Q44278 (GYRB_ACIS7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit B
EC=5.99.1.3

Gene names

Name:

gyrB

Organism

Acinetobacter sp. (strain ATCC 33308 / BD413 ErpE27)

Taxonomic identifier

68992 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	216 AA.
Sequence status	Fragments.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01898
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01898
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Subcellular location	Cytoplasm Potential HAMAP MF_01898.
Sequence similarities	Belongs to the type II topoisomerase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›216	›216	DNA gyrase subunit B HAMAP MF_01898		PRO_0000145286
Experimental info
	Non-adjacent residues	116 – 117	2
	Non-terminal residue	1	1
	Non-terminal residue	216	1

Sequences

	Sequence		Length	Mass (Da)
	Q44278 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 48186007AD1D301B	FASTA	216	23,742
	10 20 30 40 50 60 SHKVSGGLHG VGVSVVNALS SKLQLTIHRA GQIHEQEYQH GDPQYPLKVV GETSTTGTTV 70 80 90 100 110 120 RFWPSGDTFS QTIFNVDILA RRLRELSFLN AGVKIVLRDE RVNFEHIYAY EGGLSEKSAL 130 140 150 160 170 180 DIAGLPGKLA DCQEKDPALS ELYLVEGDSP GGSAKQGRNR KMQAILPLKG KILNVERARF 190 200 210 DKMISSQEVG TLITALGCGI GREEYNPDKL RYHKII

References

[1]	"Phylogenetic analysis of Acinetobacter strains based on the nucleotide sequences of gyrB genes and on the amino acid sequences of their products." Yamamoto S., Harayama S. Int. J. Syst. Bacteriol. 46:506-511(1996) [PubMed: 8934907] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D73441 Genomic DNA. Translation: BAA11166.1. D73426 Genomic DNA. Translation: BAA11151.1.
3D structure databases
ProteinModelPortal	Q44278.
SMR	Q44278. Positions 1-114.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_01898. GyrB. [Tree]
InterPro	IPR003594. ATPase-like_ATP-bd. IPR001241. Topo_IIA. IPR013759. Topo_IIA_B/N_ab. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit. G3DSA:3.40.50.670. Topo_IIA_B/N_ab. 1 hit.
SMART	SM00433. TOP2c. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF56719. Topo_IIA_cen. 1 hit.
PROSITE	PS00177. TOPOISOMERASE_II. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GYRB_ACIS7

Accession

Primary (citable) accession number: Q44278
Secondary accession number(s): Q44269

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program