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Q44277 (GYRB_ACIS6) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA gyrase subunit B

EC=5.99.1.3
Gene names
Name:gyrB
OrganismAcinetobacter sp. (strain ATCC 31012)
Taxonomic identifier68991 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacter

Protein attributes

Sequence length216 AA.
Sequence statusFragments.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01898

Catalytic activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01898

Subunit structure

Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.

Subcellular location

Cytoplasm Potential HAMAP MF_01898.

Sequence similarities

Belongs to the type II topoisomerase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionIsomerase
Topoisomerase
Gene Ontology (GO)
   Biological processDNA topological change

Inferred from electronic annotation. Source: InterPro

   Cellular componentchromosome

Inferred from electronic annotation. Source: InterPro

cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA topoisomerase (ATP-hydrolyzing) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – ›216›216DNA gyrase subunit B HAMAP MF_01898
PRO_0000145285

Experimental info

Non-adjacent residues116 – 1172
Non-terminal residue11
Non-terminal residue2161

Sequences

Sequence LengthMass (Da)Tools
Q44277 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: C73D4B3B7A7A6484

FASTA21623,872
        10         20         30         40         50         60 
SYKVSGGLHG VGVSVVNALS KKLHLTIHRA GQIHEQEYAH GDPQYPLKVV GETDTSGTTV 

        70         80         90        100        110        120 
RFWPSELTFS QTIFSVDILA RRLRELSFLN AGVRIVLRDE RVNLEHIYDY EVGLSEKSAL 

       130        140        150        160        170        180 
DIAGLPGKLA DCQEKDPALS ELYLVEGDSA GGSAKQGRNR KMQAILPLKG KILNVERARF 

       190        200        210 
DKMISSQEVG TLITALGCGI GREEYNPDKL RYHKII 

« Hide

References

[1]"Phylogenetic analysis of Acinetobacter strains based on the nucleotide sequences of gyrB genes and on the amino acid sequences of their products."
Yamamoto S., Harayama S.
Int. J. Syst. Bacteriol. 46:506-511(1996) [PubMed: 8934907] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D73440 Genomic DNA. Translation: BAA11165.1.
D73425 Genomic DNA. Translation: BAA11150.1.

3D structure databases

ProteinModelPortalQ44277.
SMRQ44277. Positions 1-114.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

HAMAPMF_01898. GyrB.
[Tree]
InterProIPR003594. ATPase-like_ATP-bd.
IPR001241. Topo_IIA.
IPR013759. Topo_IIA_B/N_ab.
IPR013760. Topo_IIA_cen.
IPR018522. TopoIIA_CS.
[Graphical view]
Gene3DG3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
G3DSA:3.40.50.670. Topo_IIA_B/N_ab. 1 hit.
SMARTSM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMSSF55874. ATP_bd_ATPase. 1 hit.
SSF56719. Topo_IIA_cen. 1 hit.
PROSITEPS00177. TOPOISOMERASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGYRB_ACIS6
AccessionPrimary (citable) accession number: Q44277
Secondary accession number(s): Q60168
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families