEIL RLARLVPGDA NVSIDAGADA 370 380 390 400 410 420 LAEIIAAFPV YRTYLPEGAE VLKEACELAA RRRPELDQAI QALQPLLLDT DLELARRFQQ 430 440 450 460 470 480 TSGMVMAKGV EDTAFFRYNR LGTLTEVGAD PTEFAVEPDE FHARLARRQA ELPLSMTTLS 490 500 510 520 530 540 THDTKRSEDT RARISVISEV AGDWEKALNR LRDLAPLPDG PLSALLWQAI AGAWPASRER 550 560 570 580 590 600 LQYYALKAAR EAGNSTNWTD PAPAFEEKLK AAVDAVFDNP AVQAEVEALV ELLEPYGASN 610 620 630 640 650 660 SLAAKLVQLT MPGVPDVYQG TEFWDRSLTD PDNRRPFSFD DRRAALEQLD AGDLPASFTD 670 680 690 700 710 720 ERTKLLVTSR ALRLRRDRPE LFTGYRPVLA SGPAAGHLLA FDRGTAAAPG ALTLATRLPY 730 740 750 760 770 GLEQSGGWRD TAVELNTAMK DELTGAGFGP GAVKIADIFR SFPVALLVPQ TGGES

References

[1]	"Cloning and sequencing of trehalose biosynthesis genes from Arthrobacter sp. Q36." Maruta K., Hattori K., Nakada T., Kubota M., Sugimoto T., Kurimoto M. Biochim. Biophys. Acta 1289:10-13(1996) [PubMed: 8605217] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Purification and properties of a novel enzyme, maltooligosyl trehalose synthase, from Arthrobacter sp. Q36." Nakada T., Maruta K., Tsusaki K., Kubota M., Chaen H., Sugimoto T., Kurimoto M., Tsujisaka Y. Biosci. Biotechnol. Biochem. 59:2210-2214(1995) [PubMed: 8611744] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D63343 Genomic DNA. Translation: BAA09667.1.
PIR	S65769.
3D structure databases
HSSP	HSSP built from PDB template 1IV8 based on UniProtKB Q53688.
ModBase	Search...
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-6021.
Family and domain databases
InterPro	IPR006047. Glyco_hydro_13_cat. IPR013781. Glyco_hydro_sg_catalytic. IPR012767. Trehalose_TreY. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. [Graphical view]
TIGRFAMs	TIGR02401. trehalose_TreY. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TREY_ARTSQ

Accession

Primary (citable) accession number: Q44315

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)