Reviewed, UniProtKB/Swiss-Prot Q44277 (GYRB_ACIS6)

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit B
    EC=5.99.1.3

Gene names

Name:

gyrB

Organism

Acinetobacter sp. (strain ATCC 31012)

Taxonomic identifier

68991 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	216 AA.
Sequence status	Fragments.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Sequence similarities	Belongs to the type II topoisomerase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›216	›216	DNA gyrase subunit B		PRO_0000145285
Experimental info
	Non-adjacent residues	116 – 117	2
	Non-terminal residue	1	1
	Non-terminal residue	216	1

Sequences

	Sequence		Length	Mass (Da)
	Q44277-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: C73D4B3B7A7A6484	FASTA	216	23,872
	10 20 30 40 50 60 SYKVSGGLHG VGVSVVNALS KKLHLTIHRA GQIHEQEYAH GDPQYPLKVV GETDTSGTTV 70 80 90 100 110 120 RFWPSELTFS QTIFSVDILA RRLRELSFLN AGVRIVLRDE RVNLEHIYDY EVGLSEKSAL 130 140 150 160 170 180 DIAGLPGKLA DCQEKDPALS ELYLVEGDSA GGSAKQGRNR KMQAILPLKG KILNVERARF 190 200 210 DKMISSQEVG TLITALGCGI GREEYNPDKL RYHKII

References

[1]	"Phylogenetic analysis of Acinetobacter strains based on the nucleotide sequences of gyrB genes and on the amino acid sequences of their products." Yamamoto S., Harayama S. Int. J. Syst. Bacteriol. 46:506-511(1996) [PubMed: 8934907] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	D73440 Genomic DNA. Translation: BAA11165.1. D73425 Genomic DNA. Translation: BAA11150.1.
3D structure databases
SMR	Q44277. Positions 1-114, 122-216.
ModBase	Search...
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR001241. Topo_IIA_B/N. IPR013759. Topo_IIA_B/N_ab. IPR013760. Topo_IIA_cen. IPR018522. TopoIIA_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.670. Topo_IIA_B/N_ab. 1 hit.
SMART	SM00433. TOP2c. 1 hit. [Graphical view]
PROSITE	PS00177. TOPOISOMERASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GYRB_ACIS6

Accession

Primary (citable) accession number: Q44277
Secondary accession number(s): Q60168

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 19, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)